name	sequence	length	tm_c
attB-GFP forward	GGGGACAAGTTTGTACAAAAAAGCAGGCTATGAGTAAAGGAGAAGAACTTTTCAC	55	62.1
attB-GFP reverse	GGGGACCACTTTGTACAAGAAAGCTGGGTCTATTTGTATAGTTCATCCATGCCAT	55	62.0
Colony PCR forward	AGGCTTGACCAATGCGTTCAGG	22	62.0
Colony PCR reverse	ATAGCCTGATGACCTTAGCAGTTCATGGA	29	62.0
Sequencing forward	GCTAAGGTCATCAGGCTATC	20	62.0
Sequencing reverse	TCGTATTAAGCCTGAACGC	19	62.0