name	sequence	length	tm_c
pUC19 backbone forward	GGCGTAATCATGGTCATAGC	20	63.1
pUC19 backbone reverse	ACTGGCCGTCGTTTTACA	18	64.1
GFP forward	TAAAACGACGGCCAGTATGAGTAAAGGAGAAGAACTTTTCAC	42	62.1
GFP reverse	TGACCATGATTACGCCCTATTTGTATAGTTCATCCATGCCAT	42	62.0
Colony PCR forward	TGGGTAACGCCAGGGTTTTCC	21	61.9
Colony PCR reverse	CGTATGTTGTGTGGAATTGTGAGCGGAT	28	62.2
Junction reverse	CATCACCTTCACCCTCTCCACTGA	24	62.0
Sequencing forward	GCGCAACTGTTGGGAA	16	63.3
Sequencing reverse	CGCAACGCAATTAATGTGAG	20	62.2