Features
Primers
CDS
misc_feature
primer_bind
promoter
protein_bind
rep_origin
Circle
Line
Sequence
Zoom
Reset
500
1000
1500
2000
2500
3000
lacZα
GFP
ori
AmpR
M13 fwd
ATGA junction
MCS
TGGC junction
M13 rev
lac operator
lac promoter
AmpR promoter
pUC19 backbone forward (1117 .. 1140)
pUC19 backbone reverse (378 .. 395)
GFP forward (400 .. 424)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1155 .. 1182)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1232 .. 1251)
pUC19-GFP
3347 bp
500
1000
1500
2000
2500
3000
lacZα
M13 fwd
ATGA junction
GFP
TGGC junction
M13 rev
ori
AmpR
AmpR promoter
MCS
lac operator
lac promoter
pUC19 backbone forward (1117 .. 1140)
pUC19 backbone reverse (378 .. 395)
GFP forward (400 .. 424)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1155 .. 1182)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1232 .. 1251)
pUC19-GFP
3347 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
lacZα
M13 fwd
ATGA junction
GFP
TGGC junction
ori
AmpR
AmpR promoter
MCS
M13 rev
lac operator
lac promoter
pUC19 backbone forward (1117 .. 1140)
pUC19 backbone reverse (378 .. 395)
GFP forward (400 .. 424)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1155 .. 1182)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1232 .. 1251)
pUC19-GFP
3347 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
lacZα
M13 fwd
ATGA junction
GFP
TGGC junction
ori
AmpR
AmpR promoter
MCS
M13 rev
lac operator
lac promoter
pUC19 backbone forward (1117 .. 1140)
pUC19 backbone reverse (378 .. 395)
GFP forward (400 .. 424)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1155 .. 1182)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1232 .. 1251)
pUC19-GFP
3347 bp
50
100
150
200
250
300
350
400
450
500
550
600
650
700
750
800
850
900
950
1000
1050
1100
1150
1200
1250
1300
1350
1400
1450
1500
1550
1600
1650
1700
1750
1800
1850
1900
1950
2000
2050
2100
2150
2200
2250
2300
2350
2400
2450
2500
2550
2600
2650
2700
2750
2800
2850
2900
2950
3000
3050
3100
3150
3200
3250
3300
lacZα
M13 fwd
ATGA junction
GFP
MCS
TGGC junction
ori
AmpR
AmpR promoter
M13 rev
lac operator
lac promoter
pUC19 backbone forward (1117 .. 1140)
pUC19 backbone reverse (378 .. 395)
GFP forward (400 .. 424)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1155 .. 1182)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1232 .. 1251)
pUC19-GFP
3347 bp
Both strands
Copy
10 20 30 40 50 60
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
AGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGT
60
70 80 90 100 110 120
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
GTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
120
130 140 150 160 170 180
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
AACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACG
180
Ile Arg Cys Leu Leu Asn Tyr Gln Ser His
Ter
lacZα
190 200 210 220 230 240
ACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCC
TGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGG
240
Val Met His Pro Thr Phe Tyr Arg Val Ser Ala Tyr Pro Ser Phe Val Ala Asp Pro Ala
lacZα
250 260 270 280 290 300
ATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATA
300
Met Arg Trp Glu Pro Gln Ala Val Thr Pro Phe Pro Ser Arg His Pro Gly Arg Arg
Ter
lacZα
Sequencing forward (257 .. 272)
310 320 330 340 350 360
TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGT
ATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCCCA
360
Ala Leu Gln Arg Phe Pro Ser Thr Ser Cys Pro Ser Thr Pro Tyr Arg Trp Pro
Ter Ter
lacZα
Colony PCR forward (345 .. 365)
370 380 390 400 410 420
TTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTATGAGTAAAGGAGAAGAACTTTTCA
AAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCATACTCATTTCCTCTTCTTGAAAAGT
420
Lys Gly Leu Ser Thr Thr Phe Arg Arg Gly Thr
Ter
lacZα
M13 fwd
ATGA junction
Met Ser Lys Gly Glu Glu Leu Phe
GFP
Colony PCR forward (345 .. 365)
GFP forward (400 .. 424)
pUC19 backbone reverse (378 .. 395)
430 440 450 460 470 480
CTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTG
GACCTCAACAGGGTTAAGAACAACTTAATCTACCACTACAATTACCCGTGTTTAAAAGAC
480
Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
GFP
GFP forward (400 .. 424)
490 500 510 520 530 540
TCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCA
AGTCACCTCTCCCACTTCCACTACGTTGTATGCCTTTTGAATGGGAATTTAAATAAACGT
540
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys
GFP
Junction reverse (481 .. 504)
550 560 570 580 590 600
CTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTC
GATGACCTTTTGATGGACAAGGTACCGGTTGTGAACAGTGATGAAAGAGAATACCACAAG
600
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ser Tyr Gly Val
GFP
610 620 630 640 650 660
AATGCTTTTCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGC
TTACGAAAAGTTCTATGGGTCTAGTATACTTTGCCGTACTGAAAAAGTTCTCACGGTACG
660
Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met
GFP
670 680 690 700 710 720
CCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC
GGCTTCCAATACATGTCCTTTCTTGATATAAAAAGTTTCTACTGCCCTTGATGTTCTGTG
720
Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr
GFP
730 740 750 760 770 780
GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTG
CACGACTTCAGTTCAAACTTCCACTATGGGAACAATTATCTTAGCTCAATTTTCCATAAC
780
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile
GFP
790 800 810 820 830 840
ATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACA
TAAAATTTCTTCTACCTTTGTAAGAACCTGTGTTTAACCTTATGTTGATATTGAGTGTGT
840
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His
GFP
850 860 870 880 890 900
ATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGAC
TACATATGTAGTACCGTCTGTTTGTTTTCTTACCTTAGTTTCAATTGAAGTTTTAATCTG
900
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg
GFP
910 920 930 940 950 960
ACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTG
TGTTGTAACTTCTACCTTCGCAAGTTGATCGTCTGGTAATAGTTGTTTTATGAGGTTAAC
960
His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile
GFP
970 980 990 1000 1010 1020
GCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGA
CGCTACCGGGACAGGAAAATGGTCTGTTGGTAATGGACAGGTGTGTTAGACGGGAAAGCT
1020
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser
GFP
1030 1040 1050 1060 1070 1080
AAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGA
TTCTAGGGTTGCTTTTCTCTCTGGTGTACCAGGAAGAACTCAAACATTGTCGACGACCCT
1080
Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
GFP
1090 1100 1110 1120 1130 1140
TTACACATGGCATGGATGAACTATACAAATAGTGGCGTAATCATGGTCATAGCTGTTTCC
AATGTGTACCGTACCTACTTGATATGTTTATCACCGCATTAGTACCAGTATCGACAAAGG
1140
Pro Thr Ile Met Thr Met
lacZα
Ile Thr His Gly Met Asp Glu Leu Tyr Lys
Ter
GFP
MCS
TGGC junction
M13 rev
pUC19 backbone forward (1117 .. 1140)
GFP reverse (1087 .. 1112)
1150 1160 1170 1180 1190 1200
TGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTG
ACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCAC
1200
M13 rev
lac operator
lac promoter
Colony PCR reverse (1155 .. 1182)
1210 1220 1230 1240 1250 1260
TAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC
ATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGG
1260
lac promoter
Sequencing reverse (1232 .. 1251)
1270 1280 1290 1300 1310 1320
CGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG
GCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCC
1320
1330 1340 1350 1360 1370 1380
GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC
CTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAG
1380
1390 1400 1410 1420 1430 1440
GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC
CCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTG
1440
1450 1460 1470 1480 1490 1500
AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA
TCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTT
1500
1510 1520 1530 1540 1550 1560
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA
GGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGT
1560
ori
1570 1580 1590 1600 1610 1620
CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC
GTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCG
1620
ori
1630 1640 1650 1660 1670 1680
GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA
CAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTAT
1680
ori
1690 1700 1710 1720 1730 1740
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
GGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCAT
1740
ori
1750 1760 1770 1780 1790 1800
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA
AGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGT
1800
ori
1810 1820 1830 1840 1850 1860
GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA
CGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCT
1860
ori
1870 1880 1890 1900 1910 1920
CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG
GAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCC
1920
ori
1930 1940 1950 1960 1970 1980
TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGG
ACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACC
1980
ori
1990 2000 2010 2020 2030 2040
TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
ATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCC
2040
ori
2050 2060 2070 2080 2090 2100
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG
GTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTC
2100
ori
2110 2120 2130 2140 2150 2160
AAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA
TTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTT
2160
ori
2170 2180 2190 2200 2210 2220
CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT
GCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTA
2220
2230 2240 2250 2260 2270 2280
CCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTC
GGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAG
2280
2290 2300 2310 2320 2330 2340
TGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTC
ACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAG
2340
Trp His Lys Ile Leu Ser Ala Gly Ile Glu Ala Ile Gln Arg Asn Arg Glu
Ter
AmpR
2350 2360 2370 2380 2390 2400
ATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATC
TAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAG
2400
Asp Met Thr Ala Gln Ser Gly Thr Thr Tyr Ile Val Val Ile Arg Ser Pro Lys Gly Asp
AmpR
2410 2420 2430 2440 2450 2460
TGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGC
ACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCG
2460
Pro Gly Leu Ala Ala Ile Ile Gly Arg Ser Gly Arg Glu Gly Ala Gly Ser Lys Asp Ala
AmpR
2470 2480 2490 2500 2510 2520
AATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC
TTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAG
2520
Ile Phe Trp Gly Ala Pro Leu Ala Ser Arg Leu Leu Pro Gly Ala Val Lys Asp Ala Glu
AmpR
2530 2540 2550 2560 2570 2580
CATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT
GTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAA
2580
Met Trp Asp Ile Leu Gln Gln Arg Ser Ala Leu Thr Leu Leu Glu Gly Thr Leu Leu Lys
AmpR
2590 2600 2610 2620 2630 2640
GCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGC
CGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCG
2640
Arg Leu Thr Thr Ala Met Ala Val Pro Met Thr Thr Asp Arg Glu Asp Asn Pro Ile Ala
AmpR
2650 2660 2670 2680 2690 2700
TTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAA
AAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTT
2700
Glu Asn Leu Glu Pro Glu Trp Arg Asp Leu Arg Thr Val His Asp Gly Met Asn His Leu
AmpR
2710 2720 2730 2740 2750 2760
AAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTT
TTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAA
2760
Phe Ala Thr Leu Glu Lys Pro Gly Gly Ile Thr Thr Leu Leu Leu Asn Ala Ala Thr Asn
AmpR
2770 2780 2790 2800 2810 2820
ATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATG
TAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTAC
2820
Asp Ser Met Thr Ile Ala Ala Ser Cys Leu Glu Arg Val Thr Met Gly Asp Thr Leu His
AmpR
2830 2840 2850 2860 2870 2880
CTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACC
GAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGG
2880
Lys Glu Thr Val Pro Ser Tyr Glu Val Leu Asp Asn Gln Ser Tyr His Ile Arg Arg Gly
AmpR
2890 2900 2910 2920 2930 2940
GAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA
CTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTT
2940
Leu Gln Glu Gln Gly Ala Asp Ile Arg Ser Leu Val Ala Gly Cys Leu Leu Val Lys Phe
AmpR
2950 2960 2970 2980 2990 3000
AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTT
TCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAA
3000
Thr Ser Met Met Pro Phe Arg Glu Glu Pro Arg Phe Ser Glu Leu Ile Lys Gly Ser Asn
AmpR
3010 3020 3030 3040 3050 3060
GAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTT
CTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAA
3060
Leu Asp Leu Glu Ile Tyr Gly Val Arg Ala Gly Leu Gln Asp Glu Ala Asp Lys Val Lys
AmpR
3070 3080 3090 3100 3110 3120
CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAG
GTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTC
3120
Val Leu Thr Glu Pro His Ala Phe Val Pro Leu Cys Phe Ala Ala Phe Phe Pro Ile Leu
AmpR
3130 3140 3150 3160 3170 3180
GGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTA
CCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAAT
3180
Ala Val Arg Phe His Gln Ile Ser Met
AmpR
AmpR promoter
3190 3200 3210 3220 3230 3240
TCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAT
AGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTA
3240
AmpR promoter
3250 3260 3270 3280 3290 3300
AGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTAT
TCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATA
3300
AmpR promoter
3310 3320 3330 3340
CATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC
GTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAG
3347
10 20 30
TCGCGCGTTTCGGTGATGACGGTGAAAACC
AGCGCGCAAAGCCACTACTGCCACTTTTGG
30
40 50 60
TCTGACACATGCAGCTCCCGGAGACGGTCA
AGACTGTGTACGTCGAGGGCCTCTGCCAGT
60
70 80 90
CAGCTTGTCTGTAAGCGGATGCCGGGAGCA
GTCGAACAGACATTCGCCTACGGCCCTCGT
90
100 110 120
GACAAGCCCGTCAGGGCGCGTCAGCGGGTG
CTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
120
130 140 150
TTGGCGGGTGTCGGGGCTGGCTTAACTATG
AACCGCCCACAGCCCCGACCGAATTGATAC
150
Ile
lacZα
160 170 180
CGGCATCAGAGCAGATTGTACTGAGAGTGC
GCCGTAGTCTCGTCTAACATGACTCTCACG
180
Arg Cys Leu Leu Asn Tyr Gln Ser His
Ter
lacZα
190 200 210
ACCATATGCGGTGTGAAATACCGCACAGAT
TGGTATACGCCACACTTTATGGCGTGTCTA
210
Val Met His Pro Thr Phe Tyr Arg Val Ser
lacZα
220 230 240
GCGTAAGGAGAAAATACCGCATCAGGCGCC
CGCATTCCTCTTTTATGGCGTAGTCCGCGG
240
Ala Tyr Pro Ser Phe Val Ala Asp Pro Ala
lacZα
250 260 270
ATTCGCCATTCAGGCTGCGCAACTGTTGGG
TAAGCGGTAAGTCCGACGCGTTGACAACCC
270
Met Arg Trp Glu Pro Gln Ala Val Thr Pro
lacZα
Sequencing forward (257 .. 272)
280 290 300
AAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TTCCCGCTAGCCACGCCCGGAGAAGCGATA
300
Phe Pro Ser Arg His Pro Gly Arg Arg
Ter
lacZα
Sequencing forward (257 .. 272)
310 320 330
TACGCCAGCTGGCGAAAGGGGGATGTGCTG
ATGCGGTCGACCGCTTTCCCCCTACACGAC
330
Ala Leu Gln Arg Phe Pro Ser Thr Ser
Ter
lacZα
340 350 360
CAAGGCGATTAAGTTGGGTAACGCCAGGGT
GTTCCGCTAATTCAACCCATTGCGGTCCCA
360
Cys Pro Ser Thr Pro Tyr Arg Trp Pro
Ter
lacZα
Colony PCR forward (345 .. 365)
370 380 390
TTTCCCAGTCACGACGTTGTAAAACGACGG
AAAGGGTCAGTGCTGCAACATTTTGCTGCC
390
Lys Gly Leu Ser Thr Thr Phe Arg Arg
Ter
lacZα
M13 fwd
Colony PCR forward (345 .. 365)
pUC19 backbone reverse (378 .. 395)
400 410 420
CCAGTATGAGTAAAGGAGAAGAACTTTTCA
GGTCATACTCATTTCCTCTTCTTGAAAAGT
420
Gly Thr
lacZα
M13 fwd
ATGA junction
Met Ser Lys Gly Glu Glu Leu Phe
GFP
GFP forward (400 .. 424)
pUC19 backbone reverse (378 .. 395)
430 440 450
CTGGAGTTGTCCCAATTCTTGTTGAATTAG
GACCTCAACAGGGTTAAGAACAACTTAATC
450
Thr Gly Val Val Pro Ile Leu Val Glu Leu
GFP
GFP forward (400 .. 424)
460 470 480
ATGGTGATGTTAATGGGCACAAATTTTCTG
TACCACTACAATTACCCGTGTTTAAAAGAC
480
Asp Gly Asp Val Asn Gly His Lys Phe Ser
GFP
490 500 510
TCAGTGGAGAGGGTGAAGGTGATGCAACAT
AGTCACCTCTCCCACTTCCACTACGTTGTA
510
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr
GFP
Junction reverse (481 .. 504)
520 530 540
ACGGAAAACTTACCCTTAAATTTATTTGCA
TGCCTTTTGAATGGGAATTTAAATAAACGT
540
Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys
GFP
550 560 570
CTACTGGAAAACTACCTGTTCCATGGCCAA
GATGACCTTTTGATGGACAAGGTACCGGTT
570
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro
GFP
580 590 600
CACTTGTCACTACTTTCTCTTATGGTGTTC
GTGAACAGTGATGAAAGAGAATACCACAAG
600
Thr Leu Val Thr Thr Phe Ser Tyr Gly Val
GFP
610 620 630
AATGCTTTTCAAGATACCCAGATCATATGA
TTACGAAAAGTTCTATGGGTCTAGTATACT
630
Gln Cys Phe Ser Arg Tyr Pro Asp His Met
GFP
640 650 660
AACGGCATGACTTTTTCAAGAGTGCCATGC
TTGCCGTACTGAAAAAGTTCTCACGGTACG
660
Lys Arg His Asp Phe Phe Lys Ser Ala Met
GFP
670 680 690
CCGAAGGTTATGTACAGGAAAGAACTATAT
GGCTTCCAATACATGTCCTTTCTTGATATA
690
Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile
GFP
700 710 720
TTTTCAAAGATGACGGGAACTACAAGACAC
AAAAGTTTCTACTGCCCTTGATGTTCTGTG
720
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr
GFP
730 740 750
GTGCTGAAGTCAAGTTTGAAGGTGATACCC
CACGACTTCAGTTCAAACTTCCACTATGGG
750
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr
GFP
760 770 780
TTGTTAATAGAATCGAGTTAAAAGGTATTG
AACAATTATCTTAGCTCAATTTTCCATAAC
780
Leu Val Asn Arg Ile Glu Leu Lys Gly Ile
GFP
790 800 810
ATTTTAAAGAAGATGGAAACATTCTTGGAC
TAAAATTTCTTCTACCTTTGTAAGAACCTG
810
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
GFP
820 830 840
ACAAATTGGAATACAACTATAACTCACACA
TGTTTAACCTTATGTTGATATTGAGTGTGT
840
His Lys Leu Glu Tyr Asn Tyr Asn Ser His
GFP
850 860 870
ATGTATACATCATGGCAGACAAACAAAAGA
TACATATGTAGTACCGTCTGTTTGTTTTCT
870
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys
GFP
880 890 900
ATGGAATCAAAGTTAACTTCAAAATTAGAC
TACCTTAGTTTCAATTGAAGTTTTAATCTG
900
Asn Gly Ile Lys Val Asn Phe Lys Ile Arg
GFP
910 920 930
ACAACATTGAAGATGGAAGCGTTCAACTAG
TGTTGTAACTTCTACCTTCGCAAGTTGATC
930
His Asn Ile Glu Asp Gly Ser Val Gln Leu
GFP
940 950 960
CAGACCATTATCAACAAAATACTCCAATTG
GTCTGGTAATAGTTGTTTTATGAGGTTAAC
960
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile
GFP
970 980 990
GCGATGGCCCTGTCCTTTTACCAGACAACC
CGCTACCGGGACAGGAAAATGGTCTGTTGG
990
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
GFP
1000 1010 1020
ATTACCTGTCCACACAATCTGCCCTTTCGA
TAATGGACAGGTGTGTTAGACGGGAAAGCT
1020
His Tyr Leu Ser Thr Gln Ser Ala Leu Ser
GFP
1030 1040 1050
AAGATCCCAACGAAAAGAGAGACCACATGG
TTCTAGGGTTGCTTTTCTCTCTGGTGTACC
1050
Lys Asp Pro Asn Glu Lys Arg Asp His Met
GFP
1060 1070 1080
TCCTTCTTGAGTTTGTAACAGCTGCTGGGA
AGGAAGAACTCAAACATTGTCGACGACCCT
1080
Val Leu Leu Glu Phe Val Thr Ala Ala Gly
GFP
1090 1100 1110
TTACACATGGCATGGATGAACTATACAAAT
AATGTGTACCGTACCTACTTGATATGTTTA
1110
Ile Thr His Gly Met Asp Glu Leu Tyr Lys
GFP
GFP reverse (1087 .. 1112)
1120 1130 1140
AGTGGCGTAATCATGGTCATAGCTGTTTCC
TCACCGCATTAGTACCAGTATCGACAAAGG
1140
Pro Thr Ile Met Thr Met
lacZα
Ter
GFP
MCS
TGGC junction
M13 rev
pUC19 backbone forward (1117 .. 1140)
GFP reverse (1087 .. 1112)
1150 1160 1170
TGTGTGAAATTGTTATCCGCTCACAATTCC
ACACACTTTAACAATAGGCGAGTGTTAAGG
1170
M13 rev
lac operator
Colony PCR reverse (1155 .. 1182)
1180 1190 1200
ACACAACATACGAGCCGGAAGCATAAAGTG
TGTGTTGTATGCTCGGCCTTCGTATTTCAC
1200
lac promoter
Colony PCR reverse (1155 .. 1182)
1210 1220 1230
TAAAGCCTGGGGTGCCTAATGAGTGAGCTA
ATTTCGGACCCCACGGATTACTCACTCGAT
1230
lac promoter
1240 1250 1260
ACTCACATTAATTGCGTTGCGCTCACTGCC
TGAGTGTAATTAACGCAACGCGAGTGACGG
1260
Sequencing reverse (1232 .. 1251)
1270 1280 1290
CGCTTTCCAGTCGGGAAACCTGTCGTGCCA
GCGAAAGGTCAGCCCTTTGGACAGCACGGT
1290
1300 1310 1320
GCTGCATTAATGAATCGGCCAACGCGCGGG
CGACGTAATTACTTAGCCGGTTGCGCGCCC
1320
1330 1340 1350
GAGAGGCGGTTTGCGTATTGGGCGCTCTTC
CTCTCCGCCAAACGCATAACCCGCGAGAAG
1350
1360 1370 1380
CGCTTCCTCGCTCACTGACTCGCTGCGCTC
GCGAAGGAGCGAGTGACTGAGCGACGCGAG
1380
1390 1400 1410
GGTCGTTCGGCTGCGGCGAGCGGTATCAGC
CCAGCAAGCCGACGCCGCTCGCCATAGTCG
1410
1420 1430 1440
TCACTCAAAGGCGGTAATACGGTTATCCAC
AGTGAGTTTCCGCCATTATGCCAATAGGTG
1440
1450 1460 1470
AGAATCAGGGGATAACGCAGGAAAGAACAT
TCTTAGTCCCCTATTGCGTCCTTTCTTGTA
1470
1480 1490 1500
GTGAGCAAAAGGCCAGCAAAAGGCCAGGAA
CACTCGTTTTCCGGTCGTTTTCCGGTCCTT
1500
1510 1520 1530
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTT
GGCATTTTTCCGGCGCAACGACCGCAAAAA
1530
ori
1540 1550 1560
CCATAGGCTCCGCCCCCCTGACGAGCATCA
GGTATCCGAGGCGGGGGGACTGCTCGTAGT
1560
ori
1570 1580 1590
CAAAAATCGACGCTCAAGTCAGAGGTGGCG
GTTTTTAGCTGCGAGTTCAGTCTCCACCGC
1590
ori
1600 1610 1620
AAACCCGACAGGACTATAAAGATACCAGGC
TTTGGGCTGTCCTGATATTTCTATGGTCCG
1620
ori
1630 1640 1650
GTTTCCCCCTGGAAGCTCCCTCGTGCGCTC
CAAAGGGGGACCTTCGAGGGAGCACGCGAG
1650
ori
1660 1670 1680
TCCTGTTCCGACCCTGCCGCTTACCGGATA
AGGACAAGGCTGGGACGGCGAATGGCCTAT
1680
ori
1690 1700 1710
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGT
GGACAGGCGGAAAGAGGGAAGCCCTTCGCA
1710
ori
1720 1730 1740
GGCGCTTTCTCATAGCTCACGCTGTAGGTA
CCGCGAAAGAGTATCGAGTGCGACATCCAT
1740
ori
1750 1760 1770
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAA
AGAGTCAAGCCACATCCAGCAAGCGAGGTT
1770
ori
1780 1790 1800
GCTGGGCTGTGTGCACGAACCCCCCGTTCA
CGACCCGACACACGTGCTTGGGGGGCAAGT
1800
ori
1810 1820 1830
GCCCGACCGCTGCGCCTTATCCGGTAACTA
CGGGCTGGCGACGCGGAATAGGCCATTGAT
1830
ori
1840 1850 1860
TCGTCTTGAGTCCAACCCGGTAAGACACGA
AGCAGAACTCAGGTTGGGCCATTCTGTGCT
1860
ori
1870 1880 1890
CTTATCGCCACTGGCAGCAGCCACTGGTAA
GAATAGCGGTGACCGTCGTCGGTGACCATT
1890
ori
1900 1910 1920
CAGGATTAGCAGAGCGAGGTATGTAGGCGG
GTCCTAATCGTCTCGCTCCATACATCCGCC
1920
ori
1930 1940 1950
TGCTACAGAGTTCTTGAAGTGGTGGCCTAA
ACGATGTCTCAAGAACTTCACCACCGGATT
1950
ori
1960 1970 1980
CTACGGCTACACTAGAAGAACAGTATTTGG
GATGCCGATGTGATCTTCTTGTCATAAACC
1980
ori
1990 2000 2010
TATCTGCGCTCTGCTGAAGCCAGTTACCTT
ATAGACGCGAGACGACTTCGGTCAATGGAA
2010
ori
2020 2030 2040
CGGAAAAAGAGTTGGTAGCTCTTGATCCGG
GCCTTTTTCTCAACCATCGAGAACTAGGCC
2040
ori
2050 2060 2070
CAAACAAACCACCGCTGGTAGCGGTGGTTT
GTTTGTTTGGTGGCGACCATCGCCACCAAA
2070
ori
2080 2090 2100
TTTTGTTTGCAAGCAGCAGATTACGCGCAG
AAAACAAACGTTCGTCGTCTAATGCGCGTC
2100
ori
2110 2120 2130
AAAAAAAGGATCTCAAGAAGATCCTTTGAT
TTTTTTTCCTAGAGTTCTTCTAGGAAACTA
2130
ori
2140 2150 2160
CTTTTCTACGGGGTCTGACGCTCAGTGGAA
GAAAAGATGCCCCAGACTGCGAGTCACCTT
2160
2170 2180 2190
CGAAAACTCACGTTAAGGGATTTTGGTCAT
GCTTTTGAGTGCAATTCCCTAAAACCAGTA
2190
2200 2210 2220
GAGATTATCAAAAAGGATCTTCACCTAGAT
CTCTAATAGTTTTTCCTAGAAGTGGATCTA
2220
2230 2240 2250
CCTTTTAAATTAAAAATGAAGTTTTAAATC
GGAAAATTTAATTTTTACTTCAAAATTTAG
2250
2260 2270 2280
AATCTAAAGTATATATGAGTAAACTTGGTC
TTAGATTTCATATATACTCATTTGAACCAG
2280
2290 2300 2310
TGACAGTTACCAATGCTTAATCAGTGAGGC
ACTGTCAATGGTTACGAATTAGTCACTCCG
2310
Trp His Lys Ile Leu Ser Ala
Ter
AmpR
2320 2330 2340
ACCTATCTCAGCGATCTGTCTATTTCGTTC
TGGATAGAGTCGCTAGACAGATAAAGCAAG
2340
Gly Ile Glu Ala Ile Gln Arg Asn Arg Glu
AmpR
2350 2360 2370
ATCCATAGTTGCCTGACTCCCCGTCGTGTA
TAGGTATCAACGGACTGAGGGGCAGCACAT
2370
Asp Met Thr Ala Gln Ser Gly Thr Thr Tyr
AmpR
2380 2390 2400
GATAACTACGATACGGGAGGGCTTACCATC
CTATTGATGCTATGCCCTCCCGAATGGTAG
2400
Ile Val Val Ile Arg Ser Pro Lys Gly Asp
AmpR
2410 2420 2430
TGGCCCCAGTGCTGCAATGATACCGCGAGA
ACCGGGGTCACGACGTTACTATGGCGCTCT
2430
Pro Gly Leu Ala Ala Ile Ile Gly Arg Ser
AmpR
2440 2450 2460
CCCACGCTCACCGGCTCCAGATTTATCAGC
GGGTGCGAGTGGCCGAGGTCTAAATAGTCG
2460
Gly Arg Glu Gly Ala Gly Ser Lys Asp Ala
AmpR
2470 2480 2490
AATAAACCAGCCAGCCGGAAGGGCCGAGCG
TTATTTGGTCGGTCGGCCTTCCCGGCTCGC
2490
Ile Phe Trp Gly Ala Pro Leu Ala Ser Arg
AmpR
2500 2510 2520
CAGAAGTGGTCCTGCAACTTTATCCGCCTC
GTCTTCACCAGGACGTTGAAATAGGCGGAG
2520
Leu Leu Pro Gly Ala Val Lys Asp Ala Glu
AmpR
2530 2540 2550
CATCCAGTCTATTAATTGTTGCCGGGAAGC
GTAGGTCAGATAATTAACAACGGCCCTTCG
2550
Met Trp Asp Ile Leu Gln Gln Arg Ser Ala
AmpR
2560 2570 2580
TAGAGTAAGTAGTTCGCCAGTTAATAGTTT
ATCTCATTCATCAAGCGGTCAATTATCAAA
2580
Leu Thr Leu Leu Glu Gly Thr Leu Leu Lys
AmpR
2590 2600 2610
GCGCAACGTTGTTGCCATTGCTACAGGCAT
CGCGTTGCAACAACGGTAACGATGTCCGTA
2610
Arg Leu Thr Thr Ala Met Ala Val Pro Met
AmpR
2620 2630 2640
CGTGGTGTCACGCTCGTCGTTTGGTATGGC
GCACCACAGTGCGAGCAGCAAACCATACCG
2640
Thr Thr Asp Arg Glu Asp Asn Pro Ile Ala
AmpR
2650 2660 2670
TTCATTCAGCTCCGGTTCCCAACGATCAAG
AAGTAAGTCGAGGCCAAGGGTTGCTAGTTC
2670
Glu Asn Leu Glu Pro Glu Trp Arg Asp Leu
AmpR
2680 2690 2700
GCGAGTTACATGATCCCCCATGTTGTGCAA
CGCTCAATGTACTAGGGGGTACAACACGTT
2700
Arg Thr Val His Asp Gly Met Asn His Leu
AmpR
2710 2720 2730
AAAAGCGGTTAGCTCCTTCGGTCCTCCGAT
TTTTCGCCAATCGAGGAAGCCAGGAGGCTA
2730
Phe Ala Thr Leu Glu Lys Pro Gly Gly Ile
AmpR
2740 2750 2760
CGTTGTCAGAAGTAAGTTGGCCGCAGTGTT
GCAACAGTCTTCATTCAACCGGCGTCACAA
2760
Thr Thr Leu Leu Leu Asn Ala Ala Thr Asn
AmpR
2770 2780 2790
ATCACTCATGGTTATGGCAGCACTGCATAA
TAGTGAGTACCAATACCGTCGTGACGTATT
2790
Asp Ser Met Thr Ile Ala Ala Ser Cys Leu
AmpR
2800 2810 2820
TTCTCTTACTGTCATGCCATCCGTAAGATG
AAGAGAATGACAGTACGGTAGGCATTCTAC
2820
Glu Arg Val Thr Met Gly Asp Thr Leu His
AmpR
2830 2840 2850
CTTTTCTGTGACTGGTGAGTACTCAACCAA
GAAAAGACACTGACCACTCATGAGTTGGTT
2850
Lys Glu Thr Val Pro Ser Tyr Glu Val Leu
AmpR
2860 2870 2880
GTCATTCTGAGAATAGTGTATGCGGCGACC
CAGTAAGACTCTTATCACATACGCCGCTGG
2880
Asp Asn Gln Ser Tyr His Ile Arg Arg Gly
AmpR
2890 2900 2910
GAGTTGCTCTTGCCCGGCGTCAATACGGGA
CTCAACGAGAACGGGCCGCAGTTATGCCCT
2910
Leu Gln Glu Gln Gly Ala Asp Ile Arg Ser
AmpR
2920 2930 2940
TAATACCGCGCCACATAGCAGAACTTTAAA
ATTATGGCGCGGTGTATCGTCTTGAAATTT
2940
Leu Val Ala Gly Cys Leu Leu Val Lys Phe
AmpR
2950 2960 2970
AGTGCTCATCATTGGAAAACGTTCTTCGGG
TCACGAGTAGTAACCTTTTGCAAGAAGCCC
2970
Thr Ser Met Met Pro Phe Arg Glu Glu Pro
AmpR
2980 2990 3000
GCGAAAACTCTCAAGGATCTTACCGCTGTT
CGCTTTTGAGAGTTCCTAGAATGGCGACAA
3000
Arg Phe Ser Glu Leu Ile Lys Gly Ser Asn
AmpR
3010 3020 3030
GAGATCCAGTTCGATGTAACCCACTCGTGC
CTCTAGGTCAAGCTACATTGGGTGAGCACG
3030
Leu Asp Leu Glu Ile Tyr Gly Val Arg Ala
AmpR
3040 3050 3060
ACCCAACTGATCTTCAGCATCTTTTACTTT
TGGGTTGACTAGAAGTCGTAGAAAATGAAA
3060
Gly Leu Gln Asp Glu Ala Asp Lys Val Lys
AmpR
3070 3080 3090
CACCAGCGTTTCTGGGTGAGCAAAAACAGG
GTGGTCGCAAAGACCCACTCGTTTTTGTCC
3090
Val Leu Thr Glu Pro His Ala Phe Val Pro
AmpR
3100 3110 3120
AAGGCAAAATGCCGCAAAAAAGGGAATAAG
TTCCGTTTTACGGCGTTTTTTCCCTTATTC
3120
Leu Cys Phe Ala Ala Phe Phe Pro Ile Leu
AmpR
3130 3140 3150
GGCGACACGGAAATGTTGAATACTCATACT
CCGCTGTGCCTTTACAACTTATGAGTATGA
3150
Ala Val Arg Phe His Gln Ile Ser Met
AmpR
AmpR promoter
3160 3170 3180
CTTCCTTTTTCAATATTATTGAAGCATTTA
GAAGGAAAAAGTTATAATAACTTCGTAAAT
3180
AmpR promoter
3190 3200 3210
TCAGGGTTATTGTCTCATGAGCGGATACAT
AGTCCCAATAACAGAGTACTCGCCTATGTA
3210
AmpR promoter
3220 3230 3240
ATTTGAATGTATTTAGAAAAATAAACAAAT
TAAACTTACATAAATCTTTTTATTTGTTTA
3240
AmpR promoter
3250 3260 3270
AGGGGTTCCGCGCACATTTCCCCGAAAAGT
TCCCCAAGGCGCGTGTAAAGGGGCTTTTCA
3270
AmpR promoter
3280 3290 3300
GCCACCTGACGTCTAAGAAACCATTATTAT
CGGTGGACTGCAGATTCTTTGGTAATAATA
3300
3310 3320 3330
CATGACATTAACCTATAAAAATAGGCGTAT
GTACTGTAATTGGATATTTTTATCCGCATA
3330
3340
CACGAGGCCCTTTCGTC
GTGCTCCGGGAAAGCAG
3347