Features
Primers
Cut sites
CDS
misc_feature
primer_bind
promoter
protein_bind
rep_origin
Circle
Line
Sequence
Zoom
Reset
500
1000
1500
2000
2500
3000
lacZα
GFP
ori
AmpR
M13 fwd
MCS
GCATGC junction
AAGCTT junction
M13 rev
lac operator
lac promoter
AmpR promoter
GFP forward (447 .. 472)
GFP reverse (1138 .. 1163)
Colony PCR forward (390 .. 412)
Colony PCR reverse (1188 .. 1217)
Sequencing forward (325 .. 341)
Sequencing reverse (1288 .. 1307)
SphI
(445)
HindIII
(1164)
pUC19-GFP
3403 bp
500
1000
1500
2000
2500
3000
lacZα
MCS
GFP
ori
AmpR
AmpR promoter
M13 fwd
GCATGC junction
AAGCTT junction
M13 rev
lac operator
lac promoter
GFP forward (447 .. 472)
GFP reverse (1138 .. 1163)
Colony PCR forward (390 .. 412)
Colony PCR reverse (1188 .. 1217)
Sequencing forward (325 .. 341)
Sequencing reverse (1288 .. 1307)
SphI
(445)
HindIII
(1164)
pUC19-GFP
3403 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
3400
lacZα
MCS
GFP
ori
AmpR
AmpR promoter
M13 fwd
GCATGC junction
AAGCTT junction
M13 rev
lac operator
lac promoter
GFP forward (447 .. 472)
GFP reverse (1138 .. 1163)
Colony PCR forward (390 .. 412)
Colony PCR reverse (1188 .. 1217)
Sequencing forward (325 .. 341)
Sequencing reverse (1288 .. 1307)
SphI
(445)
HindIII
(1164)
pUC19-GFP
3403 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
3400
lacZα
M13 fwd
MCS
GFP
ori
AmpR
AmpR promoter
GCATGC junction
AAGCTT junction
M13 rev
lac operator
lac promoter
GFP forward (447 .. 472)
GFP reverse (1138 .. 1163)
Colony PCR forward (390 .. 412)
Colony PCR reverse (1188 .. 1217)
Sequencing forward (325 .. 341)
Sequencing reverse (1288 .. 1307)
SphI
(445)
HindIII
(1164)
pUC19-GFP
3403 bp
50
100
150
200
250
300
350
400
450
500
550
600
650
700
750
800
850
900
950
1000
1050
1100
1150
1200
1250
1300
1350
1400
1450
1500
1550
1600
1650
1700
1750
1800
1850
1900
1950
2000
2050
2100
2150
2200
2250
2300
2350
2400
2450
2500
2550
2600
2650
2700
2750
2800
2850
2900
2950
3000
3050
3100
3150
3200
3250
3300
3350
3400
lacZα
M13 fwd
MCS
GCATGC junction
GFP
M13 rev
ori
AmpR
AmpR promoter
AAGCTT junction
lac operator
lac promoter
GFP forward (447 .. 472)
GFP reverse (1138 .. 1163)
Colony PCR forward (390 .. 412)
Colony PCR reverse (1188 .. 1217)
Sequencing forward (325 .. 341)
Sequencing reverse (1288 .. 1307)
SphI
(445)
HindIII
(1164)
pUC19-GFP
3403 bp
Both strands
Copy
10 20 30 40 50 60
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
AGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGT
60
70 80 90 100 110 120
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
GTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
120
130 140 150 160 170 180
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
AACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACG
180
Ile Arg Cys Leu Leu Asn Tyr Gln Ser His
Ter
lacZα
190 200 210 220 230 240
ACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCC
TGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGG
240
Val Met His Pro Thr Phe Tyr Arg Val Ser Ala Tyr Pro Ser Phe Val Ala Asp Pro Ala
lacZα
250 260 270 280 290 300
ATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATA
300
Met Arg Trp Glu Pro Gln Ala Val Thr Pro Phe Pro Ser Arg His Pro Gly Arg Arg
Ter
lacZα
310 320 330 340 350 360
TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGT
ATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCCCA
360
Ala Leu Gln Arg Phe Pro Ser Thr Ser Cys Pro Ser Thr Pro Tyr Arg Trp Pro
Ter Ter
lacZα
Sequencing forward (325 .. 341)
370 380 390 400 410 420
TTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGAT
AAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTTAAGCTCGAGCCATGGGCCCCTA
420
Lys Gly Leu Ser Thr Thr Phe Arg Arg Gly Thr Phe Glu Leu Glu Thr Gly Pro Ser
Ter
lacZα
M13 fwd
MCS
Colony PCR forward (390 .. 412)
430 440 450 460 470 480
CCTCTAGAGTCGACCTGCAGGCATGCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTG
GGAGATCTCAGCTGGACGTCCGTACGTACTCATTTCCTCTTCTTGAAAAGTGACCTCAAC
480
Gly Arg Ser Asp Val Gln Leu Cys Ser
lacZα
MCS
GCATGC junction
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val
GFP
GFP forward (447 .. 472)
SphI
490 500 510 520 530 540
TCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAG
AGGGTTAAGAACAACTTAATCTACCACTACAATTACCCGTGTTTAAAAGACAGTCACCTC
540
Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly
GFP
550 560 570 580 590 600
AGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAA
TCCCACTTCCACTACGTTGTATGCCTTTTGAATGGGAATTTAAATAAACGTGATGACCTT
600
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly
GFP
610 620 630 640 650 660
AACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTTT
TTGATGGACAAGGTACCGGTTGTGAACAGTGATGAAAGAGAATACCACAAGTTACGAAAA
660
Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ser Tyr Gly Val Gln Cys Phe
GFP
670 680 690 700 710 720
CAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTT
GTTCTATGGGTCTAGTATACTTTGCCGTACTGAAAAAGTTCTCACGGTACGGGCTTCCAA
720
Ser Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly
GFP
730 740 750 760 770 780
ATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAG
TACATGTCCTTTCTTGATATAAAAAGTTTCTACTGCCCTTGATGTTCTGTGCACGACTTC
780
Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu
GFP
790 800 810 820 830 840
TCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAG
AGTTCAAACTTCCACTATGGGAACAATTATCTTAGCTCAATTTTCCATAACTAAAATTTC
840
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys
GFP
850 860 870 880 890 900
AAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACA
TTCTACCTTTGTAAGAACCTGTGTTTAACCTTATGTTGATATTGAGTGTGTTACATATGT
900
Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr
GFP
910 920 930 940 950 960
TCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTG
AGTACCGTCTGTTTGTTTTCTTACCTTAGTTTCAATTGAAGTTTTAATCTGTGTTGTAAC
960
Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile
GFP
970 980 990 1000 1010 1020
AAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCC
TTCTACCTTCGCAAGTTGATCGTCTGGTAATAGTTGTTTTATGAGGTTAACCGCTACCGG
1020
Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly
GFP
1030 1040 1050 1060 1070 1080
CTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCA
GACAGGAAAATGGTCTGTTGGTAATGGACAGGTGTGTTAGACGGGAAAGCTTTCTAGGGT
1080
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro
GFP
1090 1100 1110 1120 1130 1140
ACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATG
TGCTTTTCTCTCTGGTGTACCAGGAAGAACTCAAACATTGTCGACGACCCTAATGTGTAC
1140
Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His
GFP
GFP reverse (1138 .. 1163)
1150 1160 1170 1180 1190 1200
GCATGGATGAACTATACAAATAGAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTG
CGTACCTACTTGATATGTTTATCTTCGAACCGCATTAGTACCAGTATCGACAAAGGACAC
1200
Ser Pro Thr Ile Met Thr Met
lacZα
MCS
Gly Met Asp Glu Leu Tyr Lys
Ter
GFP
AAGCTT junction
M13 rev
GFP reverse (1138 .. 1163)
Colony PCR reverse (1188 .. 1217)
HindIII
1210 1220 1230 1240 1250 1260
TGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAA
ACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTT
1260
lac operator
lac promoter
Colony PCR reverse (1188 .. 1217)
1270 1280 1290 1300 1310 1320
GCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCT
CGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGA
1320
Sequencing reverse (1288 .. 1307)
1330 1340 1350 1360 1370 1380
TTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
AAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCT
1380
1390 1400 1410 1420 1430 1440
GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTC
CCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAG
1440
1450 1460 1470 1480 1490 1500
GTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA
CAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTT
1500
1510 1520 1530 1540 1550 1560
TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGT
AGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCA
1560
1570 1580 1590 1600 1610 1620
AAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA
TTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTT
1620
ori
1630 1640 1650 1660 1670 1680
AATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT
TTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAA
1680
ori
1690 1700 1710 1720 1730 1740
CCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTG
GGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGAC
1740
ori
1750 1760 1770 1780 1790 1800
TCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC
AGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAG
1800
ori
1810 1820 1830 1840 1850 1860
AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCC
TCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGG
1860
ori
1870 1880 1890 1900 1910 1920
GACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTA
CTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAAT
1920
ori
1930 1940 1950 1960 1970 1980
TCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT
AGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGA
1980
ori
1990 2000 2010 2020 2030 2040
ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATC
TGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAG
2040
ori
2050 2060 2070 2080 2090 2100
TGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAA
ACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTT
2100
ori
2110 2120 2130 2140 2150 2160
CAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAA
GTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTT
2160
ori
2170 2180 2190 2200 2210 2220
AAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAA
TTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTT
2220
ori
2230 2240 2250 2260 2270 2280
AACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTT
TTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAA
2280
2290 2300 2310 2320 2330 2340
TTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGAC
AATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTG
2340
2350 2360 2370 2380 2390 2400
AGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCC
TCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGG
2400
Trp His Lys Ile Leu Ser Ala Gly Ile Glu Ala Ile Gln Arg Asn Arg Glu Asp
Ter
AmpR
2410 2420 2430 2440 2450 2460
ATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGC
TATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCG
2460
Met Thr Ala Gln Ser Gly Thr Thr Tyr Ile Val Val Ile Arg Ser Pro Lys Gly Asp Pro
AmpR
2470 2480 2490 2500 2510 2520
CCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATA
GGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTAT
2520
Gly Leu Ala Ala Ile Ile Gly Arg Ser Gly Arg Glu Gly Ala Gly Ser Lys Asp Ala Ile
AmpR
2530 2540 2550 2560 2570 2580
AACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATC
TTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAG
2580
Phe Trp Gly Ala Pro Leu Ala Ser Arg Leu Leu Pro Gly Ala Val Lys Asp Ala Glu Met
AmpR
2590 2600 2610 2620 2630 2640
CAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGC
GTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCG
2640
Trp Asp Ile Leu Gln Gln Arg Ser Ala Leu Thr Leu Leu Glu Gly Thr Leu Leu Lys Arg
AmpR
2650 2660 2670 2680 2690 2700
AACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCA
TTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGT
2700
Leu Thr Thr Ala Met Ala Val Pro Met Thr Thr Asp Arg Glu Asp Asn Pro Ile Ala Glu
AmpR
2710 2720 2730 2740 2750 2760
TTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAA
AAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTT
2760
Asn Leu Glu Pro Glu Trp Arg Asp Leu Arg Thr Val His Asp Gly Met Asn His Leu Phe
AmpR
2770 2780 2790 2800 2810 2820
GCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCA
CGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGT
2820
Ala Thr Leu Glu Lys Pro Gly Gly Ile Thr Thr Leu Leu Leu Asn Ala Ala Thr Asn Asp
AmpR
2830 2840 2850 2860 2870 2880
CTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTT
GAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAA
2880
Ser Met Thr Ile Ala Ala Ser Cys Leu Glu Arg Val Thr Met Gly Asp Thr Leu His Lys
AmpR
2890 2900 2910 2920 2930 2940
TCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGT
AGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCA
2940
Glu Thr Val Pro Ser Tyr Glu Val Leu Asp Asn Gln Ser Tyr His Ile Arg Arg Gly Leu
AmpR
2950 2960 2970 2980 2990 3000
TGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTG
ACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCAC
3000
Gln Glu Gln Gly Ala Asp Ile Arg Ser Leu Val Ala Gly Cys Leu Leu Val Lys Phe Thr
AmpR
3010 3020 3030 3040 3050 3060
CTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGA
GAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCT
3060
Ser Met Met Pro Phe Arg Glu Glu Pro Arg Phe Ser Glu Leu Ile Lys Gly Ser Asn Leu
AmpR
3070 3080 3090 3100 3110 3120
TCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACC
AGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGG
3120
Asp Leu Glu Ile Tyr Gly Val Arg Ala Gly Leu Gln Asp Glu Ala Asp Lys Val Lys Val
AmpR
3130 3140 3150 3160 3170 3180
AGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCG
TCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGC
3180
Leu Thr Glu Pro His Ala Phe Val Pro Leu Cys Phe Ala Ala Phe Phe Pro Ile Leu Ala
AmpR
3190 3200 3210 3220 3230 3240
ACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAG
TGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTC
3240
Val Arg Phe His Gln Ile Ser Met
AmpR
AmpR promoter
3250 3260 3270 3280 3290 3300
GGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
3300
AmpR promoter
3310 3320 3330 3340 3350 3360
GTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATG
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTAC
3360
AmpR promoter
3370 3380 3390 3400
ACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC
TGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAG
3403
10 20 30
TCGCGCGTTTCGGTGATGACGGTGAAAACC
AGCGCGCAAAGCCACTACTGCCACTTTTGG
30
40 50 60
TCTGACACATGCAGCTCCCGGAGACGGTCA
AGACTGTGTACGTCGAGGGCCTCTGCCAGT
60
70 80 90
CAGCTTGTCTGTAAGCGGATGCCGGGAGCA
GTCGAACAGACATTCGCCTACGGCCCTCGT
90
100 110 120
GACAAGCCCGTCAGGGCGCGTCAGCGGGTG
CTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
120
130 140 150
TTGGCGGGTGTCGGGGCTGGCTTAACTATG
AACCGCCCACAGCCCCGACCGAATTGATAC
150
Ile
lacZα
160 170 180
CGGCATCAGAGCAGATTGTACTGAGAGTGC
GCCGTAGTCTCGTCTAACATGACTCTCACG
180
Arg Cys Leu Leu Asn Tyr Gln Ser His
Ter
lacZα
190 200 210
ACCATATGCGGTGTGAAATACCGCACAGAT
TGGTATACGCCACACTTTATGGCGTGTCTA
210
Val Met His Pro Thr Phe Tyr Arg Val Ser
lacZα
220 230 240
GCGTAAGGAGAAAATACCGCATCAGGCGCC
CGCATTCCTCTTTTATGGCGTAGTCCGCGG
240
Ala Tyr Pro Ser Phe Val Ala Asp Pro Ala
lacZα
250 260 270
ATTCGCCATTCAGGCTGCGCAACTGTTGGG
TAAGCGGTAAGTCCGACGCGTTGACAACCC
270
Met Arg Trp Glu Pro Gln Ala Val Thr Pro
lacZα
280 290 300
AAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TTCCCGCTAGCCACGCCCGGAGAAGCGATA
300
Phe Pro Ser Arg His Pro Gly Arg Arg
Ter
lacZα
310 320 330
TACGCCAGCTGGCGAAAGGGGGATGTGCTG
ATGCGGTCGACCGCTTTCCCCCTACACGAC
330
Ala Leu Gln Arg Phe Pro Ser Thr Ser
Ter
lacZα
Sequencing forward (325 .. 341)
340 350 360
CAAGGCGATTAAGTTGGGTAACGCCAGGGT
GTTCCGCTAATTCAACCCATTGCGGTCCCA
360
Cys Pro Ser Thr Pro Tyr Arg Trp Pro
Ter
lacZα
Sequencing forward (325 .. 341)
370 380 390
TTTCCCAGTCACGACGTTGTAAAACGACGG
AAAGGGTCAGTGCTGCAACATTTTGCTGCC
390
Lys Gly Leu Ser Thr Thr Phe Arg Arg
Ter
lacZα
M13 fwd
Colony PCR forward (390 .. 412)
400 410 420
CCAGTGAATTCGAGCTCGGTACCCGGGGAT
GGTCACTTAAGCTCGAGCCATGGGCCCCTA
420
Gly Thr Phe Glu Leu Glu Thr Gly Pro Ser
lacZα
M13 fwd
MCS
Colony PCR forward (390 .. 412)
430 440 450
CCTCTAGAGTCGACCTGCAGGCATGCATGA
GGAGATCTCAGCTGGACGTCCGTACGTACT
450
Gly Arg Ser Asp Val Gln Leu Cys Ser
lacZα
MCS
GCATGC junction
Met
GFP
GFP forward (447 .. 472)
SphI
460 470 480
GTAAAGGAGAAGAACTTTTCACTGGAGTTG
CATTTCCTCTTCTTGAAAAGTGACCTCAAC
480
Ser Lys Gly Glu Glu Leu Phe Thr Gly Val
GFP
GFP forward (447 .. 472)
490 500 510
TCCCAATTCTTGTTGAATTAGATGGTGATG
AGGGTTAAGAACAACTTAATCTACCACTAC
510
Val Pro Ile Leu Val Glu Leu Asp Gly Asp
GFP
520 530 540
TTAATGGGCACAAATTTTCTGTCAGTGGAG
AATTACCCGTGTTTAAAAGACAGTCACCTC
540
Val Asn Gly His Lys Phe Ser Val Ser Gly
GFP
550 560 570
AGGGTGAAGGTGATGCAACATACGGAAAAC
TCCCACTTCCACTACGTTGTATGCCTTTTG
570
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys
GFP
580 590 600
TTACCCTTAAATTTATTTGCACTACTGGAA
AATGGGAATTTAAATAAACGTGATGACCTT
600
Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly
GFP
610 620 630
AACTACCTGTTCCATGGCCAACACTTGTCA
TTGATGGACAAGGTACCGGTTGTGAACAGT
630
Lys Leu Pro Val Pro Trp Pro Thr Leu Val
GFP
640 650 660
CTACTTTCTCTTATGGTGTTCAATGCTTTT
GATGAAAGAGAATACCACAAGTTACGAAAA
660
Thr Thr Phe Ser Tyr Gly Val Gln Cys Phe
GFP
670 680 690
CAAGATACCCAGATCATATGAAACGGCATG
GTTCTATGGGTCTAGTATACTTTGCCGTAC
690
Ser Arg Tyr Pro Asp His Met Lys Arg His
GFP
700 710 720
ACTTTTTCAAGAGTGCCATGCCCGAAGGTT
TGAAAAAGTTCTCACGGTACGGGCTTCCAA
720
Asp Phe Phe Lys Ser Ala Met Pro Glu Gly
GFP
730 740 750
ATGTACAGGAAAGAACTATATTTTTCAAAG
TACATGTCCTTTCTTGATATAAAAAGTTTC
750
Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys
GFP
760 770 780
ATGACGGGAACTACAAGACACGTGCTGAAG
TACTGCCCTTGATGTTCTGTGCACGACTTC
780
Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu
GFP
790 800 810
TCAAGTTTGAAGGTGATACCCTTGTTAATA
AGTTCAAACTTCCACTATGGGAACAATTAT
810
Val Lys Phe Glu Gly Asp Thr Leu Val Asn
GFP
820 830 840
GAATCGAGTTAAAAGGTATTGATTTTAAAG
CTTAGCTCAATTTTCCATAACTAAAATTTC
840
Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys
GFP
850 860 870
AAGATGGAAACATTCTTGGACACAAATTGG
TTCTACCTTTGTAAGAACCTGTGTTTAACC
870
Glu Asp Gly Asn Ile Leu Gly His Lys Leu
GFP
880 890 900
AATACAACTATAACTCACACAATGTATACA
TTATGTTGATATTGAGTGTGTTACATATGT
900
Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr
GFP
910 920 930
TCATGGCAGACAAACAAAAGAATGGAATCA
AGTACCGTCTGTTTGTTTTCTTACCTTAGT
930
Ile Met Ala Asp Lys Gln Lys Asn Gly Ile
GFP
940 950 960
AAGTTAACTTCAAAATTAGACACAACATTG
TTCAATTGAAGTTTTAATCTGTGTTGTAAC
960
Lys Val Asn Phe Lys Ile Arg His Asn Ile
GFP
970 980 990
AAGATGGAAGCGTTCAACTAGCAGACCATT
TTCTACCTTCGCAAGTTGATCGTCTGGTAA
990
Glu Asp Gly Ser Val Gln Leu Ala Asp His
GFP
1000 1010 1020
ATCAACAAAATACTCCAATTGGCGATGGCC
TAGTTGTTTTATGAGGTTAACCGCTACCGG
1020
Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly
GFP
1030 1040 1050
CTGTCCTTTTACCAGACAACCATTACCTGT
GACAGGAAAATGGTCTGTTGGTAATGGACA
1050
Pro Val Leu Leu Pro Asp Asn His Tyr Leu
GFP
1060 1070 1080
CCACACAATCTGCCCTTTCGAAAGATCCCA
GGTGTGTTAGACGGGAAAGCTTTCTAGGGT
1080
Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro
GFP
1090 1100 1110
ACGAAAAGAGAGACCACATGGTCCTTCTTG
TGCTTTTCTCTCTGGTGTACCAGGAAGAAC
1110
Asn Glu Lys Arg Asp His Met Val Leu Leu
GFP
1120 1130 1140
AGTTTGTAACAGCTGCTGGGATTACACATG
TCAAACATTGTCGACGACCCTAATGTGTAC
1140
Glu Phe Val Thr Ala Ala Gly Ile Thr His
GFP
GFP reverse (1138 .. 1163)
1150 1160 1170
GCATGGATGAACTATACAAATAGAAGCTTG
CGTACCTACTTGATATGTTTATCTTCGAAC
1170
Ser Pro
lacZα
MCS
Gly Met Asp Glu Leu Tyr Lys
Ter
GFP
AAGCTT junction
GFP reverse (1138 .. 1163)
HindIII
1180 1190 1200
GCGTAATCATGGTCATAGCTGTTTCCTGTG
CGCATTAGTACCAGTATCGACAAAGGACAC
1200
Thr Ile Met Thr Met
lacZα
M13 rev
Colony PCR reverse (1188 .. 1217)
1210 1220 1230
TGAAATTGTTATCCGCTCACAATTCCACAC
ACTTTAACAATAGGCGAGTGTTAAGGTGTG
1230
lac operator
lac promoter
Colony PCR reverse (1188 .. 1217)
1240 1250 1260
AACATACGAGCCGGAAGCATAAAGTGTAAA
TTGTATGCTCGGCCTTCGTATTTCACATTT
1260
lac promoter
1270 1280 1290
GCCTGGGGTGCCTAATGAGTGAGCTAACTC
CGGACCCCACGGATTACTCACTCGATTGAG
1290
Sequencing reverse (1288 .. 1307)
1300 1310 1320
ACATTAATTGCGTTGCGCTCACTGCCCGCT
TGTAATTAACGCAACGCGAGTGACGGGCGA
1320
Sequencing reverse (1288 .. 1307)
1330 1340 1350
TTCCAGTCGGGAAACCTGTCGTGCCAGCTG
AAGGTCAGCCCTTTGGACAGCACGGTCGAC
1350
1360 1370 1380
CATTAATGAATCGGCCAACGCGCGGGGAGA
GTAATTACTTAGCCGGTTGCGCGCCCCTCT
1380
1390 1400 1410
GGCGGTTTGCGTATTGGGCGCTCTTCCGCT
CCGCCAAACGCATAACCCGCGAGAAGGCGA
1410
1420 1430 1440
TCCTCGCTCACTGACTCGCTGCGCTCGGTC
AGGAGCGAGTGACTGAGCGACGCGAGCCAG
1440
1450 1460 1470
GTTCGGCTGCGGCGAGCGGTATCAGCTCAC
CAAGCCGACGCCGCTCGCCATAGTCGAGTG
1470
1480 1490 1500
TCAAAGGCGGTAATACGGTTATCCACAGAA
AGTTTCCGCCATTATGCCAATAGGTGTCTT
1500
1510 1520 1530
TCAGGGGATAACGCAGGAAAGAACATGTGA
AGTCCCCTATTGCGTCCTTTCTTGTACACT
1530
1540 1550 1560
GCAAAAGGCCAGCAAAAGGCCAGGAACCGT
CGTTTTCCGGTCGTTTTCCGGTCCTTGGCA
1560
1570 1580 1590
AAAAAGGCCGCGTTGCTGGCGTTTTTCCAT
TTTTTCCGGCGCAACGACCGCAAAAAGGTA
1590
ori
1600 1610 1620
AGGCTCCGCCCCCCTGACGAGCATCACAAA
TCCGAGGCGGGGGGACTGCTCGTAGTGTTT
1620
ori
1630 1640 1650
AATCGACGCTCAAGTCAGAGGTGGCGAAAC
TTAGCTGCGAGTTCAGTCTCCACCGCTTTG
1650
ori
1660 1670 1680
CCGACAGGACTATAAAGATACCAGGCGTTT
GGCTGTCCTGATATTTCTATGGTCCGCAAA
1680
ori
1690 1700 1710
CCCCCTGGAAGCTCCCTCGTGCGCTCTCCT
GGGGGACCTTCGAGGGAGCACGCGAGAGGA
1710
ori
1720 1730 1740
GTTCCGACCCTGCCGCTTACCGGATACCTG
CAAGGCTGGGACGGCGAATGGCCTATGGAC
1740
ori
1750 1760 1770
TCCGCCTTTCTCCCTTCGGGAAGCGTGGCG
AGGCGGAAAGAGGGAAGCCCTTCGCACCGC
1770
ori
1780 1790 1800
CTTTCTCATAGCTCACGCTGTAGGTATCTC
GAAAGAGTATCGAGTGCGACATCCATAGAG
1800
ori
1810 1820 1830
AGTTCGGTGTAGGTCGTTCGCTCCAAGCTG
TCAAGCCACATCCAGCAAGCGAGGTTCGAC
1830
ori
1840 1850 1860
GGCTGTGTGCACGAACCCCCCGTTCAGCCC
CCGACACACGTGCTTGGGGGGCAAGTCGGG
1860
ori
1870 1880 1890
GACCGCTGCGCCTTATCCGGTAACTATCGT
CTGGCGACGCGGAATAGGCCATTGATAGCA
1890
ori
1900 1910 1920
CTTGAGTCCAACCCGGTAAGACACGACTTA
GAACTCAGGTTGGGCCATTCTGTGCTGAAT
1920
ori
1930 1940 1950
TCGCCACTGGCAGCAGCCACTGGTAACAGG
AGCGGTGACCGTCGTCGGTGACCATTGTCC
1950
ori
1960 1970 1980
ATTAGCAGAGCGAGGTATGTAGGCGGTGCT
TAATCGTCTCGCTCCATACATCCGCCACGA
1980
ori
1990 2000 2010
ACAGAGTTCTTGAAGTGGTGGCCTAACTAC
TGTCTCAAGAACTTCACCACCGGATTGATG
2010
ori
2020 2030 2040
GGCTACACTAGAAGAACAGTATTTGGTATC
CCGATGTGATCTTCTTGTCATAAACCATAG
2040
ori
2050 2060 2070
TGCGCTCTGCTGAAGCCAGTTACCTTCGGA
ACGCGAGACGACTTCGGTCAATGGAAGCCT
2070
ori
2080 2090 2100
AAAAGAGTTGGTAGCTCTTGATCCGGCAAA
TTTTCTCAACCATCGAGAACTAGGCCGTTT
2100
ori
2110 2120 2130
CAAACCACCGCTGGTAGCGGTGGTTTTTTT
GTTTGGTGGCGACCATCGCCACCAAAAAAA
2130
ori
2140 2150 2160
GTTTGCAAGCAGCAGATTACGCGCAGAAAA
CAAACGTTCGTCGTCTAATGCGCGTCTTTT
2160
ori
2170 2180 2190
AAAGGATCTCAAGAAGATCCTTTGATCTTT
TTTCCTAGAGTTCTTCTAGGAAACTAGAAA
2190
ori
2200 2210 2220
TCTACGGGGTCTGACGCTCAGTGGAACGAA
AGATGCCCCAGACTGCGAGTCACCTTGCTT
2220
2230 2240 2250
AACTCACGTTAAGGGATTTTGGTCATGAGA
TTGAGTGCAATTCCCTAAAACCAGTACTCT
2250
2260 2270 2280
TTATCAAAAAGGATCTTCACCTAGATCCTT
AATAGTTTTTCCTAGAAGTGGATCTAGGAA
2280
2290 2300 2310
TTAAATTAAAAATGAAGTTTTAAATCAATC
AATTTAATTTTTACTTCAAAATTTAGTTAG
2310
2320 2330 2340
TAAAGTATATATGAGTAAACTTGGTCTGAC
ATTTCATATATACTCATTTGAACCAGACTG
2340
2350 2360 2370
AGTTACCAATGCTTAATCAGTGAGGCACCT
TCAATGGTTACGAATTAGTCACTCCGTGGA
2370
Trp His Lys Ile Leu Ser Ala Gly
Ter
AmpR
2380 2390 2400
ATCTCAGCGATCTGTCTATTTCGTTCATCC
TAGAGTCGCTAGACAGATAAAGCAAGTAGG
2400
Ile Glu Ala Ile Gln Arg Asn Arg Glu Asp
AmpR
2410 2420 2430
ATAGTTGCCTGACTCCCCGTCGTGTAGATA
TATCAACGGACTGAGGGGCAGCACATCTAT
2430
Met Thr Ala Gln Ser Gly Thr Thr Tyr Ile
AmpR
2440 2450 2460
ACTACGATACGGGAGGGCTTACCATCTGGC
TGATGCTATGCCCTCCCGAATGGTAGACCG
2460
Val Val Ile Arg Ser Pro Lys Gly Asp Pro
AmpR
2470 2480 2490
CCCAGTGCTGCAATGATACCGCGAGACCCA
GGGTCACGACGTTACTATGGCGCTCTGGGT
2490
Gly Leu Ala Ala Ile Ile Gly Arg Ser Gly
AmpR
2500 2510 2520
CGCTCACCGGCTCCAGATTTATCAGCAATA
GCGAGTGGCCGAGGTCTAAATAGTCGTTAT
2520
Arg Glu Gly Ala Gly Ser Lys Asp Ala Ile
AmpR
2530 2540 2550
AACCAGCCAGCCGGAAGGGCCGAGCGCAGA
TTGGTCGGTCGGCCTTCCCGGCTCGCGTCT
2550
Phe Trp Gly Ala Pro Leu Ala Ser Arg Leu
AmpR
2560 2570 2580
AGTGGTCCTGCAACTTTATCCGCCTCCATC
TCACCAGGACGTTGAAATAGGCGGAGGTAG
2580
Leu Pro Gly Ala Val Lys Asp Ala Glu Met
AmpR
2590 2600 2610
CAGTCTATTAATTGTTGCCGGGAAGCTAGA
GTCAGATAATTAACAACGGCCCTTCGATCT
2610
Trp Asp Ile Leu Gln Gln Arg Ser Ala Leu
AmpR
2620 2630 2640
GTAAGTAGTTCGCCAGTTAATAGTTTGCGC
CATTCATCAAGCGGTCAATTATCAAACGCG
2640
Thr Leu Leu Glu Gly Thr Leu Leu Lys Arg
AmpR
2650 2660 2670
AACGTTGTTGCCATTGCTACAGGCATCGTG
TTGCAACAACGGTAACGATGTCCGTAGCAC
2670
Leu Thr Thr Ala Met Ala Val Pro Met Thr
AmpR
2680 2690 2700
GTGTCACGCTCGTCGTTTGGTATGGCTTCA
CACAGTGCGAGCAGCAAACCATACCGAAGT
2700
Thr Asp Arg Glu Asp Asn Pro Ile Ala Glu
AmpR
2710 2720 2730
TTCAGCTCCGGTTCCCAACGATCAAGGCGA
AAGTCGAGGCCAAGGGTTGCTAGTTCCGCT
2730
Asn Leu Glu Pro Glu Trp Arg Asp Leu Arg
AmpR
2740 2750 2760
GTTACATGATCCCCCATGTTGTGCAAAAAA
CAATGTACTAGGGGGTACAACACGTTTTTT
2760
Thr Val His Asp Gly Met Asn His Leu Phe
AmpR
2770 2780 2790
GCGGTTAGCTCCTTCGGTCCTCCGATCGTT
CGCCAATCGAGGAAGCCAGGAGGCTAGCAA
2790
Ala Thr Leu Glu Lys Pro Gly Gly Ile Thr
AmpR
2800 2810 2820
GTCAGAAGTAAGTTGGCCGCAGTGTTATCA
CAGTCTTCATTCAACCGGCGTCACAATAGT
2820
Thr Leu Leu Leu Asn Ala Ala Thr Asn Asp
AmpR
2830 2840 2850
CTCATGGTTATGGCAGCACTGCATAATTCT
GAGTACCAATACCGTCGTGACGTATTAAGA
2850
Ser Met Thr Ile Ala Ala Ser Cys Leu Glu
AmpR
2860 2870 2880
CTTACTGTCATGCCATCCGTAAGATGCTTT
GAATGACAGTACGGTAGGCATTCTACGAAA
2880
Arg Val Thr Met Gly Asp Thr Leu His Lys
AmpR
2890 2900 2910
TCTGTGACTGGTGAGTACTCAACCAAGTCA
AGACACTGACCACTCATGAGTTGGTTCAGT
2910
Glu Thr Val Pro Ser Tyr Glu Val Leu Asp
AmpR
2920 2930 2940
TTCTGAGAATAGTGTATGCGGCGACCGAGT
AAGACTCTTATCACATACGCCGCTGGCTCA
2940
Asn Gln Ser Tyr His Ile Arg Arg Gly Leu
AmpR
2950 2960 2970
TGCTCTTGCCCGGCGTCAATACGGGATAAT
ACGAGAACGGGCCGCAGTTATGCCCTATTA
2970
Gln Glu Gln Gly Ala Asp Ile Arg Ser Leu
AmpR
2980 2990 3000
ACCGCGCCACATAGCAGAACTTTAAAAGTG
TGGCGCGGTGTATCGTCTTGAAATTTTCAC
3000
Val Ala Gly Cys Leu Leu Val Lys Phe Thr
AmpR
3010 3020 3030
CTCATCATTGGAAAACGTTCTTCGGGGCGA
GAGTAGTAACCTTTTGCAAGAAGCCCCGCT
3030
Ser Met Met Pro Phe Arg Glu Glu Pro Arg
AmpR
3040 3050 3060
AAACTCTCAAGGATCTTACCGCTGTTGAGA
TTTGAGAGTTCCTAGAATGGCGACAACTCT
3060
Phe Ser Glu Leu Ile Lys Gly Ser Asn Leu
AmpR
3070 3080 3090
TCCAGTTCGATGTAACCCACTCGTGCACCC
AGGTCAAGCTACATTGGGTGAGCACGTGGG
3090
Asp Leu Glu Ile Tyr Gly Val Arg Ala Gly
AmpR
3100 3110 3120
AACTGATCTTCAGCATCTTTTACTTTCACC
TTGACTAGAAGTCGTAGAAAATGAAAGTGG
3120
Leu Gln Asp Glu Ala Asp Lys Val Lys Val
AmpR
3130 3140 3150
AGCGTTTCTGGGTGAGCAAAAACAGGAAGG
TCGCAAAGACCCACTCGTTTTTGTCCTTCC
3150
Leu Thr Glu Pro His Ala Phe Val Pro Leu
AmpR
3160 3170 3180
CAAAATGCCGCAAAAAAGGGAATAAGGGCG
GTTTTACGGCGTTTTTTCCCTTATTCCCGC
3180
Cys Phe Ala Ala Phe Phe Pro Ile Leu Ala
AmpR
3190 3200 3210
ACACGGAAATGTTGAATACTCATACTCTTC
TGTGCCTTTACAACTTATGAGTATGAGAAG
3210
Val Arg Phe His Gln Ile Ser Met
AmpR
AmpR promoter
3220 3230 3240
CTTTTTCAATATTATTGAAGCATTTATCAG
GAAAAAGTTATAATAACTTCGTAAATAGTC
3240
AmpR promoter
3250 3260 3270
GGTTATTGTCTCATGAGCGGATACATATTT
CCAATAACAGAGTACTCGCCTATGTATAAA
3270
AmpR promoter
3280 3290 3300
GAATGTATTTAGAAAAATAAACAAATAGGG
CTTACATAAATCTTTTTATTTGTTTATCCC
3300
AmpR promoter
3310 3320 3330
GTTCCGCGCACATTTCCCCGAAAAGTGCCA
CAAGGCGCGTGTAAAGGGGCTTTTCACGGT
3330
AmpR promoter
3340 3350 3360
CCTGACGTCTAAGAAACCATTATTATCATG
GGACTGCAGATTCTTTGGTAATAATAGTAC
3360
3370 3380 3390
ACATTAACCTATAAAAATAGGCGTATCACG
TGTAATTGGATATTTTTATCCGCATAGTGC
3390
3400
AGGCCCTTTCGTC
TCCGGGAAAGCAG
3403