Features
Primers
Cut sites
CDS
misc_feature
primer_bind
promoter
protein_bind
rep_origin
Sequence
Zoom
Reset
500
1000
1500
2000
2500
M13 fwd
AmpR promoter
lacZα
ori
AmpR
TGGC overhang
BbsI site
ATGA overhang
lac operator
M13 rev
lac promoter
MCS
BbsI site
pUC19 backbone forward (19 .. 42)
pUC19 backbone reverse (2627 .. 2644)
BbsI
(14)
BbsI
(2644)
pUC19 backbone
2662 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
MCS
AmpR promoter
lacZα
ori
AmpR
TGGC overhang
ATGA overhang
lac operator
M13 rev
M13 fwd
lac promoter
BbsI site
BbsI site
pUC19 backbone forward (19 .. 42)
pUC19 backbone reverse (2627 .. 2644)
BbsI
(14)
BbsI
(2644)
pUC19 backbone
2662 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
MCS
AmpR promoter
lacZα
ori
AmpR
TGGC overhang
ATGA overhang
lac operator
M13 rev
M13 fwd
lac promoter
BbsI site
BbsI site
pUC19 backbone forward (19 .. 42)
pUC19 backbone reverse (2627 .. 2644)
BbsI
(14)
BbsI
(2644)
pUC19 backbone
2662 bp
50
100
150
200
250
300
350
400
450
500
550
600
650
700
750
800
850
900
950
1000
1050
1100
1150
1200
1250
1300
1350
1400
1450
1500
1550
1600
1650
1700
1750
1800
1850
1900
1950
2000
2050
2100
2150
2200
2250
2300
2350
2400
2450
2500
2550
2600
2650
M13 rev
M13 fwd
lac promoter
AmpR promoter
lacZα
ori
AmpR
TGGC overhang
ATGA overhang
lac operator
MCS
BbsI site
BbsI site
pUC19 backbone forward (19 .. 42)
pUC19 backbone reverse (2627 .. 2644)
BbsI
(14)
BbsI
(2644)
pUC19 backbone
2662 bp
Both strands
Copy
10 20 30 40 50 60
AAACACGAAGACAATGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCC
TTTGTGCTTCTGTTACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGG
60
BbsI site
MCS
TGGC overhang
M13 rev
lac operator
pUC19 backbone forward (19 .. 42)
BbsI
70 80 90 100 110 120
GCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTA
CGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGAT
120
lac operator
lac promoter
130 140 150 160 170 180
ATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAA
TACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTT
180
190 200 210 220 230 240
CCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT
GGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATA
240
250 260 270 280 290 300
TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
ACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGC
300
310 320 330 340 350 360
AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC
TCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCG
360
370 380 390 400 410 420
AGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT
TCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAA
420
430 440 450 460 470 480
GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAG
CGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTC
480
ori
490 500 510 520 530 540
TCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC
AGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAG
540
ori
550 560 570 580 590 600
CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC
GGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGG
600
ori
610 620 630 640 650 660
TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT
AAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCA
660
ori
670 680 690 700 710 720
CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT
GCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAA
720
ori
730 740 750 760 770 780
ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGC
TAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCG
780
ori
790 800 810 820 830 840
AGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
TCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTT
840
ori
850 860 870 880 890 900
GTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAA
CACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTT
900
ori
910 920 930 940 950 960
GCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG
CGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACC
960
ori
970 980 990 1000 1010 1020
TAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA
ATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCT
1020
ori
1030 1040 1050 1060 1070 1080
AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGG
TCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCC
1080
1090 1100 1110 1120 1130 1140
GATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG
CTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTAC
1140
1150 1160 1170 1180 1190 1200
AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTT
TTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAA
1200
Trp His Lys
Ter
AmpR
1210 1220 1230 1240 1250 1260
AATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACT
TTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGA
1260
Ile Leu Ser Ala Gly Ile Glu Ala Ile Gln Arg Asn Arg Glu Asp Met Thr Ala Gln Ser
AmpR
1270 1280 1290 1300 1310 1320
CCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAAT
GGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTA
1320
Gly Thr Thr Tyr Ile Val Val Ile Arg Ser Pro Lys Gly Asp Pro Gly Leu Ala Ala Ile
AmpR
1330 1340 1350 1360 1370 1380
GATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGG
CTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCC
1380
Ile Gly Arg Ser Gly Arg Glu Gly Ala Gly Ser Lys Asp Ala Ile Phe Trp Gly Ala Pro
AmpR
1390 1400 1410 1420 1430 1440
AAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG
TTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAAC
1440
Leu Ala Ser Arg Leu Leu Pro Gly Ala Val Lys Asp Ala Glu Met Trp Asp Ile Leu Gln
AmpR
1450 1460 1470 1480 1490 1500
TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCAT
AACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTA
1500
Gln Arg Ser Ala Leu Thr Leu Leu Glu Gly Thr Leu Leu Lys Arg Leu Thr Thr Ala Met
AmpR
1510 1520 1530 1540 1550 1560
TGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTC
ACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAG
1560
Ala Val Pro Met Thr Thr Asp Arg Glu Asp Asn Pro Ile Ala Glu Asn Leu Glu Pro Glu
AmpR
1570 1580 1590 1600 1610 1620
CCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTT
GGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAA
1620
Trp Arg Asp Leu Arg Thr Val His Asp Gly Met Asn His Leu Phe Ala Thr Leu Glu Lys
AmpR
1630 1640 1650 1660 1670 1680
CGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGC
GCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCG
1680
Pro Gly Gly Ile Thr Thr Leu Leu Leu Asn Ala Ala Thr Asn Asp Ser Met Thr Ile Ala
AmpR
1690 1700 1710 1720 1730 1740
AGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA
TCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACT
1740
Ala Ser Cys Leu Glu Arg Val Thr Met Gly Asp Thr Leu His Lys Glu Thr Val Pro Ser
AmpR
1750 1760 1770 1780 1790 1800
GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGC
CATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCG
1800
Tyr Glu Val Leu Asp Asn Gln Ser Tyr His Ile Arg Arg Gly Leu Gln Glu Gln Gly Ala
AmpR
1810 1820 1830 1840 1850 1860
GTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAA
CAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTT
1860
Asp Ile Arg Ser Leu Val Ala Gly Cys Leu Leu Val Lys Phe Thr Ser Met Met Pro Phe
AmpR
1870 1880 1890 1900 1910 1920
ACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTA
TGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACAT
1920
Arg Glu Glu Pro Arg Phe Ser Glu Leu Ile Lys Gly Ser Asn Leu Asp Leu Glu Ile Tyr
AmpR
1930 1940 1950 1960 1970 1980
ACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTG
TGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCAC
1980
Gly Val Arg Ala Gly Leu Gln Asp Glu Ala Asp Lys Val Lys Val Leu Thr Glu Pro His
AmpR
1990 2000 2010 2020 2030 2040
AGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG
TCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAAC
2040
Ala Phe Val Pro Leu Cys Phe Ala Ala Phe Phe Pro Ile Leu Ala Val Arg Phe His Gln
AmpR
2050 2060 2070 2080 2090 2100
AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCAT
TTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTA
2100
Ile Ser Met
AmpR
AmpR promoter
2110 2120 2130 2140 2150 2160
GAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATT
CTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAA
2160
AmpR promoter
2170 2180 2190 2200 2210 2220
TCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAA
AGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATT
2220
2230 2240 2250 2260 2270 2280
AAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCT
TTTATCCGCATAGTGCTCCGGGAAAGCAGAGCGCGCAAAGCCACTACTGCCACTTTTGGA
2280
2290 2300 2310 2320 2330 2340
CTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAG
GACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTC
2340
2350 2360 2370 2380 2390 2400
ACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGC
TGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGACCGAATTGATACG
2400
Ile Arg
lacZα
2410 2420 2430 2440 2450 2460
GGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATG
CCGTAGTCTCGTCTAACATGACTCTCACGTGGTATACGCCACACTTTATGGCGTGTCTAC
2460
Cys Leu Leu Asn Tyr Gln Ser His Val Met His Pro Thr Phe Tyr Arg Val Ser Ala
Ter
lacZα
2470 2480 2490 2500 2510 2520
CGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGA
GCATTCCTCTTTTATGGCGTAGTCCGCGGTAAGCGGTAAGTCCGACGCGTTGACAACCCT
2520
Tyr Pro Ser Phe Val Ala Asp Pro Ala Met Arg Trp Glu Pro Gln Ala Val Thr Pro Phe
lacZα
2530 2540 2550 2560 2570 2580
AGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGC
TCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGACCGCTTTCCCCCTACACGACG
2580
Pro Ser Arg His Pro Gly Arg Arg Ala Leu Gln Arg Phe Pro Ser Thr Ser Cys
Ter Ter
lacZα
2590 2600 2610 2620 2630 2640
AAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGC
TTCCGCTAATTCAACCCATTGCGGTCCCAAAAGGGTCAGTGCTGCAACATTTTGCTGCCG
2640
Pro Ser Thr Pro Tyr Arg Trp Pro Lys Gly Leu Ser Thr Thr Phe Arg Arg Gly
Ter Ter
lacZα
M13 fwd
pUC19 backbone reverse (2627 .. 2644)
2650 2660
CAGTATGATTGTCTTCGTGTTT
GTCATACTAACAGAAGCACAAA
2662
Thr
lacZα
M13 fwd
ATGA overhang
BbsI site
pUC19 backbone reverse (2627 .. 2644)
BbsI
10 20 30
AAACACGAAGACAATGGCGTAATCATGGTC
TTTGTGCTTCTGTTACCGCATTAGTACCAG
30
BbsI site
MCS
TGGC overhang
M13 rev
pUC19 backbone forward (19 .. 42)
BbsI
40 50 60
ATAGCTGTTTCCTGTGTGAAATTGTTATCC
TATCGACAAAGGACACACTTTAACAATAGG
60
M13 rev
lac operator
pUC19 backbone forward (19 .. 42)
70 80 90
GCTCACAATTCCACACAACATACGAGCCGG
CGAGTGTTAAGGTGTGTTGTATGCTCGGCC
90
lac operator
lac promoter
100 110 120
AAGCATAAAGTGTAAAGCCTGGGGTGCCTA
TTCGTATTTCACATTTCGGACCCCACGGAT
120
lac promoter
130 140 150
ATGAGTGAGCTAACTCACATTAATTGCGTT
TACTCACTCGATTGAGTGTAATTAACGCAA
150
160 170 180
GCGCTCACTGCCCGCTTTCCAGTCGGGAAA
CGCGAGTGACGGGCGAAAGGTCAGCCCTTT
180
190 200 210
CCTGTCGTGCCAGCTGCATTAATGAATCGG
GGACAGCACGGTCGACGTAATTACTTAGCC
210
220 230 240
CCAACGCGCGGGGAGAGGCGGTTTGCGTAT
GGTTGCGCGCCCCTCTCCGCCAAACGCATA
240
250 260 270
TGGGCGCTCTTCCGCTTCCTCGCTCACTGA
ACCCGCGAGAAGGCGAAGGAGCGAGTGACT
270
280 290 300
CTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
GAGCGACGCGAGCCAGCAAGCCGACGCCGC
300
310 320 330
AGCGGTATCAGCTCACTCAAAGGCGGTAAT
TCGCCATAGTCGAGTGAGTTTCCGCCATTA
330
340 350 360
ACGGTTATCCACAGAATCAGGGGATAACGC
TGCCAATAGGTGTCTTAGTCCCCTATTGCG
360
370 380 390
AGGAAAGAACATGTGAGCAAAAGGCCAGCA
TCCTTTCTTGTACACTCGTTTTCCGGTCGT
390
400 410 420
AAAGGCCAGGAACCGTAAAAAGGCCGCGTT
TTTCCGGTCCTTGGCATTTTTCCGGCGCAA
420
430 440 450
GCTGGCGTTTTTCCATAGGCTCCGCCCCCC
CGACCGCAAAAAGGTATCCGAGGCGGGGGG
450
ori
460 470 480
TGACGAGCATCACAAAAATCGACGCTCAAG
ACTGCTCGTAGTGTTTTTAGCTGCGAGTTC
480
ori
490 500 510
TCAGAGGTGGCGAAACCCGACAGGACTATA
AGTCTCCACCGCTTTGGGCTGTCCTGATAT
510
ori
520 530 540
AAGATACCAGGCGTTTCCCCCTGGAAGCTC
TTCTATGGTCCGCAAAGGGGGACCTTCGAG
540
ori
550 560 570
CCTCGTGCGCTCTCCTGTTCCGACCCTGCC
GGAGCACGCGAGAGGACAAGGCTGGGACGG
570
ori
580 590 600
GCTTACCGGATACCTGTCCGCCTTTCTCCC
CGAATGGCCTATGGACAGGCGGAAAGAGGG
600
ori
610 620 630
TTCGGGAAGCGTGGCGCTTTCTCATAGCTC
AAGCCCTTCGCACCGCGAAAGAGTATCGAG
630
ori
640 650 660
ACGCTGTAGGTATCTCAGTTCGGTGTAGGT
TGCGACATCCATAGAGTCAAGCCACATCCA
660
ori
670 680 690
CGTTCGCTCCAAGCTGGGCTGTGTGCACGA
GCAAGCGAGGTTCGACCCGACACACGTGCT
690
ori
700 710 720
ACCCCCCGTTCAGCCCGACCGCTGCGCCTT
TGGGGGGCAAGTCGGGCTGGCGACGCGGAA
720
ori
730 740 750
ATCCGGTAACTATCGTCTTGAGTCCAACCC
TAGGCCATTGATAGCAGAACTCAGGTTGGG
750
ori
760 770 780
GGTAAGACACGACTTATCGCCACTGGCAGC
CCATTCTGTGCTGAATAGCGGTGACCGTCG
780
ori
790 800 810
AGCCACTGGTAACAGGATTAGCAGAGCGAG
TCGGTGACCATTGTCCTAATCGTCTCGCTC
810
ori
820 830 840
GTATGTAGGCGGTGCTACAGAGTTCTTGAA
CATACATCCGCCACGATGTCTCAAGAACTT
840
ori
850 860 870
GTGGTGGCCTAACTACGGCTACACTAGAAG
CACCACCGGATTGATGCCGATGTGATCTTC
870
ori
880 890 900
AACAGTATTTGGTATCTGCGCTCTGCTGAA
TTGTCATAAACCATAGACGCGAGACGACTT
900
ori
910 920 930
GCCAGTTACCTTCGGAAAAAGAGTTGGTAG
CGGTCAATGGAAGCCTTTTTCTCAACCATC
930
ori
940 950 960
CTCTTGATCCGGCAAACAAACCACCGCTGG
GAGAACTAGGCCGTTTGTTTGGTGGCGACC
960
ori
970 980 990
TAGCGGTGGTTTTTTTGTTTGCAAGCAGCA
ATCGCCACCAAAAAAACAAACGTTCGTCGT
990
ori
1000 1010 1020
GATTACGCGCAGAAAAAAAGGATCTCAAGA
CTAATGCGCGTCTTTTTTTCCTAGAGTTCT
1020
ori
1030 1040 1050
AGATCCTTTGATCTTTTCTACGGGGTCTGA
TCTAGGAAACTAGAAAAGATGCCCCAGACT
1050
1060 1070 1080
CGCTCAGTGGAACGAAAACTCACGTTAAGG
GCGAGTCACCTTGCTTTTGAGTGCAATTCC
1080
1090 1100 1110
GATTTTGGTCATGAGATTATCAAAAAGGAT
CTAAAACCAGTACTCTAATAGTTTTTCCTA
1110
1120 1130 1140
CTTCACCTAGATCCTTTTAAATTAAAAATG
GAAGTGGATCTAGGAAAATTTAATTTTTAC
1140
1150 1160 1170
AAGTTTTAAATCAATCTAAAGTATATATGA
TTCAAAATTTAGTTAGATTTCATATATACT
1170
1180 1190 1200
GTAAACTTGGTCTGACAGTTACCAATGCTT
CATTTGAACCAGACTGTCAATGGTTACGAA
1200
Trp His Lys
Ter
AmpR
1210 1220 1230
AATCAGTGAGGCACCTATCTCAGCGATCTG
TTAGTCACTCCGTGGATAGAGTCGCTAGAC
1230
Ile Leu Ser Ala Gly Ile Glu Ala Ile Gln
AmpR
1240 1250 1260
TCTATTTCGTTCATCCATAGTTGCCTGACT
AGATAAAGCAAGTAGGTATCAACGGACTGA
1260
Arg Asn Arg Glu Asp Met Thr Ala Gln Ser
AmpR
1270 1280 1290
CCCCGTCGTGTAGATAACTACGATACGGGA
GGGGCAGCACATCTATTGATGCTATGCCCT
1290
Gly Thr Thr Tyr Ile Val Val Ile Arg Ser
AmpR
1300 1310 1320
GGGCTTACCATCTGGCCCCAGTGCTGCAAT
CCCGAATGGTAGACCGGGGTCACGACGTTA
1320
Pro Lys Gly Asp Pro Gly Leu Ala Ala Ile
AmpR
1330 1340 1350
GATACCGCGAGACCCACGCTCACCGGCTCC
CTATGGCGCTCTGGGTGCGAGTGGCCGAGG
1350
Ile Gly Arg Ser Gly Arg Glu Gly Ala Gly
AmpR
1360 1370 1380
AGATTTATCAGCAATAAACCAGCCAGCCGG
TCTAAATAGTCGTTATTTGGTCGGTCGGCC
1380
Ser Lys Asp Ala Ile Phe Trp Gly Ala Pro
AmpR
1390 1400 1410
AAGGGCCGAGCGCAGAAGTGGTCCTGCAAC
TTCCCGGCTCGCGTCTTCACCAGGACGTTG
1410
Leu Ala Ser Arg Leu Leu Pro Gly Ala Val
AmpR
1420 1430 1440
TTTATCCGCCTCCATCCAGTCTATTAATTG
AAATAGGCGGAGGTAGGTCAGATAATTAAC
1440
Lys Asp Ala Glu Met Trp Asp Ile Leu Gln
AmpR
1450 1460 1470
TTGCCGGGAAGCTAGAGTAAGTAGTTCGCC
AACGGCCCTTCGATCTCATTCATCAAGCGG
1470
Gln Arg Ser Ala Leu Thr Leu Leu Glu Gly
AmpR
1480 1490 1500
AGTTAATAGTTTGCGCAACGTTGTTGCCAT
TCAATTATCAAACGCGTTGCAACAACGGTA
1500
Thr Leu Leu Lys Arg Leu Thr Thr Ala Met
AmpR
1510 1520 1530
TGCTACAGGCATCGTGGTGTCACGCTCGTC
ACGATGTCCGTAGCACCACAGTGCGAGCAG
1530
Ala Val Pro Met Thr Thr Asp Arg Glu Asp
AmpR
1540 1550 1560
GTTTGGTATGGCTTCATTCAGCTCCGGTTC
CAAACCATACCGAAGTAAGTCGAGGCCAAG
1560
Asn Pro Ile Ala Glu Asn Leu Glu Pro Glu
AmpR
1570 1580 1590
CCAACGATCAAGGCGAGTTACATGATCCCC
GGTTGCTAGTTCCGCTCAATGTACTAGGGG
1590
Trp Arg Asp Leu Arg Thr Val His Asp Gly
AmpR
1600 1610 1620
CATGTTGTGCAAAAAAGCGGTTAGCTCCTT
GTACAACACGTTTTTTCGCCAATCGAGGAA
1620
Met Asn His Leu Phe Ala Thr Leu Glu Lys
AmpR
1630 1640 1650
CGGTCCTCCGATCGTTGTCAGAAGTAAGTT
GCCAGGAGGCTAGCAACAGTCTTCATTCAA
1650
Pro Gly Gly Ile Thr Thr Leu Leu Leu Asn
AmpR
1660 1670 1680
GGCCGCAGTGTTATCACTCATGGTTATGGC
CCGGCGTCACAATAGTGAGTACCAATACCG
1680
Ala Ala Thr Asn Asp Ser Met Thr Ile Ala
AmpR
1690 1700 1710
AGCACTGCATAATTCTCTTACTGTCATGCC
TCGTGACGTATTAAGAGAATGACAGTACGG
1710
Ala Ser Cys Leu Glu Arg Val Thr Met Gly
AmpR
1720 1730 1740
ATCCGTAAGATGCTTTTCTGTGACTGGTGA
TAGGCATTCTACGAAAAGACACTGACCACT
1740
Asp Thr Leu His Lys Glu Thr Val Pro Ser
AmpR
1750 1760 1770
GTACTCAACCAAGTCATTCTGAGAATAGTG
CATGAGTTGGTTCAGTAAGACTCTTATCAC
1770
Tyr Glu Val Leu Asp Asn Gln Ser Tyr His
AmpR
1780 1790 1800
TATGCGGCGACCGAGTTGCTCTTGCCCGGC
ATACGCCGCTGGCTCAACGAGAACGGGCCG
1800
Ile Arg Arg Gly Leu Gln Glu Gln Gly Ala
AmpR
1810 1820 1830
GTCAATACGGGATAATACCGCGCCACATAG
CAGTTATGCCCTATTATGGCGCGGTGTATC
1830
Asp Ile Arg Ser Leu Val Ala Gly Cys Leu
AmpR
1840 1850 1860
CAGAACTTTAAAAGTGCTCATCATTGGAAA
GTCTTGAAATTTTCACGAGTAGTAACCTTT
1860
Leu Val Lys Phe Thr Ser Met Met Pro Phe
AmpR
1870 1880 1890
ACGTTCTTCGGGGCGAAAACTCTCAAGGAT
TGCAAGAAGCCCCGCTTTTGAGAGTTCCTA
1890
Arg Glu Glu Pro Arg Phe Ser Glu Leu Ile
AmpR
1900 1910 1920
CTTACCGCTGTTGAGATCCAGTTCGATGTA
GAATGGCGACAACTCTAGGTCAAGCTACAT
1920
Lys Gly Ser Asn Leu Asp Leu Glu Ile Tyr
AmpR
1930 1940 1950
ACCCACTCGTGCACCCAACTGATCTTCAGC
TGGGTGAGCACGTGGGTTGACTAGAAGTCG
1950
Gly Val Arg Ala Gly Leu Gln Asp Glu Ala
AmpR
1960 1970 1980
ATCTTTTACTTTCACCAGCGTTTCTGGGTG
TAGAAAATGAAAGTGGTCGCAAAGACCCAC
1980
Asp Lys Val Lys Val Leu Thr Glu Pro His
AmpR
1990 2000 2010
AGCAAAAACAGGAAGGCAAAATGCCGCAAA
TCGTTTTTGTCCTTCCGTTTTACGGCGTTT
2010
Ala Phe Val Pro Leu Cys Phe Ala Ala Phe
AmpR
2020 2030 2040
AAAGGGAATAAGGGCGACACGGAAATGTTG
TTTCCCTTATTCCCGCTGTGCCTTTACAAC
2040
Phe Pro Ile Leu Ala Val Arg Phe His Gln
AmpR
2050 2060 2070
AATACTCATACTCTTCCTTTTTCAATATTA
TTATGAGTATGAGAAGGAAAAAGTTATAAT
2070
Ile Ser Met
AmpR
AmpR promoter
2080 2090 2100
TTGAAGCATTTATCAGGGTTATTGTCTCAT
AACTTCGTAAATAGTCCCAATAACAGAGTA
2100
AmpR promoter
2110 2120 2130
GAGCGGATACATATTTGAATGTATTTAGAA
CTCGCCTATGTATAAACTTACATAAATCTT
2130
AmpR promoter
2140 2150 2160
AAATAAACAAATAGGGGTTCCGCGCACATT
TTTATTTGTTTATCCCCAAGGCGCGTGTAA
2160
AmpR promoter
2170 2180 2190
TCCCCGAAAAGTGCCACCTGACGTCTAAGA
AGGGGCTTTTCACGGTGGACTGCAGATTCT
2190
2200 2210 2220
AACCATTATTATCATGACATTAACCTATAA
TTGGTAATAATAGTACTGTAATTGGATATT
2220
2230 2240 2250
AAATAGGCGTATCACGAGGCCCTTTCGTCT
TTTATCCGCATAGTGCTCCGGGAAAGCAGA
2250
2260 2270 2280
CGCGCGTTTCGGTGATGACGGTGAAAACCT
GCGCGCAAAGCCACTACTGCCACTTTTGGA
2280
2290 2300 2310
CTGACACATGCAGCTCCCGGAGACGGTCAC
GACTGTGTACGTCGAGGGCCTCTGCCAGTG
2310
2320 2330 2340
AGCTTGTCTGTAAGCGGATGCCGGGAGCAG
TCGAACAGACATTCGCCTACGGCCCTCGTC
2340
2350 2360 2370
ACAAGCCCGTCAGGGCGCGTCAGCGGGTGT
TGTTCGGGCAGTCCCGCGCAGTCGCCCACA
2370
2380 2390 2400
TGGCGGGTGTCGGGGCTGGCTTAACTATGC
ACCGCCCACAGCCCCGACCGAATTGATACG
2400
Ile Arg
lacZα
2410 2420 2430
GGCATCAGAGCAGATTGTACTGAGAGTGCA
CCGTAGTCTCGTCTAACATGACTCTCACGT
2430
Cys Leu Leu Asn Tyr Gln Ser His Val
Ter
lacZα
2440 2450 2460
CCATATGCGGTGTGAAATACCGCACAGATG
GGTATACGCCACACTTTATGGCGTGTCTAC
2460
Met His Pro Thr Phe Tyr Arg Val Ser Ala
lacZα
2470 2480 2490
CGTAAGGAGAAAATACCGCATCAGGCGCCA
GCATTCCTCTTTTATGGCGTAGTCCGCGGT
2490
Tyr Pro Ser Phe Val Ala Asp Pro Ala Met
lacZα
2500 2510 2520
TTCGCCATTCAGGCTGCGCAACTGTTGGGA
AAGCGGTAAGTCCGACGCGTTGACAACCCT
2520
Arg Trp Glu Pro Gln Ala Val Thr Pro Phe
lacZα
2530 2540 2550
AGGGCGATCGGTGCGGGCCTCTTCGCTATT
TCCCGCTAGCCACGCCCGGAGAAGCGATAA
2550
Pro Ser Arg His Pro Gly Arg Arg
Ter Ter
lacZα
2560 2570 2580
ACGCCAGCTGGCGAAAGGGGGATGTGCTGC
TGCGGTCGACCGCTTTCCCCCTACACGACG
2580
Ala Leu Gln Arg Phe Pro Ser Thr Ser Cys
lacZα
2590 2600 2610
AAGGCGATTAAGTTGGGTAACGCCAGGGTT
TTCCGCTAATTCAACCCATTGCGGTCCCAA
2610
Pro Ser Thr Pro Tyr Arg Trp Pro Lys
Ter
lacZα
2620 2630 2640
TTCCCAGTCACGACGTTGTAAAACGACGGC
AAGGGTCAGTGCTGCAACATTTTGCTGCCG
2640
Gly Leu Ser Thr Thr Phe Arg Arg Gly
Ter
lacZα
M13 fwd
pUC19 backbone reverse (2627 .. 2644)
2650 2660
CAGTATGATTGTCTTCGTGTTT
GTCATACTAACAGAAGCACAAA
2662
Thr
lacZα
M13 fwd
ATGA overhang
BbsI site
pUC19 backbone reverse (2627 .. 2644)
BbsI