name	sequence	length	tm_c
GFP forward	AAACACGCATGCATGAGTAAAGGAGAAGAACTTTTCAC	38	62.1
GFP reverse	AAACACAAGCTTCTATTTGTATAGTTCATCCATGCCAT	38	62.0
Colony PCR forward	GCCAGTGAATTCGAGCTCGGTAC	23	61.9
Colony PCR reverse	AGCGGATAACAATTTCACACAGGAAACAGC	30	62.1
Sequencing forward	GTGCTGCAAGGCGATTA	17	62.8
Sequencing reverse	CGCAACGCAATTAATGTGAG	20	62.2