Features
Cut sites
CDS
misc_feature
promoter
protein_bind
RBS
rep_origin
terminator
Circle
Line
Sequence
Zoom
Reset
1000
2000
3000
4000
5000
6000
N_JUN
f1 ori
KanR
ori
lacI
T7 promoter
lac operator
RBS
PmeI
N entry junction
DBD entry junction
DBD_JUN
DBD internal stuffer
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
KanR promoter
bom
rop
lacI promoter
BsaI
(370)
BbsI
(1315)
BbsI
(1345)
BsaI
(1379)
AP-1 DESynR round 2
6368 bp
1000
2000
3000
4000
5000
6000
N entry junction
N_JUN
N_JUN barcode
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
RBS
PmeI
DBD entry junction
DBD_JUN
DBD internal stuffer
DBD_JUN barcode
cloning scar
cloning scar
6xHis
PmeI
T7 terminator
BsaI
(370)
BbsI
(1315)
BbsI
(1345)
BsaI
(1379)
AP-1 DESynR round 2
6368 bp
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
5500
6000
N_JUN
DBD_JUN
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
RBS
PmeI
N entry junction
DBD entry junction
DBD internal stuffer
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
BsaI
(370)
BbsI
(1315)
BbsI
(1345)
BsaI
(1379)
AP-1 DESynR round 2
6368 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
3400
3600
3800
4000
4200
4400
4600
4800
5000
5200
5400
5600
5800
6000
6200
RBS
N_JUN
DBD entry junction
DBD_JUN
PmeI
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
PmeI
N entry junction
DBD internal stuffer
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
T7 terminator
BsaI
(370)
BbsI
(1315)
BbsI
(1345)
BsaI
(1379)
AP-1 DESynR round 2
6368 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
3400
3500
3600
3700
3800
3900
4000
4100
4200
4300
4400
4500
4600
4700
4800
4900
5000
5100
5200
5300
5400
5500
5600
5700
5800
5900
6000
6100
6200
6300
T7 promoter
lac operator
RBS
N_JUN
DBD entry junction
DBD_JUN
T7 terminator
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
PmeI
N entry junction
DBD internal stuffer
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
PmeI
BsaI
(370)
BbsI
(1315)
BbsI
(1345)
BsaI
(1379)
AP-1 DESynR round 2
6368 bp
Both strands
Copy
10 20 30 40 50 60
CAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGAT
GTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90 100 110 120
GGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCT
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150 160 170 180
CATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCC
GTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210 220 230 240
AGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGA
TCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270 280 290 300
GATCGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAA
CTAGCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330 340 350 360
CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAGTTTAAACTA
GTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGTCAAATTTGAT
360
lac operator
RBS
PmeI
370 380 390 400 410 420
TGGGGTCTCAAGGAATGACCGCCAAGATGGAAACCACCTTCTACGACGACGCCCTGAACG
ACCCCAGAGTTCCTTACTGGCGGTTCTACCTTTGGTGGAAGATGCTGCTGCGGGACTTGC
420
N entry junction
Met Thr Ala Lys Met Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn
N_JUN
BsaI
430 440 450 460 470 480
CCAGCTTCCTGCCCAGCGAGAGCGGCCCCTACGGCTACAGCAACCCCAAGATCCTGAAGC
GGTCGAAGGACGGGTCGCTCTCGCCGGGGATGCCGATGTCGTTGGGGTTCTAGGACTTCG
480
Ala Ser Phe Leu Pro Ser Glu Ser Gly Pro Tyr Gly Tyr Ser Asn Pro Lys Ile Leu Lys
N_JUN
490 500 510 520 530 540
AGAGCATGACCCTGAACCTGGCCGACCCCGTGGGCAGCCTGAAGCCCCACCTGAGAGCCA
TCTCGTACTGGGACTTGGACCGGCTGGGGCACCCGTCGGACTTCGGGGTGGACTCTCGGT
540
Gln Ser Met Thr Leu Asn Leu Ala Asp Pro Val Gly Ser Leu Lys Pro His Leu Arg Ala
N_JUN
550 560 570 580 590 600
AGAACAGCGACCTGCTGACCAGCCCCGACGTGGGCCTGCTGAAGCTGGCCAGCCCCGAGC
TCTTGTCGCTGGACGACTGGTCGGGGCTGCACCCGGACGACTTCGACCGGTCGGGGCTCG
600
Lys Asn Ser Asp Leu Leu Thr Ser Pro Asp Val Gly Leu Leu Lys Leu Ala Ser Pro Glu
N_JUN
610 620 630 640 650 660
TGGAGAGACTGATCATCCAGAGCAGCAACGGCCACATCACCACCACCCCCACCCCCACCC
ACCTCTCTGACTAGTAGGTCTCGTCGTTGCCGGTGTAGTGGTGGTGGGGGTGGGGGTGGG
660
Leu Glu Arg Leu Ile Ile Gln Ser Ser Asn Gly His Ile Thr Thr Thr Pro Thr Pro Thr
N_JUN
670 680 690 700 710 720
AGTTCCTGTGCCCCAAGAACGTGACCGACGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGA
TCAAGGACACGGGGTTCTTGCACTGGCTGCTCGTCCTCCCGAAGCGGCTCCCGAAGCACT
720
Gln Phe Leu Cys Pro Lys Asn Val Thr Asp Glu Gln Glu Gly Phe Ala Glu Gly Phe Val
N_JUN
730 740 750 760 770 780
GAGCCCTGGCCGAGCTGCACAGCCAGAACACCCTGCCCAGCGTGACCAGCGCCGCCCAGC
CTCGGGACCGGCTCGACGTGTCGGTCTTGTGGGACGGGTCGCACTGGTCGCGGCGGGTCG
780
Arg Ala Leu Ala Glu Leu His Ser Gln Asn Thr Leu Pro Ser Val Thr Ser Ala Ala Gln
N_JUN
790 800 810 820 830 840
CCGTGAACGGCGCCGGCATGGTGGCCCCCGCCGTGGCCAGCGTGGCCGGCGGCAGCGGCA
GGCACTTGCCGCGGCCGTACCACCGGGGGCGGCACCGGTCGCACCGGCCGCCGTCGCCGT
840
Pro Val Asn Gly Ala Gly Met Val Ala Pro Ala Val Ala Ser Val Ala Gly Gly Ser Gly
N_JUN
850 860 870 880 890 900
GCGGCGGCTTCAGCGCCAGCCTGCACAGCGAGCCCCCCGTGTACGCCAACCTGAGCAACT
CGCCGCCGAAGTCGCGGTCGGACGTGTCGCTCGGGGGGCACATGCGGTTGGACTCGTTGA
900
Ser Gly Gly Phe Ser Ala Ser Leu His Ser Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn
N_JUN
910 920 930 940 950 960
TCAACCCCGGCGCCCTGAGCAGCGGCGGCGGCGCCCCCAGCTACGGCGCCGCCGGCCTGG
AGTTGGGGCCGCGGGACTCGTCGCCGCCGCCGCGGGGGTCGATGCCGCGGCGGCCGGACC
960
Phe Asn Pro Gly Ala Leu Ser Ser Gly Gly Gly Ala Pro Ser Tyr Gly Ala Ala Gly Leu
N_JUN
970 980 990 1000 1010 1020
CCTTCCCCGCCCAGCCCCAGCAGCAGCAGCAGCCCCCCCACCATCTGCCCCAGCAGATGC
GGAAGGGGCGGGTCGGGGTCGTCGTCGTCGTCGGGGGGGTGGTAGACGGGGTCGTCTACG
1020
Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln Gln Pro Pro His His Leu Pro Gln Gln Met
N_JUN
1030 1040 1050 1060 1070 1080
CCGTGCAGCACCCCAGACTGCAGGCCCTGAAGGAGGAGCCCCAGACCGTGCCCGAGATGC
GGCACGTCGTGGGGTCTGACGTCCGGGACTTCCTCCTCGGGGTCTGGCACGGGCTCTACG
1080
Pro Val Gln His Pro Arg Leu Gln Ala Leu Lys Glu Glu Pro Gln Thr Val Pro Glu Met
N_JUN
1090 1100 1110 1120 1130 1140
CCGGCGAAACCCCCCCCCTGAGCCCCATCGACATGGAGAGCCAAGATAGAATCAAGGCCG
GGCCGCTTTGGGGGGGGGACTCGGGGTAGCTGTACCTCTCGGTTCTATCTTAGTTCCGGC
1140
Pro Gly Glu Thr Pro Pro Leu Ser Pro Ile Asp Met Glu Ser
N_JUN
DBD entry junction
Arg Ile Lys Ala
DBD_JUN
1150 1160 1170 1180 1190 1200
AGAGAAAGAGAATGAGAAACAGAATCGCCGCCAGCAAGTGCAGAAAGAGAAAGCTGGAGA
TCTCTTTCTCTTACTCTTTGTCTTAGCGGCGGTCGTTCACGTCTTTCTCTTTCGACCTCT
1200
Glu Arg Lys Arg Met Arg Asn Arg Ile Ala Ala Ser Lys Cys Arg Lys Arg Lys Leu Glu
DBD_JUN
1210 1220 1230 1240 1250 1260
GAATCGCCAGACTGGAGGAGAAGGTGAAAACCCTGAAGGCCCAGAACAGCGAGCTGGCCA
CTTAGCGGTCTGACCTCCTCTTCCACTTTTGGGACTTCCGGGTCTTGTCGCTCGACCGGT
1260
Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala
DBD_JUN
1270 1280 1290 1300 1310 1320
GCACCGCCAACATGCTGAGAGAACAGGTGGCCCAGCTGAAGCAGAAGGTGATGAAGCATA
CGTGGCGGTTGTACGACTCTCTTGTCCACCGGGTCGACTTCGTCTTCCACTACTTCGTAT
1320
Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Val Met
DBD_JUN
DBD internal stuffer
BbsI
1330 1340 1350 1360 1370 1380
AGTCTTCAGCCCGGGCAGAAGACAATTCCCACATAGTCGATTCCTACCATTGTCTTTCCT
TCAGAAGTCGGGCCCGTCTTCTGTTAAGGGTGTATCAGCTAAGGATGGTAACAGAAAGGA
1380
DBD internal stuffer
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
BbsI
BsaI
1390 1400 1410 1420 1430 1440
TCCGGAGACCACCACCACCACCACCACTGGTAGTTTAAACATGAGATCCGGCTGCTAACA
AGGCCTCTGGTGGTGGTGGTGGTGGTGACCATCAAATTTGTACTCTAGGCCGACGATTGT
1440
His His His His His His
6xHis
PmeI
1450 1460 1470 1480 1490 1500
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTCGGGCTTTCCTTCGACTCAACCGACGACGGTGGCGACTCGTTATTGATCGTATTGGGG
1500
T7 terminator
1510 1520 1530 1540 1550 1560
TTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT
AACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTA
1560
T7 terminator
1570 1580 1590 1600 1610 1620
TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCG
ACCGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGC
1620
f1 ori
1630 1640 1650 1660 1670 1680
CAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTC
GTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAG
1680
f1 ori
1690 1700 1710 1720 1730 1740
CTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGG
GAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCC
1740
f1 ori
1750 1760 1770 1780 1790 1800
GTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTC
CAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAG
1800
f1 ori
1810 1820 1830 1840 1850 1860
ACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT
TGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAA
1860
f1 ori
1870 1880 1890 1900 1910 1920
CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTC
GAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAG
1920
f1 ori
1930 1940 1950 1960 1970 1980
TTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTA
AAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAAT
1980
f1 ori
1990 2000 2010 2020 2030 2040
ACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTT
TGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGTCCACCGTGAAA
2040
f1 ori
2050 2060 2070 2080 2090 2100
TCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTA
AGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACAT
2100
2110 2120 2130 2140 2150 2160
TCCGCTCATGAATTAATTCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCT
AGGCGAGTACTTAATTAAGAGTCTTCTTGAGCAGTTCTTCCGCTATCTTCCGCTACGCGA
2160
Phe Phe Glu Asp Leu Leu Arg Tyr Phe Ala Ile Arg Gln
Ter
KanR
2170 2180 2190 2200 2210 2220
GCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAA
CGCTTAGCCCTCGCCGCTATGGCATTTCGTGCTCCTTCGCCAGTCGGGTAAGCGGCGGTT
2220
Ser Asp Pro Ala Ala Ile Gly Tyr Leu Val Leu Phe Arg Asp Ala Trp Glu Gly Gly Leu
KanR
2230 2240 2250 2260 2270 2280
GCTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCA
CGAGAAGTCGTTATAGTGCCCATCGGTTGCGATACAGGACTATCGCCAGGCGGTGTGGGT
2280
Glu Glu Ala Ile Asp Arg Thr Ala Leu Ala Ile Asp Gln Tyr Arg Asp Ala Val Gly Leu
KanR
2290 2300 2310 2320 2330 2340
GCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGC
CGGCCGGTGTCAGCTACTTAGGTCTTTTCGCCGGTAAAAGGTGGTACTATAAGCCGTTCG
2340
Arg Gly Cys Asp Ile Phe Gly Ser Phe Arg Gly Asn Glu Val Met Ile Asn Pro Leu Cys
KanR
2350 2360 2370 2380 2390 2400
AGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATGCTCGCCTTGAGCCTGG
TCCGTAGCGGTACCCAGTGCTGCTCTAGGAGCGGCAGCCCGTACGAGCGGAACTCGGACC
2400
Ala Asp Gly His Thr Val Val Leu Asp Glu Gly Asp Pro Met Ser Ala Lys Leu Arg Ala
KanR
2410 2420 2430 2440 2450 2460
CGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGATCGACAA
GCTTGTCAAGCCGACCGCGCTCGGGGACTACGAGAAGCAGGTCTAGTAGGACTAGCTGTT
2460
Phe Leu Glu Ala Pro Ala Leu Gly Gln His Glu Glu Asp Leu Asp Asp Gln Asp Val Leu
KanR
2470 2480 2490 2500 2510 2520
GACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATG
CTGGCCGAAGGTAGGCTCATGCACGAGCGAGCTACGCTACAAAGCGAACCACCAGCTTAC
2520
Gly Ala Glu Met Arg Thr Arg Ala Arg Glu Ile Arg His Lys Ala Gln His Asp Phe Pro
KanR
2530 2540 2550 2560 2570 2580
GGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTT
CCGTCCATCGGCCTAGTTCGCATACGTCGGCGGCGTAACGTAGTCGGTACTACCTATGAA
2580
Cys Thr Ala Pro Asp Leu Thr His Leu Arg Arg Met Ala Asp Ala Met Ile Ser Val Lys
KanR
2590 2600 2610 2620 2630 2640
TCTCGGCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCA
AGAGCCGTCCTCGTTCCACTCTACTGTCCTCTAGGACGGGGCCGTGAAGCGGGTTATCGT
2640
Glu Ala Pro Ala Leu His Ser Ser Leu Leu Asp Gln Gly Pro Val Glu Gly Leu Leu Leu
KanR
2650 2660 2670 2680 2690 2700
GCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCG
CGGTCAGGGAAGGGCGAAGTCACTGTTGCAGCTCGTGTCGACGCGTTCCTTGCGGGCAGC
2700
Trp Asp Arg Gly Ala Glu Thr Val Val Asp Leu Val Ala Ala Cys Pro Val Gly Thr Thr
KanR
2710 2720 2730 2740 2750 2760
TGGCCAGCCACGATAGCCGCGCTGCCTCGTCTTGCAGTTCATTCAGGGCACCGGACAGGT
ACCGGTCGGTGCTATCGGCGCGACGGAGCAGAACGTCAAGTAAGTCCCGTGGCCTGTCCA
2760
Ala Leu Trp Ser Leu Arg Ala Ala Glu Asp Gln Leu Glu Asn Leu Ala Gly Ser Leu Asp
KanR
2770 2780 2790 2800 2810 2820
CGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAG
GCCAGAACTGTTTTTCTTGGCCCGCGGGGACGCGACTGTCGGCCTTGTGCCGCCGTAGTC
2820
Thr Lys Val Phe Leu Val Pro Arg Gly Gln Ala Ser Leu Arg Phe Val Ala Ala Asp Ser
KanR
2830 2840 2850 2860 2870 2880
AGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCG
TCGTCGGCTAACAGACAACACGGGTCAGTATCGGCTTATCGGAGAGGTGGGTTCGCCGGC
2880
Cys Gly Ile Thr Gln Gln Ala Trp Asp Tyr Gly Phe Leu Arg Glu Val Trp Ala Ala Pro
KanR
2890 2900 2910 2920 2930 2940
GAGAACCTGCGTGCAATCCATCTTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAA
CTCTTGGACGCACGTTAGGTAGAACAAGTTAGTATGAGAAGGAAAAAGTTATAATAACTT
2940
Ser Gly Ala His Leu Gly Asp Gln Glu Ile Met
KanR
KanR promoter
2950 2960 2970 2980 2990 3000
GCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATA
CGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTAT
3000
KanR promoter
3010 3020 3030 3040 3050 3060
AACAAATAGGGGTTCCGCGAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTA
TTGTTTATCCCCAAGGCGCTTGTGGGGAACATAATGACAAATACATTCGTCTGTCAAAAT
3060
KanR promoter
3070 3080 3090 3100 3110 3120
TTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTA
AACAAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCAT
3120
3130 3140 3150 3160 3170 3180
GAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAA
CTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTT
3180
ori
3190 3200 3210 3220 3230 3240
ACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTT
TGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAA
3240
ori
3250 3260 3270 3280 3290 3300
TTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAG
AAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATC
3300
ori
3310 3320 3330 3340 3350 3360
CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTA
GGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGAT
3360
ori
3370 3380 3390 3400 3410 3420
ATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCA
TAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGT
3420
ori
3430 3440 3450 3460 3470 3480
AGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAG
TCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTC
3480
ori
3490 3500 3510 3520 3530 3540
CCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAA
GGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTT
3540
ori
3550 3560 3570 3580 3590 3600
AGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGA
TCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCT
3600
ori
3610 3620 3630 3640 3650 3660
ACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTC
TGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAG
3660
ori
3670 3680 3690 3700 3710 3720
GGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGC
CCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCG
3720
ori
3730 3740 3750 3760 3770 3780
CTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT
GATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAA
3780
ori
3790 3800 3810 3820 3830 3840
GCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT
CGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAA
3840
3850 3860 3870 3880 3890 3900
GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAG
CTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTC
3900
3910 3920 3930 3940 3950 3960
GAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACAC
CTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTG
3960
bom
3970 3980 3990 4000 4010 4020
CGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATA
GCGTATATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATAT
4020
bom
4030 4040 4050 4060 4070 4080
CACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGC
GTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCG
4080
bom
4090 4100 4110 4120 4130 4140
TGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGT
ACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCA
4140
4150 4160 4170 4180 4190 4200
CACCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCT
GTGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGA
4200
Leu Asn Glu Gly Asp Asp Gly Phe Arg Ala Leu Cys Ser
Ter
rop
4210 4220 4230 4240 4250 4260
GCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGC
CGCCATTTCGAGTAGTCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCG
4260
Arg Tyr Leu Glu Asp Ala His Asp His Leu Ser Glu Cys Ile Asp Ala Gln Glu Asp Ala
rop
4270 4280 4290 4300 4310 4320
GTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCAT
CAGGTCGAGCAACTCAAAGAGGTCTTCGCAATTACAGACCGAAGACTATTTCGCCCGGTA
4320
Asp Leu Glu Asn Leu Lys Glu Leu Leu Thr Leu Thr Gln Ser Arg Ile Phe Arg Ala Met
rop
4330 4340 4350 4360 4370 4380
GTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTT
CAATTCCCGCCAAAAAAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTAAAGACAA
4380
Asn Leu Ala Thr Lys Glu Gln Lys Thr Val
rop
4390 4400 4410 4420 4430 4440
CATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGA
GTACCCCCATTACTATGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAATGACTACT
4440
4450 4460 4470 4480 4490 4500
TGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCG
ACTTGTACGGGCCAATGACCTTGCAACACTCCCATTTGTTGACCGCCATACCTACGCCGC
4500
4510 4520 4530 4540 4550 4560
GGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGT
CCTGGTCTCTTTTTAGTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTACATCCACA
4560
4570 4580 4590 4600 4610 4620
TCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGC
AGGTGTCCCATCGGTCGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACGTCCCGCG
4620
4630 4640 4650 4660 4670 4680
TGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCAAAGACCATTCATGTTGTTGC
ACTGAAGGCGCAAAGGTCTGAAATGCTTTGTGCCTTTGGTTTCTGGTAAGTACAACAACG
4680
4690 4700 4710 4720 4730 4740
TCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTC
AGTCCAGCGTCTGCAAAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAG
4740
4750 4760 4770 4780 4790 4800
ATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCAC
TAAGACGATTGGTCATTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTG
4800
4810 4820 4830 4840 4850 4860
GATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACG
CTAGTACGCGTGGGCACCCCGGCGGTACGGCCGCTATTACCGGACGAAGAGCGGCTTTGC
4860
4870 4880 4890 4900 4910 4920
TTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGC
AAACCACCGCCCTGGTCACTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCG
4920
4930 4940 4950 4960 4970 4980
AAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCA
TTCGCTGTCCGGCTAGTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGT
4980
4990 5000 5010 5020 5030 5040
GAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAAAAGACAGTCATAAGTGCGGC
CTCGCGACGGCCGTGGACAGGATGCTCAACGTACTATTTTTTCTGTCAGTATTCACGCCG
5040
5050 5060 5070 5080 5090 5100
GACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGG
CTGCTATCAGTACGGGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCC
5100
5110 5120 5130 5140 5150 5160
CATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTC
GTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAATTAACGCAACGCGAG
5160
Ter
lacI
5170 5180 5190 5200 5210 5220
ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
TGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGC
5220
Gln Gly Ser Glu Leu Arg Ser Val Gln Arg Ala Leu Gln Met Leu Ser Asp Ala Leu Ala
lacI
5230 5240 5250 5260 5270 5280
CGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAAA
GCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTTT
5280
Arg Pro Ser Ala Thr Gln Thr Asn Pro Ala Leu Thr Thr Lys Arg Lys Val Leu Ser Val
lacI
5290 5300 5310 5320 5330 5340
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
GCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGT
5340
Pro Leu Leu Gln Asn Gly Lys Val Ala Gln Gly Gln Ser Leu Gln Leu Leu Arg Asp Val
lacI
5350 5360 5370 5380 5390 5400
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAAC
GCGACCAAACGGGGTCGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTG
5400
Ser Thr Gln Gly Leu Leu Arg Phe Asp Gln Lys Ile Thr Thr Leu Pro Pro Ile Tyr Cys
lacI
5410 5420 5430 5440 5450 5460
ATGAGCTATCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCC
TACTCGATAGAAGCCATAGCAGCATAGGGTGATGGCTCTACAGGCGTGGTTGCGCGTCGG
5460
Ser Ser Asp Glu Thr Asp Asp Tyr Gly Val Val Ser Ile Asp Ala Gly Val Arg Leu Gly
lacI
5470 5480 5490 5500 5510 5520
CGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCG
GCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGC
5520
Ser Glu Thr Ile Ala Arg Met Ala Gly Leu Ala Met Gln Asp Asn Ala Val Leu Met Ala
lacI
5530 5540 5550 5560 5570 5580
CAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCAC
GTCACCCTTGCTACGGGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTG
5580
Thr Pro Val Ile Gly Glu Asn Leu Met Gln Met Thr Gln Gln Phe Gly Ser Met Ala Ser
lacI
5590 5600 5610 5620 5630 5640
TCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCC
AGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGG
5640
Trp Asp Gly Glu Arg Glu Ala Ile Pro Gln Ile Gln Asn Arg Thr Leu Tyr Lys His Trp
lacI
5650 5660 5670 5680 5690 5700
AGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTT
TCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAA
5700
Gly Ala Leu Arg Leu Arg Ala Ser Val Ser Ser Leu Pro Gly Ala Leu Leu Ala Ile Gln
lacI
5710 5720 5730 5740 5750 5760
GCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCATCTTCATGGGAGA
CGACCACTGGGTTACGCTGGTCTACGAGGTGCGGGTCAGCGCATGGTAGAAGTACCCTCT
5760
Gln His Gly Leu Ala Val Leu His Glu Val Gly Leu Arg Thr Gly Asp Glu His Ser Phe
lacI
5770 5780 5790 5800 5810 5820
AAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAG
TTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATC
5820
Ile Ile Ser Asn Ile Pro Thr Gln Asp Ser Val Asp Leu Phe Leu Ala Pro Val Asn Thr
lacI
5830 5840 5850 5860 5870 5880
TGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCC
ACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGG
5880
Cys Ala Ala Glu Val Ala Ile Ala Asp Gln Asp Asp Leu Pro Tyr Asn Ile Ile Leu Gly
lacI
5890 5900 5910 5920 5930 5940
CACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTC
GTGACTGCGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAG
5940
Ser Val Arg Gln Ala Leu Leu Asn His Val Ala Ala Lys Cys Ala Glu Val Gly Ser Arg
lacI
5950 5960 5970 5980 5990 6000
GTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCG
CAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGC
6000
Glu Val Met Ser Val Val Val Ser Ala Gly Leu Gln Asp Ala Arg Ser Lys Ile Ala Ala
lacI
6010 6020 6030 6040 6050 6060
CGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACG
GCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGC
6060
Val Ile Gln Ser Pro Ala His Leu Ala Leu Ser Ser Thr Ala Val Gly Ile Leu Leu Ser
lacI
6070 6080 6090 6100 6110 6120
ACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCG
TGACAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGC
6120
Gln Lys Gly Ala Leu Gln Gln Ala Val Arg Asn Pro Ile Tyr Asn Leu Glu Ala Met Ala
lacI
6130 6140 6150 6160 6170 6180
CCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG
GGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCC
6180
Ala Glu Val Lys Glu Arg Thr Lys Ala Ser Val His Ser Ala Gln Asn Val Val Arg Ser
lacI
6190 6200 6210 6220 6230 6240
AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCA
TTTGCCAGACTATTCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGT
6240
Val Thr Gln Tyr Ser Val Gly Ala Tyr Glu Ala Val Asp Tyr Leu Thr Val Pro Lys Val
lacI
6250 6260 6270 6280 6290 6300
CATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTT
GTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAA
6300
lacI
lacI promoter
6310 6320 6330 6340 6350 6360
TGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGG
ACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCC
6360
lacI promoter
AAGCAGCC
TTCGTCGG
6368
10 20 30
CAGTAGTAGGTTGAGGCCGTTGAGCACCGC
GTCATCATCCAACTCCGGCAACTCGTGGCG
30
40 50 60
CGCCGCAAGGAATGGTGCATGCAAGGAGAT
GCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90
GGCGCCCAACAGTCCCCCGGCCACGGGGCC
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGG
90
100 110 120
TGCCACCATACCCACGCCGAAACAAGCGCT
ACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150
CATGAGCCCGAAGTGGCGAGCCCGATCTTC
GTACTCGGGCTTCACCGCTCGGGCTAGAAG
150
160 170 180
CCCATCGGTGATGTCGGCGATATAGGCGCC
GGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210
AGCAACCGCACCTGTGGCGCCGGTGATGCC
TCGTTGGCGTGGACACCGCGGCCACTACGG
210
220 230 240
GGCCACGATGCGTCCGGCGTAGAGGATCGA
CCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270
GATCGATCTCGATCCCGCGAAATTAATACG
CTAGCTAGAGCTAGGGCGCTTTAATTATGC
270
T7 promoter
280 290 300
ACTCACTATAGGGGAATTGTGAGCGGATAA
TGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330
CAATTCCCCTCTAGAAATAATTTTGTTTAA
GTTAAGGGGAGATCTTTATTAAAACAAATT
330
lac operator
RBS
340 350 360
CTTTAAGAAGGAGATATACAGTTTAAACTA
GAAATTCTTCCTCTATATGTCAAATTTGAT
360
RBS
PmeI
370 380 390
TGGGGTCTCAAGGAATGACCGCCAAGATGG
ACCCCAGAGTTCCTTACTGGCGGTTCTACC
390
N entry junction
Met Thr Ala Lys Met
N_JUN
BsaI
400 410 420
AAACCACCTTCTACGACGACGCCCTGAACG
TTTGGTGGAAGATGCTGCTGCGGGACTTGC
420
Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn
N_JUN
430 440 450
CCAGCTTCCTGCCCAGCGAGAGCGGCCCCT
GGTCGAAGGACGGGTCGCTCTCGCCGGGGA
450
Ala Ser Phe Leu Pro Ser Glu Ser Gly Pro
N_JUN
460 470 480
ACGGCTACAGCAACCCCAAGATCCTGAAGC
TGCCGATGTCGTTGGGGTTCTAGGACTTCG
480
Tyr Gly Tyr Ser Asn Pro Lys Ile Leu Lys
N_JUN
490 500 510
AGAGCATGACCCTGAACCTGGCCGACCCCG
TCTCGTACTGGGACTTGGACCGGCTGGGGC
510
Gln Ser Met Thr Leu Asn Leu Ala Asp Pro
N_JUN
520 530 540
TGGGCAGCCTGAAGCCCCACCTGAGAGCCA
ACCCGTCGGACTTCGGGGTGGACTCTCGGT
540
Val Gly Ser Leu Lys Pro His Leu Arg Ala
N_JUN
550 560 570
AGAACAGCGACCTGCTGACCAGCCCCGACG
TCTTGTCGCTGGACGACTGGTCGGGGCTGC
570
Lys Asn Ser Asp Leu Leu Thr Ser Pro Asp
N_JUN
580 590 600
TGGGCCTGCTGAAGCTGGCCAGCCCCGAGC
ACCCGGACGACTTCGACCGGTCGGGGCTCG
600
Val Gly Leu Leu Lys Leu Ala Ser Pro Glu
N_JUN
610 620 630
TGGAGAGACTGATCATCCAGAGCAGCAACG
ACCTCTCTGACTAGTAGGTCTCGTCGTTGC
630
Leu Glu Arg Leu Ile Ile Gln Ser Ser Asn
N_JUN
640 650 660
GCCACATCACCACCACCCCCACCCCCACCC
CGGTGTAGTGGTGGTGGGGGTGGGGGTGGG
660
Gly His Ile Thr Thr Thr Pro Thr Pro Thr
N_JUN
670 680 690
AGTTCCTGTGCCCCAAGAACGTGACCGACG
TCAAGGACACGGGGTTCTTGCACTGGCTGC
690
Gln Phe Leu Cys Pro Lys Asn Val Thr Asp
N_JUN
700 710 720
AGCAGGAGGGCTTCGCCGAGGGCTTCGTGA
TCGTCCTCCCGAAGCGGCTCCCGAAGCACT
720
Glu Gln Glu Gly Phe Ala Glu Gly Phe Val
N_JUN
730 740 750
GAGCCCTGGCCGAGCTGCACAGCCAGAACA
CTCGGGACCGGCTCGACGTGTCGGTCTTGT
750
Arg Ala Leu Ala Glu Leu His Ser Gln Asn
N_JUN
760 770 780
CCCTGCCCAGCGTGACCAGCGCCGCCCAGC
GGGACGGGTCGCACTGGTCGCGGCGGGTCG
780
Thr Leu Pro Ser Val Thr Ser Ala Ala Gln
N_JUN
790 800 810
CCGTGAACGGCGCCGGCATGGTGGCCCCCG
GGCACTTGCCGCGGCCGTACCACCGGGGGC
810
Pro Val Asn Gly Ala Gly Met Val Ala Pro
N_JUN
820 830 840
CCGTGGCCAGCGTGGCCGGCGGCAGCGGCA
GGCACCGGTCGCACCGGCCGCCGTCGCCGT
840
Ala Val Ala Ser Val Ala Gly Gly Ser Gly
N_JUN
850 860 870
GCGGCGGCTTCAGCGCCAGCCTGCACAGCG
CGCCGCCGAAGTCGCGGTCGGACGTGTCGC
870
Ser Gly Gly Phe Ser Ala Ser Leu His Ser
N_JUN
880 890 900
AGCCCCCCGTGTACGCCAACCTGAGCAACT
TCGGGGGGCACATGCGGTTGGACTCGTTGA
900
Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn
N_JUN
910 920 930
TCAACCCCGGCGCCCTGAGCAGCGGCGGCG
AGTTGGGGCCGCGGGACTCGTCGCCGCCGC
930
Phe Asn Pro Gly Ala Leu Ser Ser Gly Gly
N_JUN
940 950 960
GCGCCCCCAGCTACGGCGCCGCCGGCCTGG
CGCGGGGGTCGATGCCGCGGCGGCCGGACC
960
Gly Ala Pro Ser Tyr Gly Ala Ala Gly Leu
N_JUN
970 980 990
CCTTCCCCGCCCAGCCCCAGCAGCAGCAGC
GGAAGGGGCGGGTCGGGGTCGTCGTCGTCG
990
Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln
N_JUN
1000 1010 1020
AGCCCCCCCACCATCTGCCCCAGCAGATGC
TCGGGGGGGTGGTAGACGGGGTCGTCTACG
1020
Gln Pro Pro His His Leu Pro Gln Gln Met
N_JUN
1030 1040 1050
CCGTGCAGCACCCCAGACTGCAGGCCCTGA
GGCACGTCGTGGGGTCTGACGTCCGGGACT
1050
Pro Val Gln His Pro Arg Leu Gln Ala Leu
N_JUN
1060 1070 1080
AGGAGGAGCCCCAGACCGTGCCCGAGATGC
TCCTCCTCGGGGTCTGGCACGGGCTCTACG
1080
Lys Glu Glu Pro Gln Thr Val Pro Glu Met
N_JUN
1090 1100 1110
CCGGCGAAACCCCCCCCCTGAGCCCCATCG
GGCCGCTTTGGGGGGGGGACTCGGGGTAGC
1110
Pro Gly Glu Thr Pro Pro Leu Ser Pro Ile
N_JUN
1120 1130 1140
ACATGGAGAGCCAAGATAGAATCAAGGCCG
TGTACCTCTCGGTTCTATCTTAGTTCCGGC
1140
Asp Met Glu Ser
N_JUN
DBD entry junction
Arg Ile Lys Ala
DBD_JUN
1150 1160 1170
AGAGAAAGAGAATGAGAAACAGAATCGCCG
TCTCTTTCTCTTACTCTTTGTCTTAGCGGC
1170
Glu Arg Lys Arg Met Arg Asn Arg Ile Ala
DBD_JUN
1180 1190 1200
CCAGCAAGTGCAGAAAGAGAAAGCTGGAGA
GGTCGTTCACGTCTTTCTCTTTCGACCTCT
1200
Ala Ser Lys Cys Arg Lys Arg Lys Leu Glu
DBD_JUN
1210 1220 1230
GAATCGCCAGACTGGAGGAGAAGGTGAAAA
CTTAGCGGTCTGACCTCCTCTTCCACTTTT
1230
Arg Ile Ala Arg Leu Glu Glu Lys Val Lys
DBD_JUN
1240 1250 1260
CCCTGAAGGCCCAGAACAGCGAGCTGGCCA
GGGACTTCCGGGTCTTGTCGCTCGACCGGT
1260
Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala
DBD_JUN
1270 1280 1290
GCACCGCCAACATGCTGAGAGAACAGGTGG
CGTGGCGGTTGTACGACTCTCTTGTCCACC
1290
Ser Thr Ala Asn Met Leu Arg Glu Gln Val
DBD_JUN
1300 1310 1320
CCCAGCTGAAGCAGAAGGTGATGAAGCATA
GGGTCGACTTCGTCTTCCACTACTTCGTAT
1320
Ala Gln Leu Lys Gln Lys Val Met
DBD_JUN
DBD internal stuffer
BbsI
1330 1340 1350
AGTCTTCAGCCCGGGCAGAAGACAATTCCC
TCAGAAGTCGGGCCCGTCTTCTGTTAAGGG
1350
DBD internal stuffer
DBD_JUN barcode
BbsI
1360 1370 1380
ACATAGTCGATTCCTACCATTGTCTTTCCT
TGTATCAGCTAAGGATGGTAACAGAAAGGA
1380
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
BsaI
1390 1400 1410
TCCGGAGACCACCACCACCACCACCACTGG
AGGCCTCTGGTGGTGGTGGTGGTGGTGACC
1410
His His His His His His
6xHis
1420 1430 1440
TAGTTTAAACATGAGATCCGGCTGCTAACA
ATCAAATTTGTACTCTAGGCCGACGATTGT
1440
PmeI
1450 1460 1470
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
TTCGGGCTTTCCTTCGACTCAACCGACGAC
1470
1480 1490 1500
CCACCGCTGAGCAATAACTAGCATAACCCC
GGTGGCGACTCGTTATTGATCGTATTGGGG
1500
T7 terminator
1510 1520 1530
TTGGGGCCTCTAAACGGGTCTTGAGGGGTT
AACCCCGGAGATTTGCCCAGAACTCCCCAA
1530
T7 terminator
1540 1550 1560
TTTTGCTGAAAGGAGGAACTATATCCGGAT
AAAACGACTTTCCTCCTTGATATAGGCCTA
1560
T7 terminator
1570 1580 1590
TGGCGAATGGGACGCGCCCTGTAGCGGCGC
ACCGCTTACCCTGCGCGGGACATCGCCGCG
1590
f1 ori
1600 1610 1620
ATTAAGCGCGGCGGGTGTGGTGGTTACGCG
TAATTCGCGCCGCCCACACCACCAATGCGC
1620
f1 ori
1630 1640 1650
CAGCGTGACCGCTACACTTGCCAGCGCCCT
GTCGCACTGGCGATGTGAACGGTCGCGGGA
1650
f1 ori
1660 1670 1680
AGCGCCCGCTCCTTTCGCTTTCTTCCCTTC
TCGCGGGCGAGGAAAGCGAAAGAAGGGAAG
1680
f1 ori
1690 1700 1710
CTTTCTCGCCACGTTCGCCGGCTTTCCCCG
GAAAGAGCGGTGCAAGCGGCCGAAAGGGGC
1710
f1 ori
1720 1730 1740
TCAAGCTCTAAATCGGGGGCTCCCTTTAGG
AGTTCGAGATTTAGCCCCCGAGGGAAATCC
1740
f1 ori
1750 1760 1770
GTTCCGATTTAGTGCTTTACGGCACCTCGA
CAAGGCTAAATCACGAAATGCCGTGGAGCT
1770
f1 ori
1780 1790 1800
CCCCAAAAAACTTGATTAGGGTGATGGTTC
GGGGTTTTTTGAACTAATCCCACTACCAAG
1800
f1 ori
1810 1820 1830
ACGTAGTGGGCCATCGCCCTGATAGACGGT
TGCATCACCCGGTAGCGGGACTATCTGCCA
1830
f1 ori
1840 1850 1860
TTTTCGCCCTTTGACGTTGGAGTCCACGTT
AAAAGCGGGAAACTGCAACCTCAGGTGCAA
1860
f1 ori
1870 1880 1890
CTTTAATAGTGGACTCTTGTTCCAAACTGG
GAAATTATCACCTGAGAACAAGGTTTGACC
1890
f1 ori
1900 1910 1920
AACAACACTCAACCCTATCTCGGTCTATTC
TTGTTGTGAGTTGGGATAGAGCCAGATAAG
1920
f1 ori
1930 1940 1950
TTTTGATTTATAAGGGATTTTGCCGATTTC
AAAACTAAATATTCCCTAAAACGGCTAAAG
1950
f1 ori
1960 1970 1980
GGCCTATTGGTTAAAAAATGAGCTGATTTA
CCGGATAACCAATTTTTTACTCGACTAAAT
1980
f1 ori
1990 2000 2010
ACAAAAATTTAACGCGAATTTTAACAAAAT
TGTTTTTAAATTGCGCTTAAAATTGTTTTA
2010
f1 ori
2020 2030 2040
ATTAACGTTTACAATTTCAGGTGGCACTTT
TAATTGCAAATGTTAAAGTCCACCGTGAAA
2040
f1 ori
2050 2060 2070
TCGGGGAAATGTGCGCGGAACCCCTATTTG
AGCCCCTTTACACGCGCCTTGGGGATAAAC
2070
2080 2090 2100
TTTATTTTTCTAAATACATTCAAATATGTA
AAATAAAAAGATTTATGTAAGTTTATACAT
2100
2110 2120 2130
TCCGCTCATGAATTAATTCTCAGAAGAACT
AGGCGAGTACTTAATTAAGAGTCTTCTTGA
2130
Phe Phe Glu
Ter
KanR
2140 2150 2160
CGTCAAGAAGGCGATAGAAGGCGATGCGCT
GCAGTTCTTCCGCTATCTTCCGCTACGCGA
2160
Asp Leu Leu Arg Tyr Phe Ala Ile Arg Gln
KanR
2170 2180 2190
GCGAATCGGGAGCGGCGATACCGTAAAGCA
CGCTTAGCCCTCGCCGCTATGGCATTTCGT
2190
Ser Asp Pro Ala Ala Ile Gly Tyr Leu Val
KanR
2200 2210 2220
CGAGGAAGCGGTCAGCCCATTCGCCGCCAA
GCTCCTTCGCCAGTCGGGTAAGCGGCGGTT
2220
Leu Phe Arg Asp Ala Trp Glu Gly Gly Leu
KanR
2230 2240 2250
GCTCTTCAGCAATATCACGGGTAGCCAACG
CGAGAAGTCGTTATAGTGCCCATCGGTTGC
2250
Glu Glu Ala Ile Asp Arg Thr Ala Leu Ala
KanR
2260 2270 2280
CTATGTCCTGATAGCGGTCCGCCACACCCA
GATACAGGACTATCGCCAGGCGGTGTGGGT
2280
Ile Asp Gln Tyr Arg Asp Ala Val Gly Leu
KanR
2290 2300 2310
GCCGGCCACAGTCGATGAATCCAGAAAAGC
CGGCCGGTGTCAGCTACTTAGGTCTTTTCG
2310
Arg Gly Cys Asp Ile Phe Gly Ser Phe Arg
KanR
2320 2330 2340
GGCCATTTTCCACCATGATATTCGGCAAGC
CCGGTAAAAGGTGGTACTATAAGCCGTTCG
2340
Gly Asn Glu Val Met Ile Asn Pro Leu Cys
KanR
2350 2360 2370
AGGCATCGCCATGGGTCACGACGAGATCCT
TCCGTAGCGGTACCCAGTGCTGCTCTAGGA
2370
Ala Asp Gly His Thr Val Val Leu Asp Glu
KanR
2380 2390 2400
CGCCGTCGGGCATGCTCGCCTTGAGCCTGG
GCGGCAGCCCGTACGAGCGGAACTCGGACC
2400
Gly Asp Pro Met Ser Ala Lys Leu Arg Ala
KanR
2410 2420 2430
CGAACAGTTCGGCTGGCGCGAGCCCCTGAT
GCTTGTCAAGCCGACCGCGCTCGGGGACTA
2430
Phe Leu Glu Ala Pro Ala Leu Gly Gln His
KanR
2440 2450 2460
GCTCTTCGTCCAGATCATCCTGATCGACAA
CGAGAAGCAGGTCTAGTAGGACTAGCTGTT
2460
Glu Glu Asp Leu Asp Asp Gln Asp Val Leu
KanR
2470 2480 2490
GACCGGCTTCCATCCGAGTACGTGCTCGCT
CTGGCCGAAGGTAGGCTCATGCACGAGCGA
2490
Gly Ala Glu Met Arg Thr Arg Ala Arg Glu
KanR
2500 2510 2520
CGATGCGATGTTTCGCTTGGTGGTCGAATG
GCTACGCTACAAAGCGAACCACCAGCTTAC
2520
Ile Arg His Lys Ala Gln His Asp Phe Pro
KanR
2530 2540 2550
GGCAGGTAGCCGGATCAAGCGTATGCAGCC
CCGTCCATCGGCCTAGTTCGCATACGTCGG
2550
Cys Thr Ala Pro Asp Leu Thr His Leu Arg
KanR
2560 2570 2580
GCCGCATTGCATCAGCCATGATGGATACTT
CGGCGTAACGTAGTCGGTACTACCTATGAA
2580
Arg Met Ala Asp Ala Met Ile Ser Val Lys
KanR
2590 2600 2610
TCTCGGCAGGAGCAAGGTGAGATGACAGGA
AGAGCCGTCCTCGTTCCACTCTACTGTCCT
2610
Glu Ala Pro Ala Leu His Ser Ser Leu Leu
KanR
2620 2630 2640
GATCCTGCCCCGGCACTTCGCCCAATAGCA
CTAGGACGGGGCCGTGAAGCGGGTTATCGT
2640
Asp Gln Gly Pro Val Glu Gly Leu Leu Leu
KanR
2650 2660 2670
GCCAGTCCCTTCCCGCTTCAGTGACAACGT
CGGTCAGGGAAGGGCGAAGTCACTGTTGCA
2670
Trp Asp Arg Gly Ala Glu Thr Val Val Asp
KanR
2680 2690 2700
CGAGCACAGCTGCGCAAGGAACGCCCGTCG
GCTCGTGTCGACGCGTTCCTTGCGGGCAGC
2700
Leu Val Ala Ala Cys Pro Val Gly Thr Thr
KanR
2710 2720 2730
TGGCCAGCCACGATAGCCGCGCTGCCTCGT
ACCGGTCGGTGCTATCGGCGCGACGGAGCA
2730
Ala Leu Trp Ser Leu Arg Ala Ala Glu Asp
KanR
2740 2750 2760
CTTGCAGTTCATTCAGGGCACCGGACAGGT
GAACGTCAAGTAAGTCCCGTGGCCTGTCCA
2760
Gln Leu Glu Asn Leu Ala Gly Ser Leu Asp
KanR
2770 2780 2790
CGGTCTTGACAAAAAGAACCGGGCGCCCCT
GCCAGAACTGTTTTTCTTGGCCCGCGGGGA
2790
Thr Lys Val Phe Leu Val Pro Arg Gly Gln
KanR
2800 2810 2820
GCGCTGACAGCCGGAACACGGCGGCATCAG
CGCGACTGTCGGCCTTGTGCCGCCGTAGTC
2820
Ala Ser Leu Arg Phe Val Ala Ala Asp Ser
KanR
2830 2840 2850
AGCAGCCGATTGTCTGTTGTGCCCAGTCAT
TCGTCGGCTAACAGACAACACGGGTCAGTA
2850
Cys Gly Ile Thr Gln Gln Ala Trp Asp Tyr
KanR
2860 2870 2880
AGCCGAATAGCCTCTCCACCCAAGCGGCCG
TCGGCTTATCGGAGAGGTGGGTTCGCCGGC
2880
Gly Phe Leu Arg Glu Val Trp Ala Ala Pro
KanR
2890 2900 2910
GAGAACCTGCGTGCAATCCATCTTGTTCAA
CTCTTGGACGCACGTTAGGTAGAACAAGTT
2910
Ser Gly Ala His Leu Gly Asp Gln Glu Ile
KanR
2920 2930 2940
TCATACTCTTCCTTTTTCAATATTATTGAA
AGTATGAGAAGGAAAAAGTTATAATAACTT
2940
Met
KanR
KanR promoter
2950 2960 2970
GCATTTATCAGGGTTATTGTCTCATGAGCG
CGTAAATAGTCCCAATAACAGAGTACTCGC
2970
KanR promoter
2980 2990 3000
GATACATATTTGAATGTATTTAGAAAAATA
CTATGTATAAACTTACATAAATCTTTTTAT
3000
KanR promoter
3010 3020 3030
AACAAATAGGGGTTCCGCGAACACCCCTTG
TTGTTTATCCCCAAGGCGCTTGTGGGGAAC
3030
KanR promoter
3040 3050 3060
TATTACTGTTTATGTAAGCAGACAGTTTTA
ATAATGACAAATACATTCGTCTGTCAAAAT
3060
3070 3080 3090
TTGTTCATGACCAAAATCCCTTAACGTGAG
AACAAGTACTGGTTTTAGGGAATTGCACTC
3090
3100 3110 3120
TTTTCGTTCCACTGAGCGTCAGACCCCGTA
AAAAGCAAGGTGACTCGCAGTCTGGGGCAT
3120
3130 3140 3150
GAAAAGATCAAAGGATCTTCTTGAGATCCT
CTTTTCTAGTTTCCTAGAAGAACTCTAGGA
3150
ori
3160 3170 3180
TTTTTTCTGCGCGTAATCTGCTGCTTGCAA
AAAAAAGACGCGCATTAGACGACGAACGTT
3180
ori
3190 3200 3210
ACAAAAAAACCACCGCTACCAGCGGTGGTT
TGTTTTTTTGGTGGCGATGGTCGCCACCAA
3210
ori
3220 3230 3240
TGTTTGCCGGATCAAGAGCTACCAACTCTT
ACAAACGGCCTAGTTCTCGATGGTTGAGAA
3240
ori
3250 3260 3270
TTTCCGAAGGTAACTGGCTTCAGCAGAGCG
AAAGGCTTCCATTGACCGAAGTCGTCTCGC
3270
ori
3280 3290 3300
CAGATACCAAATACTGTCCTTCTAGTGTAG
GTCTATGGTTTATGACAGGAAGATCACATC
3300
ori
3310 3320 3330
CCGTAGTTAGGCCACCACTTCAAGAACTCT
GGCATCAATCCGGTGGTGAAGTTCTTGAGA
3330
ori
3340 3350 3360
GTAGCACCGCCTACATACCTCGCTCTGCTA
CATCGTGGCGGATGTATGGAGCGAGACGAT
3360
ori
3370 3380 3390
ATCCTGTTACCAGTGGCTGCTGCCAGTGGC
TAGGACAATGGTCACCGACGACGGTCACCG
3390
ori
3400 3410 3420
GATAAGTCGTGTCTTACCGGGTTGGACTCA
CTATTCAGCACAGAATGGCCCAACCTGAGT
3420
ori
3430 3440 3450
AGACGATAGTTACCGGATAAGGCGCAGCGG
TCTGCTATCAATGGCCTATTCCGCGTCGCC
3450
ori
3460 3470 3480
TCGGGCTGAACGGGGGGTTCGTGCACACAG
AGCCCGACTTGCCCCCCAAGCACGTGTGTC
3480
ori
3490 3500 3510
CCCAGCTTGGAGCGAACGACCTACACCGAA
GGGTCGAACCTCGCTTGCTGGATGTGGCTT
3510
ori
3520 3530 3540
CTGAGATACCTACAGCGTGAGCTATGAGAA
GACTCTATGGATGTCGCACTCGATACTCTT
3540
ori
3550 3560 3570
AGCGCCACGCTTCCCGAAGGGAGAAAGGCG
TCGCGGTGCGAAGGGCTTCCCTCTTTCCGC
3570
ori
3580 3590 3600
GACAGGTATCCGGTAAGCGGCAGGGTCGGA
CTGTCCATAGGCCATTCGCCGTCCCAGCCT
3600
ori
3610 3620 3630
ACAGGAGAGCGCACGAGGGAGCTTCCAGGG
TGTCCTCTCGCGTGCTCCCTCGAAGGTCCC
3630
ori
3640 3650 3660
GGAAACGCCTGGTATCTTTATAGTCCTGTC
CCTTTGCGGACCATAGAAATATCAGGACAG
3660
ori
3670 3680 3690
GGGTTTCGCCACCTCTGACTTGAGCGTCGA
CCCAAAGCGGTGGAGACTGAACTCGCAGCT
3690
ori
3700 3710 3720
TTTTTGTGATGCTCGTCAGGGGGGCGGAGC
AAAAACACTACGAGCAGTCCCCCCGCCTCG
3720
ori
3730 3740 3750
CTATGGAAAAACGCCAGCAACGCGGCCTTT
GATACCTTTTTGCGGTCGTTGCGCCGGAAA
3750
ori
3760 3770 3780
TTACGGTTCCTGGCCTTTTGCTGGCCTTTT
AATGCCAAGGACCGGAAAACGACCGGAAAA
3780
3790 3800 3810
GCTCACATGTTCTTTCCTGCGTTATCCCCT
CGAGTGTACAAGAAAGGACGCAATAGGGGA
3810
3820 3830 3840
GATTCTGTGGATAACCGTATTACCGCCTTT
CTAAGACACCTATTGGCATAATGGCGGAAA
3840
3850 3860 3870
GAGTGAGCTGATACCGCTCGCCGCAGCCGA
CTCACTCGACTATGGCGAGCGGCGTCGGCT
3870
3880 3890 3900
ACGACCGAGCGCAGCGAGTCAGTGAGCGAG
TGCTGGCTCGCGTCGCTCAGTCACTCGCTC
3900
3910 3920 3930
GAAGCGGAAGAGCGCCTGATGCGGTATTTT
CTTCGCCTTCTCGCGGACTACGCCATAAAA
3930
bom
3940 3950 3960
CTCCTTACGCATCTGTGCGGTATTTCACAC
GAGGAATGCGTAGACACGCCATAAAGTGTG
3960
bom
3970 3980 3990
CGCATATATGGTGCACTCTCAGTACAATCT
GCGTATATACCACGTGAGAGTCATGTTAGA
3990
bom
4000 4010 4020
GCTCTGATGCCGCATAGTTAAGCCAGTATA
CGAGACTACGGCGTATCAATTCGGTCATAT
4020
bom
4030 4040 4050
CACTCCGCTATCGCTACGTGACTGGGTCAT
GTGAGGCGATAGCGATGCACTGACCCAGTA
4050
bom
4060 4070 4080
GGCTGCGCCCCGACACCCGCCAACACCCGC
CCGACGCGGGGCTGTGGGCGGTTGTGGGCG
4080
bom
4090 4100 4110
TGACGCGCCCTGACGGGCTTGTCTGCTCCC
ACTGCGCGGGACTGCCCGAACAGACGAGGG
4110
4120 4130 4140
GGCATCCGCTTACAGACAAGCTGTGACCGT
CCGTAGGCGAATGTCTGTTCGACACTGGCA
4140
4150 4160 4170
CACCGGGAGCTGCATGTGTCAGAGGTTTTC
GTGGCCCTCGACGTACACAGTCTCCAAAAG
4170
Leu Asn Glu
Ter
rop
4180 4190 4200
ACCGTCATCACCGAAACGCGCGAGGCAGCT
TGGCAGTAGTGGCTTTGCGCGCTCCGTCGA
4200
Gly Asp Asp Gly Phe Arg Ala Leu Cys Ser
rop
4210 4220 4230
GCGGTAAAGCTCATCAGCGTGGTCGTGAAG
CGCCATTTCGAGTAGTCGCACCAGCACTTC
4230
Arg Tyr Leu Glu Asp Ala His Asp His Leu
rop
4240 4250 4260
CGATTCACAGATGTCTGCCTGTTCATCCGC
GCTAAGTGTCTACAGACGGACAAGTAGGCG
4260
Ser Glu Cys Ile Asp Ala Gln Glu Asp Ala
rop
4270 4280 4290
GTCCAGCTCGTTGAGTTTCTCCAGAAGCGT
CAGGTCGAGCAACTCAAAGAGGTCTTCGCA
4290
Asp Leu Glu Asn Leu Lys Glu Leu Leu Thr
rop
4300 4310 4320
TAATGTCTGGCTTCTGATAAAGCGGGCCAT
ATTACAGACCGAAGACTATTTCGCCCGGTA
4320
Leu Thr Gln Ser Arg Ile Phe Arg Ala Met
rop
4330 4340 4350
GTTAAGGGCGGTTTTTTCCTGTTTGGTCAC
CAATTCCCGCCAAAAAAGGACAAACCAGTG
4350
Asn Leu Ala Thr Lys Glu Gln Lys Thr Val
rop
4360 4370 4380
TGATGCCTCCGTGTAAGGGGGATTTCTGTT
ACTACGGAGGCACATTCCCCCTAAAGACAA
4380
4390 4400 4410
CATGGGGGTAATGATACCGATGAAACGAGA
GTACCCCCATTACTATGGCTACTTTGCTCT
4410
4420 4430 4440
GAGGATGCTCACGATACGGGTTACTGATGA
CTCCTACGAGTGCTATGCCCAATGACTACT
4440
4450 4460 4470
TGAACATGCCCGGTTACTGGAACGTTGTGA
ACTTGTACGGGCCAATGACCTTGCAACACT
4470
4480 4490 4500
GGGTAAACAACTGGCGGTATGGATGCGGCG
CCCATTTGTTGACCGCCATACCTACGCCGC
4500
4510 4520 4530
GGACCAGAGAAAAATCACTCAGGGTCAATG
CCTGGTCTCTTTTTAGTGAGTCCCAGTTAC
4530
4540 4550 4560
CCAGCGCTTCGTTAATACAGATGTAGGTGT
GGTCGCGAAGCAATTATGTCTACATCCACA
4560
4570 4580 4590
TCCACAGGGTAGCCAGCAGCATCCTGCGAT
AGGTGTCCCATCGGTCGTCGTAGGACGCTA
4590
4600 4610 4620
GCAGATCCGGAACATAATGGTGCAGGGCGC
CGTCTAGGCCTTGTATTACCACGTCCCGCG
4620
4630 4640 4650
TGACTTCCGCGTTTCCAGACTTTACGAAAC
ACTGAAGGCGCAAAGGTCTGAAATGCTTTG
4650
4660 4670 4680
ACGGAAACCAAAGACCATTCATGTTGTTGC
TGCCTTTGGTTTCTGGTAAGTACAACAACG
4680
4690 4700 4710
TCAGGTCGCAGACGTTTTGCAGCAGCAGTC
AGTCCAGCGTCTGCAAAACGTCGTCGTCAG
4710
4720 4730 4740
GCTTCACGTTCGCTCGCGTATCGGTGATTC
CGAAGTGCAAGCGAGCGCATAGCCACTAAG
4740
4750 4760 4770
ATTCTGCTAACCAGTAAGGCAACCCCGCCA
TAAGACGATTGGTCATTCCGTTGGGGCGGT
4770
4780 4790 4800
GCCTAGCCGGGTCCTCAACGACAGGAGCAC
CGGATCGGCCCAGGAGTTGCTGTCCTCGTG
4800
4810 4820 4830
GATCATGCGCACCCGTGGGGCCGCCATGCC
CTAGTACGCGTGGGCACCCCGGCGGTACGG
4830
4840 4850 4860
GGCGATAATGGCCTGCTTCTCGCCGAAACG
CCGCTATTACCGGACGAAGAGCGGCTTTGC
4860
4870 4880 4890
TTTGGTGGCGGGACCAGTGACGAAGGCTTG
AAACCACCGCCCTGGTCACTGCTTCCGAAC
4890
4900 4910 4920
AGCGAGGGCGTGCAAGATTCCGAATACCGC
TCGCTCCCGCACGTTCTAAGGCTTATGGCG
4920
4930 4940 4950
AAGCGACAGGCCGATCATCGTCGCGCTCCA
TTCGCTGTCCGGCTAGTAGCAGCGCGAGGT
4950
4960 4970 4980
GCGAAAGCGGTCCTCGCCGAAAATGACCCA
CGCTTTCGCCAGGAGCGGCTTTTACTGGGT
4980
4990 5000 5010
GAGCGCTGCCGGCACCTGTCCTACGAGTTG
CTCGCGACGGCCGTGGACAGGATGCTCAAC
5010
5020 5030 5040
CATGATAAAAAAGACAGTCATAAGTGCGGC
GTACTATTTTTTCTGTCAGTATTCACGCCG
5040
5050 5060 5070
GACGATAGTCATGCCCCGCGCCCACCGGAA
CTGCTATCAGTACGGGGCGCGGGTGGCCTT
5070
5080 5090 5100
GGAGCTGACTGGGTTGAAGGCTCTCAAGGG
CCTCGACTGACCCAACTTCCGAGAGTTCCC
5100
5110 5120 5130
CATCGGTCGAGATCCCGGTGCCTAATGAGT
GTAGCCAGCTCTAGGGCCACGGATTACTCA
5130
5140 5150 5160
GAGCTAACTTACATTAATTGCGTTGCGCTC
CTCGATTGAATGTAATTAACGCAACGCGAG
5160
Ter
lacI
5170 5180 5190
ACTGCCCGCTTTCCAGTCGGGAAACCTGTC
TGACGGGCGAAAGGTCAGCCCTTTGGACAG
5190
Gln Gly Ser Glu Leu Arg Ser Val Gln Arg
lacI
5200 5210 5220
GTGCCAGCTGCATTAATGAATCGGCCAACG
CACGGTCGACGTAATTACTTAGCCGGTTGC
5220
Ala Leu Gln Met Leu Ser Asp Ala Leu Ala
lacI
5230 5240 5250
CGCGGGGAGAGGCGGTTTGCGTATTGGGCG
GCGCCCCTCTCCGCCAAACGCATAACCCGC
5250
Arg Pro Ser Ala Thr Gln Thr Asn Pro Ala
lacI
5260 5270 5280
CCAGGGTGGTTTTTCTTTTCACCAGTGAAA
GGTCCCACCAAAAAGAAAAGTGGTCACTTT
5280
Leu Thr Thr Lys Arg Lys Val Leu Ser Val
lacI
5290 5300 5310
CGGGCAACAGCTGATTGCCCTTCACCGCCT
GCCCGTTGTCGACTAACGGGAAGTGGCGGA
5310
Pro Leu Leu Gln Asn Gly Lys Val Ala Gln
lacI
5320 5330 5340
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CCGGGACTCTCTCAACGTCGTTCGCCAGGT
5340
Gly Gln Ser Leu Gln Leu Leu Arg Asp Val
lacI
5350 5360 5370
CGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GCGACCAAACGGGGTCGTCCGCTTTTAGGA
5370
Ser Thr Gln Gly Leu Leu Arg Phe Asp Gln
lacI
5380 5390 5400
GTTTGATGGTGGTTAACGGCGGGATATAAC
CAAACTACCACCAATTGCCGCCCTATATTG
5400
Lys Ile Thr Thr Leu Pro Pro Ile Tyr Cys
lacI
5410 5420 5430
ATGAGCTATCTTCGGTATCGTCGTATCCCA
TACTCGATAGAAGCCATAGCAGCATAGGGT
5430
Ser Ser Asp Glu Thr Asp Asp Tyr Gly Val
lacI
5440 5450 5460
CTACCGAGATGTCCGCACCAACGCGCAGCC
GATGGCTCTACAGGCGTGGTTGCGCGTCGG
5460
Val Ser Ile Asp Ala Gly Val Arg Leu Gly
lacI
5470 5480 5490
CGGACTCGGTAATGGCGCGCATTGCGCCCA
GCCTGAGCCATTACCGCGCGTAACGCGGGT
5490
Ser Glu Thr Ile Ala Arg Met Ala Gly Leu
lacI
5500 5510 5520
GCGCCATCTGATCGTTGGCAACCAGCATCG
CGCGGTAGACTAGCAACCGTTGGTCGTAGC
5520
Ala Met Gln Asp Asn Ala Val Leu Met Ala
lacI
5530 5540 5550
CAGTGGGAACGATGCCCTCATTCAGCATTT
GTCACCCTTGCTACGGGAGTAAGTCGTAAA
5550
Thr Pro Val Ile Gly Glu Asn Leu Met Gln
lacI
5560 5570 5580
GCATGGTTTGTTGAAAACCGGACATGGCAC
CGTACCAAACAACTTTTGGCCTGTACCGTG
5580
Met Thr Gln Gln Phe Gly Ser Met Ala Ser
lacI
5590 5600 5610
TCCAGTCGCCTTCCCGTTCCGCTATCGGCT
AGGTCAGCGGAAGGGCAAGGCGATAGCCGA
5610
Trp Asp Gly Glu Arg Glu Ala Ile Pro Gln
lacI
5620 5630 5640
GAATTTGATTGCGAGTGAGATATTTATGCC
CTTAAACTAACGCTCACTCTATAAATACGG
5640
Ile Gln Asn Arg Thr Leu Tyr Lys His Trp
lacI
5650 5660 5670
AGCCAGCCAGACGCAGACGCGCCGAGACAG
TCGGTCGGTCTGCGTCTGCGCGGCTCTGTC
5670
Gly Ala Leu Arg Leu Arg Ala Ser Val Ser
lacI
5680 5690 5700
AACTTAATGGGCCCGCTAACAGCGCGATTT
TTGAATTACCCGGGCGATTGTCGCGCTAAA
5700
Ser Leu Pro Gly Ala Leu Leu Ala Ile Gln
lacI
5710 5720 5730
GCTGGTGACCCAATGCGACCAGATGCTCCA
CGACCACTGGGTTACGCTGGTCTACGAGGT
5730
Gln His Gly Leu Ala Val Leu His Glu Val
lacI
5740 5750 5760
CGCCCAGTCGCGTACCATCTTCATGGGAGA
GCGGGTCAGCGCATGGTAGAAGTACCCTCT
5760
Gly Leu Arg Thr Gly Asp Glu His Ser Phe
lacI
5770 5780 5790
AAATAATACTGTTGATGGGTGTCTGGTCAG
TTTATTATGACAACTACCCACAGACCAGTC
5790
Ile Ile Ser Asn Ile Pro Thr Gln Asp Ser
lacI
5800 5810 5820
AGACATCAAGAAATAACGCCGGAACATTAG
TCTGTAGTTCTTTATTGCGGCCTTGTAATC
5820
Val Asp Leu Phe Leu Ala Pro Val Asn Thr
lacI
5830 5840 5850
TGCAGGCAGCTTCCACAGCAATGGCATCCT
ACGTCCGTCGAAGGTGTCGTTACCGTAGGA
5850
Cys Ala Ala Glu Val Ala Ile Ala Asp Gln
lacI
5860 5870 5880
GGTCATCCAGCGGATAGTTAATGATCAGCC
CCAGTAGGTCGCCTATCAATTACTAGTCGG
5880
Asp Asp Leu Pro Tyr Asn Ile Ile Leu Gly
lacI
5890 5900 5910
CACTGACGCGTTGCGCGAGAAGATTGTGCA
GTGACTGCGCAACGCGCTCTTCTAACACGT
5910
Ser Val Arg Gln Ala Leu Leu Asn His Val
lacI
5920 5930 5940
CCGCCGCTTTACAGGCTTCGACGCCGCTTC
GGCGGCGAAATGTCCGAAGCTGCGGCGAAG
5940
Ala Ala Lys Cys Ala Glu Val Gly Ser Arg
lacI
5950 5960 5970
GTTCTACCATCGACACCACCACGCTGGCAC
CAAGATGGTAGCTGTGGTGGTGCGACCGTG
5970
Glu Val Met Ser Val Val Val Ser Ala Gly
lacI
5980 5990 6000
CCAGTTGATCGGCGCGAGATTTAATCGCCG
GGTCAACTAGCCGCGCTCTAAATTAGCGGC
6000
Leu Gln Asp Ala Arg Ser Lys Ile Ala Ala
lacI
6010 6020 6030
CGACAATTTGCGACGGCGCGTGCAGGGCCA
GCTGTTAAACGCTGCCGCGCACGTCCCGGT
6030
Val Ile Gln Ser Pro Ala His Leu Ala Leu
lacI
6040 6050 6060
GACTGGAGGTGGCAACGCCAATCAGCAACG
CTGACCTCCACCGTTGCGGTTAGTCGTTGC
6060
Ser Ser Thr Ala Val Gly Ile Leu Leu Ser
lacI
6070 6080 6090
ACTGTTTGCCCGCCAGTTGTTGTGCCACGC
TGACAAACGGGCGGTCAACAACACGGTGCG
6090
Gln Lys Gly Ala Leu Gln Gln Ala Val Arg
lacI
6100 6110 6120
GGTTGGGAATGTAATTCAGCTCCGCCATCG
CCAACCCTTACATTAAGTCGAGGCGGTAGC
6120
Asn Pro Ile Tyr Asn Leu Glu Ala Met Ala
lacI
6130 6140 6150
CCGCTTCCACTTTTTCCCGCGTTTTCGCAG
GGCGAAGGTGAAAAAGGGCGCAAAAGCGTC
6150
Ala Glu Val Lys Glu Arg Thr Lys Ala Ser
lacI
6160 6170 6180
AAACGTGGCTGGCCTGGTTCACCACGCGGG
TTTGCACCGACCGGACCAAGTGGTGCGCCC
6180
Val His Ser Ala Gln Asn Val Val Arg Ser
lacI
6190 6200 6210
AAACGGTCTGATAAGAGACACCGGCATACT
TTTGCCAGACTATTCTCTGTGGCCGTATGA
6210
Val Thr Gln Tyr Ser Val Gly Ala Tyr Glu
lacI
6220 6230 6240
CTGCGACATCGTATAACGTTACTGGTTTCA
GACGCTGTAGCATATTGCAATGACCAAAGT
6240
Ala Val Asp Tyr Leu Thr Val Pro Lys Val
lacI
6250 6260 6270
CATTCACCACCCTGAATTGACTCTCTTCCG
GTAAGTGGTGGGACTTAACTGAGAGAAGGC
6270
lacI
lacI promoter
6280 6290 6300
GGCGCTATCATGCCATACCGCGAAAGGTTT
CCGCGATAGTACGGTATGGCGCTTTCCAAA
6300
lacI promoter
6310 6320 6330
TGCGCCATTCGATGGTGTCCGGGATCTCGA
ACGCGGTAAGCTACCACAGGCCCTAGAGCT
6330
lacI promoter
6340 6350 6360
CGCTCTCCCTTATGCGACTCCTGCATTAGG
GCGAGAGGGAATACGCTGAGGACGTAATCC
6360
AAGCAGCC
TTCGTCGG
6368