name	sequence	length	tm_c
pUC19 backbone forward	AAACACGAAGACAATGGCGTAATCATGGTCATAGCTGTTTCC	42	63.0
pUC19 backbone reverse	AAACACGAAGACAATCATACTGGCCGTCGTTTTACA	36	64.1
GFP forward	AAACACGAAGACAAATGAGTAAAGGAGAAGAACTTTTCACTGG	43	63.0
GFP reverse	AAACACGAAGACAAGCCACTATTTGTATAGTTCATCCATGCCATG	45	63.1
Colony PCR forward	CGATTAAGTTGGGTAACGCCAGGGTTTT	28	62.4
Colony PCR reverse	GTTAGCTCACTCATTAGGCACCCCA	25	62.2
Junction reverse	CACCCTCTCCACTGACAGAAAATTTGTGC	29	62.9
Sequencing forward	AACTGTTGGGAAGGGC	16	62.0
Sequencing reverse	ACGCAATTAATGTGAGTTAGCT	22	61.7