Features
Primers
CDS
misc_feature
primer_bind
promoter
protein_bind
rep_origin
Circle
Line
Sequence
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500
1000
1500
2000
2500
3000
lacZα
GFP
ori
AmpR
M13 fwd
pUC19 backbone-GFP overlap
GFP-pUC19 backbone overlap
M13 rev
lac operator
lac promoter
AmpR promoter
pUC19 backbone forward (1113 .. 1132)
pUC19 backbone reverse (378 .. 395)
GFP forward (396 .. 421)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1154 .. 1181)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1231 .. 1250)
pUC19-GFP
3346 bp
500
1000
1500
2000
2500
3000
lacZα
M13 fwd
pUC19 backbone-GFP overlap
GFP
M13 rev
ori
AmpR
AmpR promoter
GFP-pUC19 backbone overlap
lac operator
lac promoter
pUC19 backbone forward (1113 .. 1132)
pUC19 backbone reverse (378 .. 395)
GFP forward (396 .. 421)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1154 .. 1181)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1231 .. 1250)
pUC19-GFP
3346 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
lacZα
M13 fwd
pUC19 backbone-GFP overlap
GFP
GFP-pUC19 backbone overlap
M13 rev
ori
AmpR
AmpR promoter
lac operator
lac promoter
pUC19 backbone forward (1113 .. 1132)
pUC19 backbone reverse (378 .. 395)
GFP forward (396 .. 421)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1154 .. 1181)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1231 .. 1250)
pUC19-GFP
3346 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
lacZα
M13 fwd
pUC19 backbone-GFP overlap
GFP
GFP-pUC19 backbone overlap
M13 rev
ori
AmpR
AmpR promoter
lac operator
lac promoter
pUC19 backbone forward (1113 .. 1132)
pUC19 backbone reverse (378 .. 395)
GFP forward (396 .. 421)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1154 .. 1181)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1231 .. 1250)
pUC19-GFP
3346 bp
50
100
150
200
250
300
350
400
450
500
550
600
650
700
750
800
850
900
950
1000
1050
1100
1150
1200
1250
1300
1350
1400
1450
1500
1550
1600
1650
1700
1750
1800
1850
1900
1950
2000
2050
2100
2150
2200
2250
2300
2350
2400
2450
2500
2550
2600
2650
2700
2750
2800
2850
2900
2950
3000
3050
3100
3150
3200
3250
3300
lacZα
M13 fwd
pUC19 backbone-GFP overlap
GFP
GFP-pUC19 backbone overlap
M13 rev
ori
AmpR
AmpR promoter
lac operator
lac promoter
pUC19 backbone forward (1113 .. 1132)
pUC19 backbone reverse (378 .. 395)
GFP forward (396 .. 421)
GFP reverse (1087 .. 1112)
Colony PCR forward (345 .. 365)
Colony PCR reverse (1154 .. 1181)
Junction reverse (481 .. 504)
Sequencing forward (257 .. 272)
Sequencing reverse (1231 .. 1250)
pUC19-GFP
3346 bp
Both strands
Copy
10 20 30 40 50 60
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
AGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGT
60
70 80 90 100 110 120
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
GTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
120
130 140 150 160 170 180
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
AACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACG
180
Ala Ala Asp Ser Cys Ile Thr Ser Leu Thr Cys
Ter
lacZα
190 200 210 220 230 240
ACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCC
TGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGG
240
Trp Ile Arg His Ser Ile Gly Cys Leu His Thr Leu Leu Phe Tyr Arg Met Leu Arg Trp
lacZα
250 260 270 280 290 300
ATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATA
300
Glu Gly Asn Leu Ser Arg Leu Gln Gln Ser Pro Arg Asp Thr Arg Ala Glu Glu Ser Asn
lacZα
Sequencing forward (257 .. 272)
310 320 330 340 350 360
TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGT
ATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCCCA
360
Arg Trp Ser Ala Phe Pro Pro His Ala Ala Leu Arg Asn Leu Gln Thr Val Gly Pro Asn
lacZα
Colony PCR forward (345 .. 365)
370 380 390 400 410 420
TTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTATGAGTAAAGGAGAAGAACTTTTCA
AAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCATACTCATTTCCTCTTCTTGAAAAGT
420
Glu Trp Asp Arg Arg Gln Leu Val Val Ala Leu
lacZα
M13 fwd
pUC19 backbone-GFP overlap
Met Ser Lys Gly Glu Glu Leu Phe
GFP
Colony PCR forward (345 .. 365)
GFP forward (396 .. 421)
pUC19 backbone reverse (378 .. 395)
430 440 450 460 470 480
CTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTG
GACCTCAACAGGGTTAAGAACAACTTAATCTACCACTACAATTACCCGTGTTTAAAAGAC
480
Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser
GFP
GFP forward (396 .. 421)
490 500 510 520 530 540
TCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCA
AGTCACCTCTCCCACTTCCACTACGTTGTATGCCTTTTGAATGGGAATTTAAATAAACGT
540
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys
GFP
Junction reverse (481 .. 504)
550 560 570 580 590 600
CTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTC
GATGACCTTTTGATGGACAAGGTACCGGTTGTGAACAGTGATGAAAGAGAATACCACAAG
600
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ser Tyr Gly Val
GFP
610 620 630 640 650 660
AATGCTTTTCAAGATACCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGC
TTACGAAAAGTTCTATGGGTCTAGTATACTTTGCCGTACTGAAAAAGTTCTCACGGTACG
660
Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met
GFP
670 680 690 700 710 720
CCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACAC
GGCTTCCAATACATGTCCTTTCTTGATATAAAAAGTTTCTACTGCCCTTGATGTTCTGTG
720
Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr
GFP
730 740 750 760 770 780
GTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTG
CACGACTTCAGTTCAAACTTCCACTATGGGAACAATTATCTTAGCTCAATTTTCCATAAC
780
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile
GFP
790 800 810 820 830 840
ATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACA
TAAAATTTCTTCTACCTTTGTAAGAACCTGTGTTTAACCTTATGTTGATATTGAGTGTGT
840
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His
GFP
850 860 870 880 890 900
ATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGAC
TACATATGTAGTACCGTCTGTTTGTTTTCTTACCTTAGTTTCAATTGAAGTTTTAATCTG
900
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg
GFP
910 920 930 940 950 960
ACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTG
TGTTGTAACTTCTACCTTCGCAAGTTGATCGTCTGGTAATAGTTGTTTTATGAGGTTAAC
960
His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile
GFP
970 980 990 1000 1010 1020
GCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGA
CGCTACCGGGACAGGAAAATGGTCTGTTGGTAATGGACAGGTGTGTTAGACGGGAAAGCT
1020
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser
GFP
1030 1040 1050 1060 1070 1080
AAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGA
TTCTAGGGTTGCTTTTCTCTCTGGTGTACCAGGAAGAACTCAAACATTGTCGACGACCCT
1080
Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly
GFP
1090 1100 1110 1120 1130 1140
TTACACATGGCATGGATGAACTATACAAATAGGGCGTAATCATGGTCATAGCTGTTTCCT
AATGTGTACCGTACCTACTTGATATGTTTATCCCGCATTAGTACCAGTATCGACAAAGGA
1140
Pro Thr Ile Met Thr Met
lacZα
Ile Thr His Gly Met Asp Glu Leu Tyr Lys
Ter
GFP
GFP-pUC19 backbone overlap
M13 rev
pUC19 backbone forward (1113 .. 1132)
GFP reverse (1087 .. 1112)
1150 1160 1170 1180 1190 1200
GTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGT
CACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACA
1200
M13 rev
lac operator
lac promoter
Colony PCR reverse (1154 .. 1181)
1210 1220 1230 1240 1250 1260
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCC
TTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGG
1260
lac promoter
Sequencing reverse (1231 .. 1250)
1270 1280 1290 1300 1310 1320
GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGG
CGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCC
1320
1330 1340 1350 1360 1370 1380
AGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG
TCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGC
1380
1390 1400 1410 1420 1430 1440
GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA
CAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGT
1440
1450 1460 1470 1480 1490 1500
GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC
CTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTG
1500
1510 1520 1530 1540 1550 1560
CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCAC
GCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTG
1560
ori
1570 1580 1590 1600 1610 1620
AAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
TTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGC
1620
ori
1630 1640 1650 1660 1670 1680
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC
AAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATG
1680
ori
1690 1700 1710 1720 1730 1740
CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTAT
GACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATA
1740
ori
1750 1760 1770 1780 1790 1800
CTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAG
GAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTC
1800
ori
1810 1820 1830 1840 1850 1860
CCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
GGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTG
1860
ori
1870 1880 1890 1900 1910 1920
TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT
AATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCA
1920
ori
1930 1940 1950 1960 1970 1980
GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGT
CGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCA
1980
ori
1990 2000 2010 2020 2030 2040
ATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGC
TAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCG
2040
ori
2050 2060 2070 2080 2090 2100
AAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA
TTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCT
2100
ori
2110 2120 2130 2140 2150 2160
AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAAC
TTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTG
2160
ori
2170 2180 2190 2200 2210 2220
GAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC
CTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAG
2220
2230 2240 2250 2260 2270 2280
CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT
GAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGA
2280
2290 2300 2310 2320 2330 2340
GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCA
CTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGT
2340
Trp His Lys Ile Leu Ser Ala Gly Ile Glu Ala Ile Gln Arg Asn Arg Glu
Ter
AmpR
2350 2360 2370 2380 2390 2400
TCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCT
AGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGA
2400
Asp Met Thr Ala Gln Ser Gly Thr Thr Tyr Ile Val Val Ile Arg Ser Pro Lys Gly Asp
AmpR
2410 2420 2430 2440 2450 2460
GGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCA
CCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGT
2460
Pro Gly Leu Ala Ala Ile Ile Gly Arg Ser Gly Arg Glu Gly Ala Gly Ser Lys Asp Ala
AmpR
2470 2480 2490 2500 2510 2520
ATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC
TATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGG
2520
Ile Phe Trp Gly Ala Pro Leu Ala Ser Arg Leu Leu Pro Gly Ala Val Lys Asp Ala Glu
AmpR
2530 2540 2550 2560 2570 2580
ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG
TAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAAC
2580
Met Trp Asp Ile Leu Gln Gln Arg Ser Ala Leu Thr Leu Leu Glu Gly Thr Leu Leu Lys
AmpR
2590 2600 2610 2620 2630 2640
CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCT
GCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGA
2640
Arg Leu Thr Thr Ala Met Ala Val Pro Met Thr Thr Asp Arg Glu Asp Asn Pro Ile Ala
AmpR
2650 2660 2670 2680 2690 2700
TCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA
AGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTT
2700
Glu Asn Leu Glu Pro Glu Trp Arg Asp Leu Arg Thr Val His Asp Gly Met Asn His Leu
AmpR
2710 2720 2730 2740 2750 2760
AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTA
TTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAAT
2760
Phe Ala Thr Leu Glu Lys Pro Gly Gly Ile Thr Thr Leu Leu Leu Asn Ala Ala Thr Asn
AmpR
2770 2780 2790 2800 2810 2820
TCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGC
AGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACG
2820
Asp Ser Met Thr Ile Ala Ala Ser Cys Leu Glu Arg Val Thr Met Gly Asp Thr Leu His
AmpR
2830 2840 2850 2860 2870 2880
TTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCG
AAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGC
2880
Lys Glu Thr Val Pro Ser Tyr Glu Val Leu Asp Asn Gln Ser Tyr His Ile Arg Arg Gly
AmpR
2890 2900 2910 2920 2930 2940
AGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAA
TCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTT
2940
Leu Gln Glu Gln Gly Ala Asp Ile Arg Ser Leu Val Ala Gly Cys Leu Leu Val Lys Phe
AmpR
2950 2960 2970 2980 2990 3000
GTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG
CACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAAC
3000
Thr Ser Met Met Pro Phe Arg Glu Glu Pro Arg Phe Ser Glu Leu Ile Lys Gly Ser Asn
AmpR
3010 3020 3030 3040 3050 3060
AGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTC
TCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAG
3060
Leu Asp Leu Glu Ile Tyr Gly Val Arg Ala Gly Leu Gln Asp Glu Ala Asp Lys Val Lys
AmpR
3070 3080 3090 3100 3110 3120
ACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG
TGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCC
3120
Val Leu Thr Glu Pro His Ala Phe Val Pro Leu Cys Phe Ala Ala Phe Phe Pro Ile Leu
AmpR
3130 3140 3150 3160 3170 3180
GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT
CGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATA
3180
Ala Val Arg Phe His Gln Ile Ser Met
AmpR
AmpR promoter
3190 3200 3210 3220 3230 3240
CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATA
GTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTAT
3240
AmpR promoter
3250 3260 3270 3280 3290 3300
GGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATC
CCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAG
3300
AmpR promoter
3310 3320 3330 3340
ATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC
TACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAG
3346
10 20 30
TCGCGCGTTTCGGTGATGACGGTGAAAACC
AGCGCGCAAAGCCACTACTGCCACTTTTGG
30
40 50 60
TCTGACACATGCAGCTCCCGGAGACGGTCA
AGACTGTGTACGTCGAGGGCCTCTGCCAGT
60
70 80 90
CAGCTTGTCTGTAAGCGGATGCCGGGAGCA
GTCGAACAGACATTCGCCTACGGCCCTCGT
90
100 110 120
GACAAGCCCGTCAGGGCGCGTCAGCGGGTG
CTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
120
130 140 150
TTGGCGGGTGTCGGGGCTGGCTTAACTATG
AACCGCCCACAGCCCCGACCGAATTGATAC
150
Ala
Ter
lacZα
160 170 180
CGGCATCAGAGCAGATTGTACTGAGAGTGC
GCCGTAGTCTCGTCTAACATGACTCTCACG
180
Ala Asp Ser Cys Ile Thr Ser Leu Thr Cys
lacZα
190 200 210
ACCATATGCGGTGTGAAATACCGCACAGAT
TGGTATACGCCACACTTTATGGCGTGTCTA
210
Trp Ile Arg His Ser Ile Gly Cys Leu His
lacZα
220 230 240
GCGTAAGGAGAAAATACCGCATCAGGCGCC
CGCATTCCTCTTTTATGGCGTAGTCCGCGG
240
Thr Leu Leu Phe Tyr Arg Met Leu Arg Trp
lacZα
250 260 270
ATTCGCCATTCAGGCTGCGCAACTGTTGGG
TAAGCGGTAAGTCCGACGCGTTGACAACCC
270
Glu Gly Asn Leu Ser Arg Leu Gln Gln Ser
lacZα
Sequencing forward (257 .. 272)
280 290 300
AAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TTCCCGCTAGCCACGCCCGGAGAAGCGATA
300
Pro Arg Asp Thr Arg Ala Glu Glu Ser Asn
lacZα
Sequencing forward (257 .. 272)
310 320 330
TACGCCAGCTGGCGAAAGGGGGATGTGCTG
ATGCGGTCGACCGCTTTCCCCCTACACGAC
330
Arg Trp Ser Ala Phe Pro Pro His Ala Ala
lacZα
340 350 360
CAAGGCGATTAAGTTGGGTAACGCCAGGGT
GTTCCGCTAATTCAACCCATTGCGGTCCCA
360
Leu Arg Asn Leu Gln Thr Val Gly Pro Asn
lacZα
Colony PCR forward (345 .. 365)
370 380 390
TTTCCCAGTCACGACGTTGTAAAACGACGG
AAAGGGTCAGTGCTGCAACATTTTGCTGCC
390
Glu Trp Asp Arg Arg Gln Leu Val Val Ala
lacZα
M13 fwd
pUC19 backbone-GFP overlap
Colony PCR forward (345 .. 365)
pUC19 backbone reverse (378 .. 395)
400 410 420
CCAGTATGAGTAAAGGAGAAGAACTTTTCA
GGTCATACTCATTTCCTCTTCTTGAAAAGT
420
Leu
lacZα
M13 fwd
pUC19 backbone-GFP overlap
Met Ser Lys Gly Glu Glu Leu Phe
GFP
GFP forward (396 .. 421)
pUC19 backbone reverse (378 .. 395)
430 440 450
CTGGAGTTGTCCCAATTCTTGTTGAATTAG
GACCTCAACAGGGTTAAGAACAACTTAATC
450
Thr Gly Val Val Pro Ile Leu Val Glu Leu
GFP
GFP forward (396 .. 421)
460 470 480
ATGGTGATGTTAATGGGCACAAATTTTCTG
TACCACTACAATTACCCGTGTTTAAAAGAC
480
Asp Gly Asp Val Asn Gly His Lys Phe Ser
GFP
490 500 510
TCAGTGGAGAGGGTGAAGGTGATGCAACAT
AGTCACCTCTCCCACTTCCACTACGTTGTA
510
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr
GFP
Junction reverse (481 .. 504)
520 530 540
ACGGAAAACTTACCCTTAAATTTATTTGCA
TGCCTTTTGAATGGGAATTTAAATAAACGT
540
Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys
GFP
550 560 570
CTACTGGAAAACTACCTGTTCCATGGCCAA
GATGACCTTTTGATGGACAAGGTACCGGTT
570
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro
GFP
580 590 600
CACTTGTCACTACTTTCTCTTATGGTGTTC
GTGAACAGTGATGAAAGAGAATACCACAAG
600
Thr Leu Val Thr Thr Phe Ser Tyr Gly Val
GFP
610 620 630
AATGCTTTTCAAGATACCCAGATCATATGA
TTACGAAAAGTTCTATGGGTCTAGTATACT
630
Gln Cys Phe Ser Arg Tyr Pro Asp His Met
GFP
640 650 660
AACGGCATGACTTTTTCAAGAGTGCCATGC
TTGCCGTACTGAAAAAGTTCTCACGGTACG
660
Lys Arg His Asp Phe Phe Lys Ser Ala Met
GFP
670 680 690
CCGAAGGTTATGTACAGGAAAGAACTATAT
GGCTTCCAATACATGTCCTTTCTTGATATA
690
Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile
GFP
700 710 720
TTTTCAAAGATGACGGGAACTACAAGACAC
AAAAGTTTCTACTGCCCTTGATGTTCTGTG
720
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr
GFP
730 740 750
GTGCTGAAGTCAAGTTTGAAGGTGATACCC
CACGACTTCAGTTCAAACTTCCACTATGGG
750
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr
GFP
760 770 780
TTGTTAATAGAATCGAGTTAAAAGGTATTG
AACAATTATCTTAGCTCAATTTTCCATAAC
780
Leu Val Asn Arg Ile Glu Leu Lys Gly Ile
GFP
790 800 810
ATTTTAAAGAAGATGGAAACATTCTTGGAC
TAAAATTTCTTCTACCTTTGTAAGAACCTG
810
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
GFP
820 830 840
ACAAATTGGAATACAACTATAACTCACACA
TGTTTAACCTTATGTTGATATTGAGTGTGT
840
His Lys Leu Glu Tyr Asn Tyr Asn Ser His
GFP
850 860 870
ATGTATACATCATGGCAGACAAACAAAAGA
TACATATGTAGTACCGTCTGTTTGTTTTCT
870
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys
GFP
880 890 900
ATGGAATCAAAGTTAACTTCAAAATTAGAC
TACCTTAGTTTCAATTGAAGTTTTAATCTG
900
Asn Gly Ile Lys Val Asn Phe Lys Ile Arg
GFP
910 920 930
ACAACATTGAAGATGGAAGCGTTCAACTAG
TGTTGTAACTTCTACCTTCGCAAGTTGATC
930
His Asn Ile Glu Asp Gly Ser Val Gln Leu
GFP
940 950 960
CAGACCATTATCAACAAAATACTCCAATTG
GTCTGGTAATAGTTGTTTTATGAGGTTAAC
960
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile
GFP
970 980 990
GCGATGGCCCTGTCCTTTTACCAGACAACC
CGCTACCGGGACAGGAAAATGGTCTGTTGG
990
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn
GFP
1000 1010 1020
ATTACCTGTCCACACAATCTGCCCTTTCGA
TAATGGACAGGTGTGTTAGACGGGAAAGCT
1020
His Tyr Leu Ser Thr Gln Ser Ala Leu Ser
GFP
1030 1040 1050
AAGATCCCAACGAAAAGAGAGACCACATGG
TTCTAGGGTTGCTTTTCTCTCTGGTGTACC
1050
Lys Asp Pro Asn Glu Lys Arg Asp His Met
GFP
1060 1070 1080
TCCTTCTTGAGTTTGTAACAGCTGCTGGGA
AGGAAGAACTCAAACATTGTCGACGACCCT
1080
Val Leu Leu Glu Phe Val Thr Ala Ala Gly
GFP
1090 1100 1110
TTACACATGGCATGGATGAACTATACAAAT
AATGTGTACCGTACCTACTTGATATGTTTA
1110
Ile Thr His Gly Met Asp Glu Leu Tyr Lys
GFP
GFP reverse (1087 .. 1112)
1120 1130 1140
AGGGCGTAATCATGGTCATAGCTGTTTCCT
TCCCGCATTAGTACCAGTATCGACAAAGGA
1140
Pro Thr Ile Met Thr Met
lacZα
Ter
GFP
GFP-pUC19 backbone overlap
M13 rev
pUC19 backbone forward (1113 .. 1132)
GFP reverse (1087 .. 1112)
1150 1160 1170
GTGTGAAATTGTTATCCGCTCACAATTCCA
CACACTTTAACAATAGGCGAGTGTTAAGGT
1170
M13 rev
lac operator
Colony PCR reverse (1154 .. 1181)
1180 1190 1200
CACAACATACGAGCCGGAAGCATAAAGTGT
GTGTTGTATGCTCGGCCTTCGTATTTCACA
1200
lac promoter
Colony PCR reverse (1154 .. 1181)
1210 1220 1230
AAAGCCTGGGGTGCCTAATGAGTGAGCTAA
TTTCGGACCCCACGGATTACTCACTCGATT
1230
lac promoter
1240 1250 1260
CTCACATTAATTGCGTTGCGCTCACTGCCC
GAGTGTAATTAACGCAACGCGAGTGACGGG
1260
Sequencing reverse (1231 .. 1250)
1270 1280 1290
GCTTTCCAGTCGGGAAACCTGTCGTGCCAG
CGAAAGGTCAGCCCTTTGGACAGCACGGTC
1290
1300 1310 1320
CTGCATTAATGAATCGGCCAACGCGCGGGG
GACGTAATTACTTAGCCGGTTGCGCGCCCC
1320
1330 1340 1350
AGAGGCGGTTTGCGTATTGGGCGCTCTTCC
TCTCCGCCAAACGCATAACCCGCGAGAAGG
1350
1360 1370 1380
GCTTCCTCGCTCACTGACTCGCTGCGCTCG
CGAAGGAGCGAGTGACTGAGCGACGCGAGC
1380
1390 1400 1410
GTCGTTCGGCTGCGGCGAGCGGTATCAGCT
CAGCAAGCCGACGCCGCTCGCCATAGTCGA
1410
1420 1430 1440
CACTCAAAGGCGGTAATACGGTTATCCACA
GTGAGTTTCCGCCATTATGCCAATAGGTGT
1440
1450 1460 1470
GAATCAGGGGATAACGCAGGAAAGAACATG
CTTAGTCCCCTATTGCGTCCTTTCTTGTAC
1470
1480 1490 1500
TGAGCAAAAGGCCAGCAAAAGGCCAGGAAC
ACTCGTTTTCCGGTCGTTTTCCGGTCCTTG
1500
1510 1520 1530
CGTAAAAAGGCCGCGTTGCTGGCGTTTTTC
GCATTTTTCCGGCGCAACGACCGCAAAAAG
1530
ori
1540 1550 1560
CATAGGCTCCGCCCCCCTGACGAGCATCAC
GTATCCGAGGCGGGGGGACTGCTCGTAGTG
1560
ori
1570 1580 1590
AAAAATCGACGCTCAAGTCAGAGGTGGCGA
TTTTTAGCTGCGAGTTCAGTCTCCACCGCT
1590
ori
1600 1610 1620
AACCCGACAGGACTATAAAGATACCAGGCG
TTGGGCTGTCCTGATATTTCTATGGTCCGC
1620
ori
1630 1640 1650
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCT
AAAGGGGGACCTTCGAGGGAGCACGCGAGA
1650
ori
1660 1670 1680
CCTGTTCCGACCCTGCCGCTTACCGGATAC
GGACAAGGCTGGGACGGCGAATGGCCTATG
1680
ori
1690 1700 1710
CTGTCCGCCTTTCTCCCTTCGGGAAGCGTG
GACAGGCGGAAAGAGGGAAGCCCTTCGCAC
1710
ori
1720 1730 1740
GCGCTTTCTCATAGCTCACGCTGTAGGTAT
CGCGAAAGAGTATCGAGTGCGACATCCATA
1740
ori
1750 1760 1770
CTCAGTTCGGTGTAGGTCGTTCGCTCCAAG
GAGTCAAGCCACATCCAGCAAGCGAGGTTC
1770
ori
1780 1790 1800
CTGGGCTGTGTGCACGAACCCCCCGTTCAG
GACCCGACACACGTGCTTGGGGGGCAAGTC
1800
ori
1810 1820 1830
CCCGACCGCTGCGCCTTATCCGGTAACTAT
GGGCTGGCGACGCGGAATAGGCCATTGATA
1830
ori
1840 1850 1860
CGTCTTGAGTCCAACCCGGTAAGACACGAC
GCAGAACTCAGGTTGGGCCATTCTGTGCTG
1860
ori
1870 1880 1890
TTATCGCCACTGGCAGCAGCCACTGGTAAC
AATAGCGGTGACCGTCGTCGGTGACCATTG
1890
ori
1900 1910 1920
AGGATTAGCAGAGCGAGGTATGTAGGCGGT
TCCTAATCGTCTCGCTCCATACATCCGCCA
1920
ori
1930 1940 1950
GCTACAGAGTTCTTGAAGTGGTGGCCTAAC
CGATGTCTCAAGAACTTCACCACCGGATTG
1950
ori
1960 1970 1980
TACGGCTACACTAGAAGAACAGTATTTGGT
ATGCCGATGTGATCTTCTTGTCATAAACCA
1980
ori
1990 2000 2010
ATCTGCGCTCTGCTGAAGCCAGTTACCTTC
TAGACGCGAGACGACTTCGGTCAATGGAAG
2010
ori
2020 2030 2040
GGAAAAAGAGTTGGTAGCTCTTGATCCGGC
CCTTTTTCTCAACCATCGAGAACTAGGCCG
2040
ori
2050 2060 2070
AAACAAACCACCGCTGGTAGCGGTGGTTTT
TTTGTTTGGTGGCGACCATCGCCACCAAAA
2070
ori
2080 2090 2100
TTTGTTTGCAAGCAGCAGATTACGCGCAGA
AAACAAACGTTCGTCGTCTAATGCGCGTCT
2100
ori
2110 2120 2130
AAAAAAGGATCTCAAGAAGATCCTTTGATC
TTTTTTCCTAGAGTTCTTCTAGGAAACTAG
2130
ori
2140 2150 2160
TTTTCTACGGGGTCTGACGCTCAGTGGAAC
AAAAGATGCCCCAGACTGCGAGTCACCTTG
2160
2170 2180 2190
GAAAACTCACGTTAAGGGATTTTGGTCATG
CTTTTGAGTGCAATTCCCTAAAACCAGTAC
2190
2200 2210 2220
AGATTATCAAAAAGGATCTTCACCTAGATC
TCTAATAGTTTTTCCTAGAAGTGGATCTAG
2220
2230 2240 2250
CTTTTAAATTAAAAATGAAGTTTTAAATCA
GAAAATTTAATTTTTACTTCAAAATTTAGT
2250
2260 2270 2280
ATCTAAAGTATATATGAGTAAACTTGGTCT
TAGATTTCATATATACTCATTTGAACCAGA
2280
2290 2300 2310
GACAGTTACCAATGCTTAATCAGTGAGGCA
CTGTCAATGGTTACGAATTAGTCACTCCGT
2310
Trp His Lys Ile Leu Ser Ala
Ter
AmpR
2320 2330 2340
CCTATCTCAGCGATCTGTCTATTTCGTTCA
GGATAGAGTCGCTAGACAGATAAAGCAAGT
2340
Gly Ile Glu Ala Ile Gln Arg Asn Arg Glu
AmpR
2350 2360 2370
TCCATAGTTGCCTGACTCCCCGTCGTGTAG
AGGTATCAACGGACTGAGGGGCAGCACATC
2370
Asp Met Thr Ala Gln Ser Gly Thr Thr Tyr
AmpR
2380 2390 2400
ATAACTACGATACGGGAGGGCTTACCATCT
TATTGATGCTATGCCCTCCCGAATGGTAGA
2400
Ile Val Val Ile Arg Ser Pro Lys Gly Asp
AmpR
2410 2420 2430
GGCCCCAGTGCTGCAATGATACCGCGAGAC
CCGGGGTCACGACGTTACTATGGCGCTCTG
2430
Pro Gly Leu Ala Ala Ile Ile Gly Arg Ser
AmpR
2440 2450 2460
CCACGCTCACCGGCTCCAGATTTATCAGCA
GGTGCGAGTGGCCGAGGTCTAAATAGTCGT
2460
Gly Arg Glu Gly Ala Gly Ser Lys Asp Ala
AmpR
2470 2480 2490
ATAAACCAGCCAGCCGGAAGGGCCGAGCGC
TATTTGGTCGGTCGGCCTTCCCGGCTCGCG
2490
Ile Phe Trp Gly Ala Pro Leu Ala Ser Arg
AmpR
2500 2510 2520
AGAAGTGGTCCTGCAACTTTATCCGCCTCC
TCTTCACCAGGACGTTGAAATAGGCGGAGG
2520
Leu Leu Pro Gly Ala Val Lys Asp Ala Glu
AmpR
2530 2540 2550
ATCCAGTCTATTAATTGTTGCCGGGAAGCT
TAGGTCAGATAATTAACAACGGCCCTTCGA
2550
Met Trp Asp Ile Leu Gln Gln Arg Ser Ala
AmpR
2560 2570 2580
AGAGTAAGTAGTTCGCCAGTTAATAGTTTG
TCTCATTCATCAAGCGGTCAATTATCAAAC
2580
Leu Thr Leu Leu Glu Gly Thr Leu Leu Lys
AmpR
2590 2600 2610
CGCAACGTTGTTGCCATTGCTACAGGCATC
GCGTTGCAACAACGGTAACGATGTCCGTAG
2610
Arg Leu Thr Thr Ala Met Ala Val Pro Met
AmpR
2620 2630 2640
GTGGTGTCACGCTCGTCGTTTGGTATGGCT
CACCACAGTGCGAGCAGCAAACCATACCGA
2640
Thr Thr Asp Arg Glu Asp Asn Pro Ile Ala
AmpR
2650 2660 2670
TCATTCAGCTCCGGTTCCCAACGATCAAGG
AGTAAGTCGAGGCCAAGGGTTGCTAGTTCC
2670
Glu Asn Leu Glu Pro Glu Trp Arg Asp Leu
AmpR
2680 2690 2700
CGAGTTACATGATCCCCCATGTTGTGCAAA
GCTCAATGTACTAGGGGGTACAACACGTTT
2700
Arg Thr Val His Asp Gly Met Asn His Leu
AmpR
2710 2720 2730
AAAGCGGTTAGCTCCTTCGGTCCTCCGATC
TTTCGCCAATCGAGGAAGCCAGGAGGCTAG
2730
Phe Ala Thr Leu Glu Lys Pro Gly Gly Ile
AmpR
2740 2750 2760
GTTGTCAGAAGTAAGTTGGCCGCAGTGTTA
CAACAGTCTTCATTCAACCGGCGTCACAAT
2760
Thr Thr Leu Leu Leu Asn Ala Ala Thr Asn
AmpR
2770 2780 2790
TCACTCATGGTTATGGCAGCACTGCATAAT
AGTGAGTACCAATACCGTCGTGACGTATTA
2790
Asp Ser Met Thr Ile Ala Ala Ser Cys Leu
AmpR
2800 2810 2820
TCTCTTACTGTCATGCCATCCGTAAGATGC
AGAGAATGACAGTACGGTAGGCATTCTACG
2820
Glu Arg Val Thr Met Gly Asp Thr Leu His
AmpR
2830 2840 2850
TTTTCTGTGACTGGTGAGTACTCAACCAAG
AAAAGACACTGACCACTCATGAGTTGGTTC
2850
Lys Glu Thr Val Pro Ser Tyr Glu Val Leu
AmpR
2860 2870 2880
TCATTCTGAGAATAGTGTATGCGGCGACCG
AGTAAGACTCTTATCACATACGCCGCTGGC
2880
Asp Asn Gln Ser Tyr His Ile Arg Arg Gly
AmpR
2890 2900 2910
AGTTGCTCTTGCCCGGCGTCAATACGGGAT
TCAACGAGAACGGGCCGCAGTTATGCCCTA
2910
Leu Gln Glu Gln Gly Ala Asp Ile Arg Ser
AmpR
2920 2930 2940
AATACCGCGCCACATAGCAGAACTTTAAAA
TTATGGCGCGGTGTATCGTCTTGAAATTTT
2940
Leu Val Ala Gly Cys Leu Leu Val Lys Phe
AmpR
2950 2960 2970
GTGCTCATCATTGGAAAACGTTCTTCGGGG
CACGAGTAGTAACCTTTTGCAAGAAGCCCC
2970
Thr Ser Met Met Pro Phe Arg Glu Glu Pro
AmpR
2980 2990 3000
CGAAAACTCTCAAGGATCTTACCGCTGTTG
GCTTTTGAGAGTTCCTAGAATGGCGACAAC
3000
Arg Phe Ser Glu Leu Ile Lys Gly Ser Asn
AmpR
3010 3020 3030
AGATCCAGTTCGATGTAACCCACTCGTGCA
TCTAGGTCAAGCTACATTGGGTGAGCACGT
3030
Leu Asp Leu Glu Ile Tyr Gly Val Arg Ala
AmpR
3040 3050 3060
CCCAACTGATCTTCAGCATCTTTTACTTTC
GGGTTGACTAGAAGTCGTAGAAAATGAAAG
3060
Gly Leu Gln Asp Glu Ala Asp Lys Val Lys
AmpR
3070 3080 3090
ACCAGCGTTTCTGGGTGAGCAAAAACAGGA
TGGTCGCAAAGACCCACTCGTTTTTGTCCT
3090
Val Leu Thr Glu Pro His Ala Phe Val Pro
AmpR
3100 3110 3120
AGGCAAAATGCCGCAAAAAAGGGAATAAGG
TCCGTTTTACGGCGTTTTTTCCCTTATTCC
3120
Leu Cys Phe Ala Ala Phe Phe Pro Ile Leu
AmpR
3130 3140 3150
GCGACACGGAAATGTTGAATACTCATACTC
CGCTGTGCCTTTACAACTTATGAGTATGAG
3150
Ala Val Arg Phe His Gln Ile Ser Met
AmpR
AmpR promoter
3160 3170 3180
TTCCTTTTTCAATATTATTGAAGCATTTAT
AAGGAAAAAGTTATAATAACTTCGTAAATA
3180
AmpR promoter
3190 3200 3210
CAGGGTTATTGTCTCATGAGCGGATACATA
GTCCCAATAACAGAGTACTCGCCTATGTAT
3210
AmpR promoter
3220 3230 3240
TTTGAATGTATTTAGAAAAATAAACAAATA
AAACTTACATAAATCTTTTTATTTGTTTAT
3240
AmpR promoter
3250 3260 3270
GGGGTTCCGCGCACATTTCCCCGAAAAGTG
CCCCAAGGCGCGTGTAAAGGGGCTTTTCAC
3270
AmpR promoter
3280 3290 3300
CCACCTGACGTCTAAGAAACCATTATTATC
GGTGGACTGCAGATTCTTTGGTAATAATAG
3300
3310 3320 3330
ATGACATTAACCTATAAAAATAGGCGTATC
TACTGTAATTGGATATTTTTATCCGCATAG
3330
3340
ACGAGGCCCTTTCGTC
TGCTCCGGGAAAGCAG
3346