Features
Cut sites
CDS
misc_feature
promoter
protein_bind
RBS
rep_origin
terminator
Circle
Line
Sequence
Zoom
Reset
1000
2000
3000
4000
5000
6000
N_JUN
f1 ori
KanR
ori
lacI
T7 promoter
lac operator
RBS
PmeI
N entry junction
N internal stuffer
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
KanR promoter
bom
rop
lacI promoter
BsaI
(370)
BbsI
(1123)
BbsI
(1153)
BsaI
(1172)
AP-1 DESynR round 1
6161 bp
1000
2000
3000
4000
5000
6000
N_JUN
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
RBS
PmeI
N entry junction
N internal stuffer
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
BsaI
(370)
BbsI
(1123)
BbsI
(1153)
BsaI
(1172)
AP-1 DESynR round 1
6161 bp
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
5500
6000
N_JUN
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
RBS
PmeI
N entry junction
N internal stuffer
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
BsaI
(370)
BbsI
(1123)
BbsI
(1153)
BsaI
(1172)
AP-1 DESynR round 1
6161 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
3400
3600
3800
4000
4200
4400
4600
4800
5000
5200
5400
5600
5800
6000
RBS
N_JUN
PmeI
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
PmeI
N entry junction
N internal stuffer
N_JUN barcode
cloning scar
6xHis
T7 terminator
BsaI
(370)
BbsI
(1123)
BbsI
(1153)
BsaI
(1172)
AP-1 DESynR round 1
6161 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
3400
3500
3600
3700
3800
3900
4000
4100
4200
4300
4400
4500
4600
4700
4800
4900
5000
5100
5200
5300
5400
5500
5600
5700
5800
5900
6000
6100
T7 promoter
lac operator
RBS
N_JUN
cloning scar
T7 terminator
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
PmeI
N entry junction
N internal stuffer
N_JUN barcode
6xHis
PmeI
BsaI
(370)
BbsI
(1123)
BbsI
(1153)
BsaI
(1172)
AP-1 DESynR round 1
6161 bp
Both strands
Copy
10 20 30 40 50 60
CAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGAT
GTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90 100 110 120
GGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCT
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150 160 170 180
CATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCC
GTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210 220 230 240
AGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGA
TCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270 280 290 300
GATCGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAA
CTAGCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330 340 350 360
CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAGTTTAAACTA
GTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGTCAAATTTGAT
360
lac operator
RBS
PmeI
370 380 390 400 410 420
TGGGGTCTCAAGGAATGACCGCCAAGATGGAAACCACCTTCTACGACGACGCCCTGAACG
ACCCCAGAGTTCCTTACTGGCGGTTCTACCTTTGGTGGAAGATGCTGCTGCGGGACTTGC
420
N entry junction
Met Thr Ala Lys Met Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn
N_JUN
BsaI
430 440 450 460 470 480
CCAGCTTCCTGCCCAGCGAGAGCGGCCCCTACGGCTACAGCAACCCCAAGATCCTGAAGC
GGTCGAAGGACGGGTCGCTCTCGCCGGGGATGCCGATGTCGTTGGGGTTCTAGGACTTCG
480
Ala Ser Phe Leu Pro Ser Glu Ser Gly Pro Tyr Gly Tyr Ser Asn Pro Lys Ile Leu Lys
N_JUN
490 500 510 520 530 540
AGAGCATGACCCTGAACCTGGCCGACCCCGTGGGCAGCCTGAAGCCCCACCTGAGAGCCA
TCTCGTACTGGGACTTGGACCGGCTGGGGCACCCGTCGGACTTCGGGGTGGACTCTCGGT
540
Gln Ser Met Thr Leu Asn Leu Ala Asp Pro Val Gly Ser Leu Lys Pro His Leu Arg Ala
N_JUN
550 560 570 580 590 600
AGAACAGCGACCTGCTGACCAGCCCCGACGTGGGCCTGCTGAAGCTGGCCAGCCCCGAGC
TCTTGTCGCTGGACGACTGGTCGGGGCTGCACCCGGACGACTTCGACCGGTCGGGGCTCG
600
Lys Asn Ser Asp Leu Leu Thr Ser Pro Asp Val Gly Leu Leu Lys Leu Ala Ser Pro Glu
N_JUN
610 620 630 640 650 660
TGGAGAGACTGATCATCCAGAGCAGCAACGGCCACATCACCACCACCCCCACCCCCACCC
ACCTCTCTGACTAGTAGGTCTCGTCGTTGCCGGTGTAGTGGTGGTGGGGGTGGGGGTGGG
660
Leu Glu Arg Leu Ile Ile Gln Ser Ser Asn Gly His Ile Thr Thr Thr Pro Thr Pro Thr
N_JUN
670 680 690 700 710 720
AGTTCCTGTGCCCCAAGAACGTGACCGACGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGA
TCAAGGACACGGGGTTCTTGCACTGGCTGCTCGTCCTCCCGAAGCGGCTCCCGAAGCACT
720
Gln Phe Leu Cys Pro Lys Asn Val Thr Asp Glu Gln Glu Gly Phe Ala Glu Gly Phe Val
N_JUN
730 740 750 760 770 780
GAGCCCTGGCCGAGCTGCACAGCCAGAACACCCTGCCCAGCGTGACCAGCGCCGCCCAGC
CTCGGGACCGGCTCGACGTGTCGGTCTTGTGGGACGGGTCGCACTGGTCGCGGCGGGTCG
780
Arg Ala Leu Ala Glu Leu His Ser Gln Asn Thr Leu Pro Ser Val Thr Ser Ala Ala Gln
N_JUN
790 800 810 820 830 840
CCGTGAACGGCGCCGGCATGGTGGCCCCCGCCGTGGCCAGCGTGGCCGGCGGCAGCGGCA
GGCACTTGCCGCGGCCGTACCACCGGGGGCGGCACCGGTCGCACCGGCCGCCGTCGCCGT
840
Pro Val Asn Gly Ala Gly Met Val Ala Pro Ala Val Ala Ser Val Ala Gly Gly Ser Gly
N_JUN
850 860 870 880 890 900
GCGGCGGCTTCAGCGCCAGCCTGCACAGCGAGCCCCCCGTGTACGCCAACCTGAGCAACT
CGCCGCCGAAGTCGCGGTCGGACGTGTCGCTCGGGGGGCACATGCGGTTGGACTCGTTGA
900
Ser Gly Gly Phe Ser Ala Ser Leu His Ser Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn
N_JUN
910 920 930 940 950 960
TCAACCCCGGCGCCCTGAGCAGCGGCGGCGGCGCCCCCAGCTACGGCGCCGCCGGCCTGG
AGTTGGGGCCGCGGGACTCGTCGCCGCCGCCGCGGGGGTCGATGCCGCGGCGGCCGGACC
960
Phe Asn Pro Gly Ala Leu Ser Ser Gly Gly Gly Ala Pro Ser Tyr Gly Ala Ala Gly Leu
N_JUN
970 980 990 1000 1010 1020
CCTTCCCCGCCCAGCCCCAGCAGCAGCAGCAGCCCCCCCACCATCTGCCCCAGCAGATGC
GGAAGGGGCGGGTCGGGGTCGTCGTCGTCGTCGGGGGGGTGGTAGACGGGGTCGTCTACG
1020
Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln Gln Pro Pro His His Leu Pro Gln Gln Met
N_JUN
1030 1040 1050 1060 1070 1080
CCGTGCAGCACCCCAGACTGCAGGCCCTGAAGGAGGAGCCCCAGACCGTGCCCGAGATGC
GGCACGTCGTGGGGTCTGACGTCCGGGACTTCCTCCTCGGGGTCTGGCACGGGCTCTACG
1080
Pro Val Gln His Pro Arg Leu Gln Ala Leu Lys Glu Glu Pro Gln Thr Val Pro Glu Met
N_JUN
1090 1100 1110 1120 1130 1140
CCGGCGAAACCCCCCCCCTGAGCCCCATCGACATGGAGAGCCAAGATAAGTCTTCAGCCC
GGCCGCTTTGGGGGGGGGACTCGGGGTAGCTGTACCTCTCGGTTCTATTCAGAAGTCGGG
1140
Pro Gly Glu Thr Pro Pro Leu Ser Pro Ile Asp Met Glu Ser
N_JUN
N internal stuffer
BbsI
1150 1160 1170 1180 1190 1200
GGGCAGAAGACAATTCCTACCATTGTCTTTCCTTCCGGAGACCACCACCACCACCACCAC
CCCGTCTTCTGTTAAGGATGGTAACAGAAAGGAAGGCCTCTGGTGGTGGTGGTGGTGGTG
1200
N internal stuffer
N_JUN barcode
cloning scar
His His His His His His
6xHis
BbsI
BsaI
1210 1220 1230 1240 1250 1260
TGGTAGTTTAAACATGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
ACCATCAAATTTGTACTCTAGGCCGACGATTGTTTCGGGCTTTCCTTCGACTCAACCGAC
1260
PmeI
1270 1280 1290 1300 1310 1320
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGG
GACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCC
1320
T7 terminator
1330 1340 1350 1360 1370 1380
GTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCGG
CAAAAAACGACTTTCCTCCTTGATATAGGCCTAACCGCTTACCCTGCGCGGGACATCGCC
1380
T7 terminator
f1 ori
1390 1400 1410 1420 1430 1440
CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGC
GCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCG
1440
f1 ori
1450 1460 1470 1480 1490 1500
CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCC
GGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGG
1500
f1 ori
1510 1520 1530 1540 1550 1560
CCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCT
GGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGA
1560
f1 ori
1570 1580 1590 1600 1610 1620
CGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC
GCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTG
1620
f1 ori
1630 1640 1650 1660 1670 1680
GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAAC
CCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTG
1680
f1 ori
1690 1700 1710 1720 1730 1740
TGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGAT
ACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTA
1740
f1 ori
1750 1760 1770 1780 1790 1800
TTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAA
AAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTT
1800
f1 ori
1810 1820 1830 1840 1850 1860
AATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTAT
TTATAATTGCAAATGTTAAAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATA
1860
f1 ori
1870 1880 1890 1900 1910 1920
TTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTCAGAAGA
AACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTTAATTAAGAGTCTTCT
1920
Phe Phe
Ter
KanR
1930 1940 1950 1960 1970 1980
ACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAA
TGAGCAGTTCTTCCGCTATCTTCCGCTACGCGACGCTTAGCCCTCGCCGCTATGGCATTT
1980
Glu Asp Leu Leu Arg Tyr Phe Ala Ile Arg Gln Ser Asp Pro Ala Ala Ile Gly Tyr Leu
KanR
1990 2000 2010 2020 2030 2040
GCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGGTAGCCA
CGTGCTCCTTCGCCAGTCGGGTAAGCGGCGGTTCGAGAAGTCGTTATAGTGCCCATCGGT
2040
Val Leu Phe Arg Asp Ala Trp Glu Gly Gly Leu Glu Glu Ala Ile Asp Arg Thr Ala Leu
KanR
2050 2060 2070 2080 2090 2100
ACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAATCCAGAAA
TGCGATACAGGACTATCGCCAGGCGGTGTGGGTCGGCCGGTGTCAGCTACTTAGGTCTTT
2100
Ala Ile Asp Gln Tyr Arg Asp Ala Val Gly Leu Arg Gly Cys Asp Ile Phe Gly Ser Phe
KanR
2110 2120 2130 2140 2150 2160
AGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGACGAGAT
TCGCCGGTAAAAGGTGGTACTATAAGCCGTTCGTCCGTAGCGGTACCCAGTGCTGCTCTA
2160
Arg Gly Asn Glu Val Met Ile Asn Pro Leu Cys Ala Asp Gly His Thr Val Val Leu Asp
KanR
2170 2180 2190 2200 2210 2220
CCTCGCCGTCGGGCATGCTCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCT
GGAGCGGCAGCCCGTACGAGCGGAACTCGGACCGCTTGTCAAGCCGACCGCGCTCGGGGA
2220
Glu Gly Asp Pro Met Ser Ala Lys Leu Arg Ala Phe Leu Glu Ala Pro Ala Leu Gly Gln
KanR
2230 2240 2250 2260 2270 2280
GATGCTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTC
CTACGAGAAGCAGGTCTAGTAGGACTAGCTGTTCTGGCCGAAGGTAGGCTCATGCACGAG
2280
His Glu Glu Asp Leu Asp Asp Gln Asp Val Leu Gly Ala Glu Met Arg Thr Arg Ala Arg
KanR
2290 2300 2310 2320 2330 2340
GCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGATCAAGCGTATGCA
CGAGCTACGCTACAAAGCGAACCACCAGCTTACCCGTCCATCGGCCTAGTTCGCATACGT
2340
Glu Ile Arg His Lys Ala Gln His Asp Phe Pro Cys Thr Ala Pro Asp Leu Thr His Leu
KanR
2350 2360 2370 2380 2390 2400
GCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTGAGATGACA
CGGCGGCGTAACGTAGTCGGTACTACCTATGAAAGAGCCGTCCTCGTTCCACTCTACTGT
2400
Arg Arg Met Ala Asp Ala Met Ile Ser Val Lys Glu Ala Pro Ala Leu His Ser Ser Leu
KanR
2410 2420 2430 2440 2450 2460
GGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAA
CCTCTAGGACGGGGCCGTGAAGCGGGTTATCGTCGGTCAGGGAAGGGCGAAGTCACTGTT
2460
Leu Asp Gln Gly Pro Val Glu Gly Leu Leu Leu Trp Asp Arg Gly Ala Glu Thr Val Val
KanR
2470 2480 2490 2500 2510 2520
CGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCT
GCAGCTCGTGTCGACGCGTTCCTTGCGGGCAGCACCGGTCGGTGCTATCGGCGCGACGGA
2520
Asp Leu Val Ala Ala Cys Pro Val Gly Thr Thr Ala Leu Trp Ser Leu Arg Ala Ala Glu
KanR
2530 2540 2550 2560 2570 2580
CGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCC
GCAGAACGTCAAGTAAGTCCCGTGGCCTGTCCAGCCAGAACTGTTTTTCTTGGCCCGCGG
2580
Asp Gln Leu Glu Asn Leu Ala Gly Ser Leu Asp Thr Lys Val Phe Leu Val Pro Arg Gly
KanR
2590 2600 2610 2620 2630 2640
CCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGT
GGACGCGACTGTCGGCCTTGTGCCGCCGTAGTCTCGTCGGCTAACAGACAACACGGGTCA
2640
Gln Ala Ser Leu Arg Phe Val Ala Ala Asp Ser Cys Gly Ile Thr Gln Gln Ala Trp Asp
KanR
2650 2660 2670 2680 2690 2700
CATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTT
GTATCGGCTTATCGGAGAGGTGGGTTCGCCGGCCTCTTGGACGCACGTTAGGTAGAACAA
2700
Tyr Gly Phe Leu Arg Glu Val Trp Ala Ala Pro Ser Gly Ala His Leu Gly Asp Gln Glu
KanR
2710 2720 2730 2740 2750 2760
CAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGA
GTTAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACT
2760
Ile Met
KanR
KanR promoter
2770 2780 2790 2800 2810 2820
GCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGAACACCCC
CGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCTTGTGGGG
2820
KanR promoter
2830 2840 2850 2860 2870 2880
TTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGT
AACATAATGACAAATACATTCGTCTGTCAAAATAACAAGTACTGGTTTTAGGGAATTGCA
2880
2890 2900 2910 2920 2930 2940
GAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGAT
CTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTA
2940
ori
2950 2960 2970 2980 2990 3000
CCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTG
GGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCAC
3000
ori
3010 3020 3030 3040 3050 3060
GTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGA
CAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCT
3060
ori
3070 3080 3090 3100 3110 3120
GCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAC
CGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTG
3120
ori
3130 3140 3150 3160 3170 3180
TCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT
AGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCA
3180
ori
3190 3200 3210 3220 3230 3240
GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAG
CCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTC
3240
ori
3250 3260 3270 3280 3290 3300
CGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACC
GCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGG
3300
ori
3310 3320 3330 3340 3350 3360
GAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAG
CTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTC
3360
ori
3370 3380 3390 3400 3410 3420
GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCA
CGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGT
3420
ori
3430 3440 3450 3460 3470 3480
GGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGT
CCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCA
3480
ori
3490 3500 3510 3520 3530 3540
CGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCC
GCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGG
3540
ori
3550 3560 3570 3580 3590 3600
TTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCC
AAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGG
3600
3610 3620 3630 3640 3650 3660
CCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGC
GGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCG
3660
3670 3680 3690 3700 3710 3720
CGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTAT
GCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCCATA
3720
bom
3730 3740 3750 3760 3770 3780
TTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAA
AAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATATACCACGTGAGAGTCATGTT
3780
bom
3790 3800 3810 3820 3830 3840
TCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGT
AGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGACCCA
3840
bom
3850 3860 3870 3880 3890 3900
CATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCT
GTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGA
3900
bom
3910 3920 3930 3940 3950 3960
CCCGGCATCCGCTTACAGACAAGCTGTGACCGTCACCGGGAGCTGCATGTGTCAGAGGTT
GGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGTGGCCCTCGACGTACACAGTCTCCAA
3960
Leu Asn
Ter
rop
3970 3980 3990 4000 4010 4020
TTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTG
AAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAGTCGCACCAGCAC
4020
Glu Gly Asp Asp Gly Phe Arg Ala Leu Cys Ser Arg Tyr Leu Glu Asp Ala His Asp His
rop
4030 4040 4050 4060 4070 4080
AAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAG
TTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGCAACTCAAAGAGGTCTTC
4080
Leu Ser Glu Cys Ile Asp Ala Gln Glu Asp Ala Asp Leu Glu Asn Leu Lys Glu Leu Leu
rop
4090 4100 4110 4120 4130 4140
CGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGT
GCAATTACAGACCGAAGACTATTTCGCCCGGTACAATTCCCGCCAAAAAAGGACAAACCA
4140
Thr Leu Thr Gln Ser Arg Ile Phe Arg Ala Met Asn Leu Ala Thr Lys Glu Gln Lys Thr
rop
4150 4160 4170 4180 4190 4200
CACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACG
GTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTATGGCTACTTTGC
4200
Val
rop
4210 4220 4230 4240 4250 4260
AGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTG
TCTCTCCTACGAGTGCTATGCCCAATGACTACTACTTGTACGGGCCAATGACCTTGCAAC
4260
4270 4280 4290 4300 4310 4320
TGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCA
ACTCCCATTTGTTGACCGCCATACCTACGCCGCCCTGGTCTCTTTTTAGTGAGTCCCAGT
4320
4330 4340 4350 4360 4370 4380
ATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGC
TACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGTCGTCGTAGGACG
4380
4390 4400 4410 4420 4430 4440
GATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGA
CTACGTCTAGGCCTTGTATTACCACGTCCCGCGACTGAAGGCGCAAAGGTCTGAAATGCT
4440
4450 4460 4470 4480 4490 4500
AACACGGAAACCAAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCA
TTGTGCCTTTGGTTTCTGGTAAGTACAACAACGAGTCCAGCGTCTGCAAAACGTCGTCGT
4500
4510 4520 4530 4540 4550 4560
GTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCG
CAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCATTCCGTTGGGGC
4560
4570 4580 4590 4600 4610 4620
CCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCAT
GGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTGCTAGTACGCGTGGGCACCCCGGCGGTA
4620
4630 4640 4650 4660 4670 4680
GCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGC
CGGCCGCTATTACCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGGTCACTGCTTCCG
4680
4690 4700 4710 4720 4730 4740
TTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCT
AACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTAGTAGCAGCGCGA
4740
4750 4760 4770 4780 4790 4800
CCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAG
GGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGTCTCGCGACGGCCGTGGACAGGATGCTC
4800
4810 4820 4830 4840 4850 4860
TTGCATGATAAAAAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCG
AACGTACTATTTTTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGGGGCGCGGGTGGC
4860
4870 4880 4890 4900 4910 4920
GAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATG
CTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGATTAC
4920
4930 4940 4950 4960 4970 4980
AGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCT
TCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGA
4980
Gln Gly Ser Glu Leu Arg Ser Val Gln
Ter
lacI
4990 5000 5010 5020 5030 5040
GTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGG
CAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACC
5040
Arg Ala Leu Gln Met Leu Ser Asp Ala Leu Ala Arg Pro Ser Ala Thr Gln Thr Asn Pro
lacI
5050 5060 5070 5080 5090 5100
GCGCCAGGGTGGTTTTTCTTTTCACCAGTGAAACGGGCAACAGCTGATTGCCCTTCACCG
CGCGGTCCCACCAAAAAGAAAAGTGGTCACTTTGCCCGTTGTCGACTAACGGGAAGTGGC
5100
Ala Leu Thr Thr Lys Arg Lys Val Leu Ser Val Pro Leu Leu Gln Asn Gly Lys Val Ala
lacI
5110 5120 5130 5140 5150 5160
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
GGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCTTTTA
5160
Gln Gly Gln Ser Leu Gln Leu Leu Arg Asp Val Ser Thr Gln Gly Leu Leu Arg Phe Asp
lacI
5170 5180 5190 5200 5210 5220
CCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTATCTTCGGTATCGTCGTATC
GGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGATAGAAGCCATAGCAGCATAG
5220
Gln Lys Ile Thr Thr Leu Pro Pro Ile Tyr Cys Ser Ser Asp Glu Thr Asp Asp Tyr Gly
lacI
5230 5240 5250 5260 5270 5280
CCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGC
GGTGATGGCTCTACAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAACGCG
5280
Val Val Ser Ile Asp Ala Gly Val Arg Leu Gly Ser Glu Thr Ile Ala Arg Met Ala Gly
lacI
5290 5300 5310 5320 5330 5340
CCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCA
GGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGAGTAAGTCGT
5340
Leu Ala Met Gln Asp Asn Ala Val Leu Met Ala Thr Pro Val Ile Gly Glu Asn Leu Met
lacI
5350 5360 5370 5380 5390 5400
TTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCG
AAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGC
5400
Gln Met Thr Gln Gln Phe Gly Ser Met Ala Ser Trp Asp Gly Glu Arg Glu Ala Ile Pro
lacI
5410 5420 5430 5440 5450 5460
GCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGA
CGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCT
5460
Gln Ile Gln Asn Arg Thr Leu Tyr Lys His Trp Gly Ala Leu Arg Leu Arg Ala Ser Val
lacI
5470 5480 5490 5500 5510 5520
CAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCT
GTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTACGCTGGTCTACGA
5520
Ser Ser Leu Pro Gly Ala Leu Leu Ala Ile Gln Gln His Gly Leu Ala Val Leu His Glu
lacI
5530 5540 5550 5560 5570 5580
CCACGCCCAGTCGCGTACCATCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGT
GGTGCGGGTCAGCGCATGGTAGAAGTACCCTCTTTTATTATGACAACTACCCACAGACCA
5580
Val Gly Leu Arg Thr Gly Asp Glu His Ser Phe Ile Ile Ser Asn Ile Pro Thr Gln Asp
lacI
5590 5600 5610 5620 5630 5640
CAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCAT
GTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACCGTA
5640
Ser Val Asp Leu Phe Leu Ala Pro Val Asn Thr Cys Ala Ala Glu Val Ala Ile Ala Asp
lacI
5650 5660 5670 5680 5690 5700
CCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGT
GGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGCAACGCGCTCTTCTAACA
5700
Gln Asp Asp Leu Pro Tyr Asn Ile Ile Leu Gly Ser Val Arg Gln Ala Leu Leu Asn His
lacI
5710 5720 5730 5740 5750 5760
GCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGG
CGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACC
5760
Val Ala Ala Lys Cys Ala Glu Val Gly Ser Arg Glu Val Met Ser Val Val Val Ser Ala
lacI
5770 5780 5790 5800 5810 5820
CACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGG
GTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCC
5820
Gly Leu Gln Asp Ala Arg Ser Lys Ile Ala Ala Val Ile Gln Ser Pro Ala His Leu Ala
lacI
5830 5840 5850 5860 5870 5880
CCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCA
GGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGGTCAACAACACGGT
5880
Leu Ser Ser Thr Ala Val Gly Ile Leu Leu Ser Gln Lys Gly Ala Leu Gln Gln Ala Val
lacI
5890 5900 5910 5920 5930 5940
CGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCG
GCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAAGGGCGCAAAAGC
5940
Arg Asn Pro Ile Tyr Asn Leu Glu Ala Met Ala Ala Glu Val Lys Glu Arg Thr Lys Ala
lacI
5950 5960 5970 5980 5990 6000
CAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCAT
GTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTA
6000
Ser Val His Ser Ala Gln Asn Val Val Arg Ser Val Thr Gln Tyr Ser Val Gly Ala Tyr
lacI
6010 6020 6030 6040 6050 6060
ACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTT
TGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACTTAACTGAGAGAA
6060
Glu Ala Val Asp Tyr Leu Thr Val Pro Lys Val
lacI
lacI promoter
6070 6080 6090 6100 6110 6120
CCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCT
GGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCTAGA
6120
lacI promoter
6130 6140 6150 6160
CGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCC
GCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGG
6161
10 20 30
CAGTAGTAGGTTGAGGCCGTTGAGCACCGC
GTCATCATCCAACTCCGGCAACTCGTGGCG
30
40 50 60
CGCCGCAAGGAATGGTGCATGCAAGGAGAT
GCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90
GGCGCCCAACAGTCCCCCGGCCACGGGGCC
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGG
90
100 110 120
TGCCACCATACCCACGCCGAAACAAGCGCT
ACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150
CATGAGCCCGAAGTGGCGAGCCCGATCTTC
GTACTCGGGCTTCACCGCTCGGGCTAGAAG
150
160 170 180
CCCATCGGTGATGTCGGCGATATAGGCGCC
GGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210
AGCAACCGCACCTGTGGCGCCGGTGATGCC
TCGTTGGCGTGGACACCGCGGCCACTACGG
210
220 230 240
GGCCACGATGCGTCCGGCGTAGAGGATCGA
CCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270
GATCGATCTCGATCCCGCGAAATTAATACG
CTAGCTAGAGCTAGGGCGCTTTAATTATGC
270
T7 promoter
280 290 300
ACTCACTATAGGGGAATTGTGAGCGGATAA
TGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330
CAATTCCCCTCTAGAAATAATTTTGTTTAA
GTTAAGGGGAGATCTTTATTAAAACAAATT
330
lac operator
RBS
340 350 360
CTTTAAGAAGGAGATATACAGTTTAAACTA
GAAATTCTTCCTCTATATGTCAAATTTGAT
360
RBS
PmeI
370 380 390
TGGGGTCTCAAGGAATGACCGCCAAGATGG
ACCCCAGAGTTCCTTACTGGCGGTTCTACC
390
N entry junction
Met Thr Ala Lys Met
N_JUN
BsaI
400 410 420
AAACCACCTTCTACGACGACGCCCTGAACG
TTTGGTGGAAGATGCTGCTGCGGGACTTGC
420
Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn
N_JUN
430 440 450
CCAGCTTCCTGCCCAGCGAGAGCGGCCCCT
GGTCGAAGGACGGGTCGCTCTCGCCGGGGA
450
Ala Ser Phe Leu Pro Ser Glu Ser Gly Pro
N_JUN
460 470 480
ACGGCTACAGCAACCCCAAGATCCTGAAGC
TGCCGATGTCGTTGGGGTTCTAGGACTTCG
480
Tyr Gly Tyr Ser Asn Pro Lys Ile Leu Lys
N_JUN
490 500 510
AGAGCATGACCCTGAACCTGGCCGACCCCG
TCTCGTACTGGGACTTGGACCGGCTGGGGC
510
Gln Ser Met Thr Leu Asn Leu Ala Asp Pro
N_JUN
520 530 540
TGGGCAGCCTGAAGCCCCACCTGAGAGCCA
ACCCGTCGGACTTCGGGGTGGACTCTCGGT
540
Val Gly Ser Leu Lys Pro His Leu Arg Ala
N_JUN
550 560 570
AGAACAGCGACCTGCTGACCAGCCCCGACG
TCTTGTCGCTGGACGACTGGTCGGGGCTGC
570
Lys Asn Ser Asp Leu Leu Thr Ser Pro Asp
N_JUN
580 590 600
TGGGCCTGCTGAAGCTGGCCAGCCCCGAGC
ACCCGGACGACTTCGACCGGTCGGGGCTCG
600
Val Gly Leu Leu Lys Leu Ala Ser Pro Glu
N_JUN
610 620 630
TGGAGAGACTGATCATCCAGAGCAGCAACG
ACCTCTCTGACTAGTAGGTCTCGTCGTTGC
630
Leu Glu Arg Leu Ile Ile Gln Ser Ser Asn
N_JUN
640 650 660
GCCACATCACCACCACCCCCACCCCCACCC
CGGTGTAGTGGTGGTGGGGGTGGGGGTGGG
660
Gly His Ile Thr Thr Thr Pro Thr Pro Thr
N_JUN
670 680 690
AGTTCCTGTGCCCCAAGAACGTGACCGACG
TCAAGGACACGGGGTTCTTGCACTGGCTGC
690
Gln Phe Leu Cys Pro Lys Asn Val Thr Asp
N_JUN
700 710 720
AGCAGGAGGGCTTCGCCGAGGGCTTCGTGA
TCGTCCTCCCGAAGCGGCTCCCGAAGCACT
720
Glu Gln Glu Gly Phe Ala Glu Gly Phe Val
N_JUN
730 740 750
GAGCCCTGGCCGAGCTGCACAGCCAGAACA
CTCGGGACCGGCTCGACGTGTCGGTCTTGT
750
Arg Ala Leu Ala Glu Leu His Ser Gln Asn
N_JUN
760 770 780
CCCTGCCCAGCGTGACCAGCGCCGCCCAGC
GGGACGGGTCGCACTGGTCGCGGCGGGTCG
780
Thr Leu Pro Ser Val Thr Ser Ala Ala Gln
N_JUN
790 800 810
CCGTGAACGGCGCCGGCATGGTGGCCCCCG
GGCACTTGCCGCGGCCGTACCACCGGGGGC
810
Pro Val Asn Gly Ala Gly Met Val Ala Pro
N_JUN
820 830 840
CCGTGGCCAGCGTGGCCGGCGGCAGCGGCA
GGCACCGGTCGCACCGGCCGCCGTCGCCGT
840
Ala Val Ala Ser Val Ala Gly Gly Ser Gly
N_JUN
850 860 870
GCGGCGGCTTCAGCGCCAGCCTGCACAGCG
CGCCGCCGAAGTCGCGGTCGGACGTGTCGC
870
Ser Gly Gly Phe Ser Ala Ser Leu His Ser
N_JUN
880 890 900
AGCCCCCCGTGTACGCCAACCTGAGCAACT
TCGGGGGGCACATGCGGTTGGACTCGTTGA
900
Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn
N_JUN
910 920 930
TCAACCCCGGCGCCCTGAGCAGCGGCGGCG
AGTTGGGGCCGCGGGACTCGTCGCCGCCGC
930
Phe Asn Pro Gly Ala Leu Ser Ser Gly Gly
N_JUN
940 950 960
GCGCCCCCAGCTACGGCGCCGCCGGCCTGG
CGCGGGGGTCGATGCCGCGGCGGCCGGACC
960
Gly Ala Pro Ser Tyr Gly Ala Ala Gly Leu
N_JUN
970 980 990
CCTTCCCCGCCCAGCCCCAGCAGCAGCAGC
GGAAGGGGCGGGTCGGGGTCGTCGTCGTCG
990
Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln
N_JUN
1000 1010 1020
AGCCCCCCCACCATCTGCCCCAGCAGATGC
TCGGGGGGGTGGTAGACGGGGTCGTCTACG
1020
Gln Pro Pro His His Leu Pro Gln Gln Met
N_JUN
1030 1040 1050
CCGTGCAGCACCCCAGACTGCAGGCCCTGA
GGCACGTCGTGGGGTCTGACGTCCGGGACT
1050
Pro Val Gln His Pro Arg Leu Gln Ala Leu
N_JUN
1060 1070 1080
AGGAGGAGCCCCAGACCGTGCCCGAGATGC
TCCTCCTCGGGGTCTGGCACGGGCTCTACG
1080
Lys Glu Glu Pro Gln Thr Val Pro Glu Met
N_JUN
1090 1100 1110
CCGGCGAAACCCCCCCCCTGAGCCCCATCG
GGCCGCTTTGGGGGGGGGACTCGGGGTAGC
1110
Pro Gly Glu Thr Pro Pro Leu Ser Pro Ile
N_JUN
1120 1130 1140
ACATGGAGAGCCAAGATAAGTCTTCAGCCC
TGTACCTCTCGGTTCTATTCAGAAGTCGGG
1140
Asp Met Glu Ser
N_JUN
N internal stuffer
BbsI
1150 1160 1170
GGGCAGAAGACAATTCCTACCATTGTCTTT
CCCGTCTTCTGTTAAGGATGGTAACAGAAA
1170
N internal stuffer
N_JUN barcode
cloning scar
BbsI
1180 1190 1200
CCTTCCGGAGACCACCACCACCACCACCAC
GGAAGGCCTCTGGTGGTGGTGGTGGTGGTG
1200
cloning scar
His His His His His His
6xHis
BsaI
1210 1220 1230
TGGTAGTTTAAACATGAGATCCGGCTGCTA
ACCATCAAATTTGTACTCTAGGCCGACGAT
1230
PmeI
1240 1250 1260
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
TGTTTCGGGCTTTCCTTCGACTCAACCGAC
1260
1270 1280 1290
CTGCCACCGCTGAGCAATAACTAGCATAAC
GACGGTGGCGACTCGTTATTGATCGTATTG
1290
T7 terminator
1300 1310 1320
CCCTTGGGGCCTCTAAACGGGTCTTGAGGG
GGGAACCCCGGAGATTTGCCCAGAACTCCC
1320
T7 terminator
1330 1340 1350
GTTTTTTGCTGAAAGGAGGAACTATATCCG
CAAAAAACGACTTTCCTCCTTGATATAGGC
1350
T7 terminator
1360 1370 1380
GATTGGCGAATGGGACGCGCCCTGTAGCGG
CTAACCGCTTACCCTGCGCGGGACATCGCC
1380
f1 ori
1390 1400 1410
CGCATTAAGCGCGGCGGGTGTGGTGGTTAC
GCGTAATTCGCGCCGCCCACACCACCAATG
1410
f1 ori
1420 1430 1440
GCGCAGCGTGACCGCTACACTTGCCAGCGC
CGCGTCGCACTGGCGATGTGAACGGTCGCG
1440
f1 ori
1450 1460 1470
CCTAGCGCCCGCTCCTTTCGCTTTCTTCCC
GGATCGCGGGCGAGGAAAGCGAAAGAAGGG
1470
f1 ori
1480 1490 1500
TTCCTTTCTCGCCACGTTCGCCGGCTTTCC
AAGGAAAGAGCGGTGCAAGCGGCCGAAAGG
1500
f1 ori
1510 1520 1530
CCGTCAAGCTCTAAATCGGGGGCTCCCTTT
GGCAGTTCGAGATTTAGCCCCCGAGGGAAA
1530
f1 ori
1540 1550 1560
AGGGTTCCGATTTAGTGCTTTACGGCACCT
TCCCAAGGCTAAATCACGAAATGCCGTGGA
1560
f1 ori
1570 1580 1590
CGACCCCAAAAAACTTGATTAGGGTGATGG
GCTGGGGTTTTTTGAACTAATCCCACTACC
1590
f1 ori
1600 1610 1620
TTCACGTAGTGGGCCATCGCCCTGATAGAC
AAGTGCATCACCCGGTAGCGGGACTATCTG
1620
f1 ori
1630 1640 1650
GGTTTTTCGCCCTTTGACGTTGGAGTCCAC
CCAAAAAGCGGGAAACTGCAACCTCAGGTG
1650
f1 ori
1660 1670 1680
GTTCTTTAATAGTGGACTCTTGTTCCAAAC
CAAGAAATTATCACCTGAGAACAAGGTTTG
1680
f1 ori
1690 1700 1710
TGGAACAACACTCAACCCTATCTCGGTCTA
ACCTTGTTGTGAGTTGGGATAGAGCCAGAT
1710
f1 ori
1720 1730 1740
TTCTTTTGATTTATAAGGGATTTTGCCGAT
AAGAAAACTAAATATTCCCTAAAACGGCTA
1740
f1 ori
1750 1760 1770
TTCGGCCTATTGGTTAAAAAATGAGCTGAT
AAGCCGGATAACCAATTTTTTACTCGACTA
1770
f1 ori
1780 1790 1800
TTAACAAAAATTTAACGCGAATTTTAACAA
AATTGTTTTTAAATTGCGCTTAAAATTGTT
1800
f1 ori
1810 1820 1830
AATATTAACGTTTACAATTTCAGGTGGCAC
TTATAATTGCAAATGTTAAAGTCCACCGTG
1830
f1 ori
1840 1850 1860
TTTTCGGGGAAATGTGCGCGGAACCCCTAT
AAAAGCCCCTTTACACGCGCCTTGGGGATA
1860
1870 1880 1890
TTGTTTATTTTTCTAAATACATTCAAATAT
AACAAATAAAAAGATTTATGTAAGTTTATA
1890
1900 1910 1920
GTATCCGCTCATGAATTAATTCTCAGAAGA
CATAGGCGAGTACTTAATTAAGAGTCTTCT
1920
Phe Phe
Ter
KanR
1930 1940 1950
ACTCGTCAAGAAGGCGATAGAAGGCGATGC
TGAGCAGTTCTTCCGCTATCTTCCGCTACG
1950
Glu Asp Leu Leu Arg Tyr Phe Ala Ile Arg
KanR
1960 1970 1980
GCTGCGAATCGGGAGCGGCGATACCGTAAA
CGACGCTTAGCCCTCGCCGCTATGGCATTT
1980
Gln Ser Asp Pro Ala Ala Ile Gly Tyr Leu
KanR
1990 2000 2010
GCACGAGGAAGCGGTCAGCCCATTCGCCGC
CGTGCTCCTTCGCCAGTCGGGTAAGCGGCG
2010
Val Leu Phe Arg Asp Ala Trp Glu Gly Gly
KanR
2020 2030 2040
CAAGCTCTTCAGCAATATCACGGGTAGCCA
GTTCGAGAAGTCGTTATAGTGCCCATCGGT
2040
Leu Glu Glu Ala Ile Asp Arg Thr Ala Leu
KanR
2050 2060 2070
ACGCTATGTCCTGATAGCGGTCCGCCACAC
TGCGATACAGGACTATCGCCAGGCGGTGTG
2070
Ala Ile Asp Gln Tyr Arg Asp Ala Val Gly
KanR
2080 2090 2100
CCAGCCGGCCACAGTCGATGAATCCAGAAA
GGTCGGCCGGTGTCAGCTACTTAGGTCTTT
2100
Leu Arg Gly Cys Asp Ile Phe Gly Ser Phe
KanR
2110 2120 2130
AGCGGCCATTTTCCACCATGATATTCGGCA
TCGCCGGTAAAAGGTGGTACTATAAGCCGT
2130
Arg Gly Asn Glu Val Met Ile Asn Pro Leu
KanR
2140 2150 2160
AGCAGGCATCGCCATGGGTCACGACGAGAT
TCGTCCGTAGCGGTACCCAGTGCTGCTCTA
2160
Cys Ala Asp Gly His Thr Val Val Leu Asp
KanR
2170 2180 2190
CCTCGCCGTCGGGCATGCTCGCCTTGAGCC
GGAGCGGCAGCCCGTACGAGCGGAACTCGG
2190
Glu Gly Asp Pro Met Ser Ala Lys Leu Arg
KanR
2200 2210 2220
TGGCGAACAGTTCGGCTGGCGCGAGCCCCT
ACCGCTTGTCAAGCCGACCGCGCTCGGGGA
2220
Ala Phe Leu Glu Ala Pro Ala Leu Gly Gln
KanR
2230 2240 2250
GATGCTCTTCGTCCAGATCATCCTGATCGA
CTACGAGAAGCAGGTCTAGTAGGACTAGCT
2250
His Glu Glu Asp Leu Asp Asp Gln Asp Val
KanR
2260 2270 2280
CAAGACCGGCTTCCATCCGAGTACGTGCTC
GTTCTGGCCGAAGGTAGGCTCATGCACGAG
2280
Leu Gly Ala Glu Met Arg Thr Arg Ala Arg
KanR
2290 2300 2310
GCTCGATGCGATGTTTCGCTTGGTGGTCGA
CGAGCTACGCTACAAAGCGAACCACCAGCT
2310
Glu Ile Arg His Lys Ala Gln His Asp Phe
KanR
2320 2330 2340
ATGGGCAGGTAGCCGGATCAAGCGTATGCA
TACCCGTCCATCGGCCTAGTTCGCATACGT
2340
Pro Cys Thr Ala Pro Asp Leu Thr His Leu
KanR
2350 2360 2370
GCCGCCGCATTGCATCAGCCATGATGGATA
CGGCGGCGTAACGTAGTCGGTACTACCTAT
2370
Arg Arg Met Ala Asp Ala Met Ile Ser Val
KanR
2380 2390 2400
CTTTCTCGGCAGGAGCAAGGTGAGATGACA
GAAAGAGCCGTCCTCGTTCCACTCTACTGT
2400
Lys Glu Ala Pro Ala Leu His Ser Ser Leu
KanR
2410 2420 2430
GGAGATCCTGCCCCGGCACTTCGCCCAATA
CCTCTAGGACGGGGCCGTGAAGCGGGTTAT
2430
Leu Asp Gln Gly Pro Val Glu Gly Leu Leu
KanR
2440 2450 2460
GCAGCCAGTCCCTTCCCGCTTCAGTGACAA
CGTCGGTCAGGGAAGGGCGAAGTCACTGTT
2460
Leu Trp Asp Arg Gly Ala Glu Thr Val Val
KanR
2470 2480 2490
CGTCGAGCACAGCTGCGCAAGGAACGCCCG
GCAGCTCGTGTCGACGCGTTCCTTGCGGGC
2490
Asp Leu Val Ala Ala Cys Pro Val Gly Thr
KanR
2500 2510 2520
TCGTGGCCAGCCACGATAGCCGCGCTGCCT
AGCACCGGTCGGTGCTATCGGCGCGACGGA
2520
Thr Ala Leu Trp Ser Leu Arg Ala Ala Glu
KanR
2530 2540 2550
CGTCTTGCAGTTCATTCAGGGCACCGGACA
GCAGAACGTCAAGTAAGTCCCGTGGCCTGT
2550
Asp Gln Leu Glu Asn Leu Ala Gly Ser Leu
KanR
2560 2570 2580
GGTCGGTCTTGACAAAAAGAACCGGGCGCC
CCAGCCAGAACTGTTTTTCTTGGCCCGCGG
2580
Asp Thr Lys Val Phe Leu Val Pro Arg Gly
KanR
2590 2600 2610
CCTGCGCTGACAGCCGGAACACGGCGGCAT
GGACGCGACTGTCGGCCTTGTGCCGCCGTA
2610
Gln Ala Ser Leu Arg Phe Val Ala Ala Asp
KanR
2620 2630 2640
CAGAGCAGCCGATTGTCTGTTGTGCCCAGT
GTCTCGTCGGCTAACAGACAACACGGGTCA
2640
Ser Cys Gly Ile Thr Gln Gln Ala Trp Asp
KanR
2650 2660 2670
CATAGCCGAATAGCCTCTCCACCCAAGCGG
GTATCGGCTTATCGGAGAGGTGGGTTCGCC
2670
Tyr Gly Phe Leu Arg Glu Val Trp Ala Ala
KanR
2680 2690 2700
CCGGAGAACCTGCGTGCAATCCATCTTGTT
GGCCTCTTGGACGCACGTTAGGTAGAACAA
2700
Pro Ser Gly Ala His Leu Gly Asp Gln Glu
KanR
2710 2720 2730
CAATCATACTCTTCCTTTTTCAATATTATT
GTTAGTATGAGAAGGAAAAAGTTATAATAA
2730
Ile Met
KanR
KanR promoter
2740 2750 2760
GAAGCATTTATCAGGGTTATTGTCTCATGA
CTTCGTAAATAGTCCCAATAACAGAGTACT
2760
KanR promoter
2770 2780 2790
GCGGATACATATTTGAATGTATTTAGAAAA
CGCCTATGTATAAACTTACATAAATCTTTT
2790
KanR promoter
2800 2810 2820
ATAAACAAATAGGGGTTCCGCGAACACCCC
TATTTGTTTATCCCCAAGGCGCTTGTGGGG
2820
KanR promoter
2830 2840 2850
TTGTATTACTGTTTATGTAAGCAGACAGTT
AACATAATGACAAATACATTCGTCTGTCAA
2850
2860 2870 2880
TTATTGTTCATGACCAAAATCCCTTAACGT
AATAACAAGTACTGGTTTTAGGGAATTGCA
2880
2890 2900 2910
GAGTTTTCGTTCCACTGAGCGTCAGACCCC
CTCAAAAGCAAGGTGACTCGCAGTCTGGGG
2910
2920 2930 2940
GTAGAAAAGATCAAAGGATCTTCTTGAGAT
CATCTTTTCTAGTTTCCTAGAAGAACTCTA
2940
ori
2950 2960 2970
CCTTTTTTTCTGCGCGTAATCTGCTGCTTG
GGAAAAAAAGACGCGCATTAGACGACGAAC
2970
ori
2980 2990 3000
CAAACAAAAAAACCACCGCTACCAGCGGTG
GTTTGTTTTTTTGGTGGCGATGGTCGCCAC
3000
ori
3010 3020 3030
GTTTGTTTGCCGGATCAAGAGCTACCAACT
CAAACAAACGGCCTAGTTCTCGATGGTTGA
3030
ori
3040 3050 3060
CTTTTTCCGAAGGTAACTGGCTTCAGCAGA
GAAAAAGGCTTCCATTGACCGAAGTCGTCT
3060
ori
3070 3080 3090
GCGCAGATACCAAATACTGTCCTTCTAGTG
CGCGTCTATGGTTTATGACAGGAAGATCAC
3090
ori
3100 3110 3120
TAGCCGTAGTTAGGCCACCACTTCAAGAAC
ATCGGCATCAATCCGGTGGTGAAGTTCTTG
3120
ori
3130 3140 3150
TCTGTAGCACCGCCTACATACCTCGCTCTG
AGACATCGTGGCGGATGTATGGAGCGAGAC
3150
ori
3160 3170 3180
CTAATCCTGTTACCAGTGGCTGCTGCCAGT
GATTAGGACAATGGTCACCGACGACGGTCA
3180
ori
3190 3200 3210
GGCGATAAGTCGTGTCTTACCGGGTTGGAC
CCGCTATTCAGCACAGAATGGCCCAACCTG
3210
ori
3220 3230 3240
TCAAGACGATAGTTACCGGATAAGGCGCAG
AGTTCTGCTATCAATGGCCTATTCCGCGTC
3240
ori
3250 3260 3270
CGGTCGGGCTGAACGGGGGGTTCGTGCACA
GCCAGCCCGACTTGCCCCCCAAGCACGTGT
3270
ori
3280 3290 3300
CAGCCCAGCTTGGAGCGAACGACCTACACC
GTCGGGTCGAACCTCGCTTGCTGGATGTGG
3300
ori
3310 3320 3330
GAACTGAGATACCTACAGCGTGAGCTATGA
CTTGACTCTATGGATGTCGCACTCGATACT
3330
ori
3340 3350 3360
GAAAGCGCCACGCTTCCCGAAGGGAGAAAG
CTTTCGCGGTGCGAAGGGCTTCCCTCTTTC
3360
ori
3370 3380 3390
GCGGACAGGTATCCGGTAAGCGGCAGGGTC
CGCCTGTCCATAGGCCATTCGCCGTCCCAG
3390
ori
3400 3410 3420
GGAACAGGAGAGCGCACGAGGGAGCTTCCA
CCTTGTCCTCTCGCGTGCTCCCTCGAAGGT
3420
ori
3430 3440 3450
GGGGGAAACGCCTGGTATCTTTATAGTCCT
CCCCCTTTGCGGACCATAGAAATATCAGGA
3450
ori
3460 3470 3480
GTCGGGTTTCGCCACCTCTGACTTGAGCGT
CAGCCCAAAGCGGTGGAGACTGAACTCGCA
3480
ori
3490 3500 3510
CGATTTTTGTGATGCTCGTCAGGGGGGCGG
GCTAAAAACACTACGAGCAGTCCCCCCGCC
3510
ori
3520 3530 3540
AGCCTATGGAAAAACGCCAGCAACGCGGCC
TCGGATACCTTTTTGCGGTCGTTGCGCCGG
3540
ori
3550 3560 3570
TTTTTACGGTTCCTGGCCTTTTGCTGGCCT
AAAAATGCCAAGGACCGGAAAACGACCGGA
3570
3580 3590 3600
TTTGCTCACATGTTCTTTCCTGCGTTATCC
AAACGAGTGTACAAGAAAGGACGCAATAGG
3600
3610 3620 3630
CCTGATTCTGTGGATAACCGTATTACCGCC
GGACTAAGACACCTATTGGCATAATGGCGG
3630
3640 3650 3660
TTTGAGTGAGCTGATACCGCTCGCCGCAGC
AAACTCACTCGACTATGGCGAGCGGCGTCG
3660
3670 3680 3690
CGAACGACCGAGCGCAGCGAGTCAGTGAGC
GCTTGCTGGCTCGCGTCGCTCAGTCACTCG
3690
3700 3710 3720
GAGGAAGCGGAAGAGCGCCTGATGCGGTAT
CTCCTTCGCCTTCTCGCGGACTACGCCATA
3720
bom
3730 3740 3750
TTTCTCCTTACGCATCTGTGCGGTATTTCA
AAAGAGGAATGCGTAGACACGCCATAAAGT
3750
bom
3760 3770 3780
CACCGCATATATGGTGCACTCTCAGTACAA
GTGGCGTATATACCACGTGAGAGTCATGTT
3780
bom
3790 3800 3810
TCTGCTCTGATGCCGCATAGTTAAGCCAGT
AGACGAGACTACGGCGTATCAATTCGGTCA
3810
bom
3820 3830 3840
ATACACTCCGCTATCGCTACGTGACTGGGT
TATGTGAGGCGATAGCGATGCACTGACCCA
3840
bom
3850 3860 3870
CATGGCTGCGCCCCGACACCCGCCAACACC
GTACCGACGCGGGGCTGTGGGCGGTTGTGG
3870
bom
3880 3890 3900
CGCTGACGCGCCCTGACGGGCTTGTCTGCT
GCGACTGCGCGGGACTGCCCGAACAGACGA
3900
3910 3920 3930
CCCGGCATCCGCTTACAGACAAGCTGTGAC
GGGCCGTAGGCGAATGTCTGTTCGACACTG
3930
3940 3950 3960
CGTCACCGGGAGCTGCATGTGTCAGAGGTT
GCAGTGGCCCTCGACGTACACAGTCTCCAA
3960
Leu Asn
Ter
rop
3970 3980 3990
TTCACCGTCATCACCGAAACGCGCGAGGCA
AAGTGGCAGTAGTGGCTTTGCGCGCTCCGT
3990
Glu Gly Asp Asp Gly Phe Arg Ala Leu Cys
rop
4000 4010 4020
GCTGCGGTAAAGCTCATCAGCGTGGTCGTG
CGACGCCATTTCGAGTAGTCGCACCAGCAC
4020
Ser Arg Tyr Leu Glu Asp Ala His Asp His
rop
4030 4040 4050
AAGCGATTCACAGATGTCTGCCTGTTCATC
TTCGCTAAGTGTCTACAGACGGACAAGTAG
4050
Leu Ser Glu Cys Ile Asp Ala Gln Glu Asp
rop
4060 4070 4080
CGCGTCCAGCTCGTTGAGTTTCTCCAGAAG
GCGCAGGTCGAGCAACTCAAAGAGGTCTTC
4080
Ala Asp Leu Glu Asn Leu Lys Glu Leu Leu
rop
4090 4100 4110
CGTTAATGTCTGGCTTCTGATAAAGCGGGC
GCAATTACAGACCGAAGACTATTTCGCCCG
4110
Thr Leu Thr Gln Ser Arg Ile Phe Arg Ala
rop
4120 4130 4140
CATGTTAAGGGCGGTTTTTTCCTGTTTGGT
GTACAATTCCCGCCAAAAAAGGACAAACCA
4140
Met Asn Leu Ala Thr Lys Glu Gln Lys Thr
rop
4150 4160 4170
CACTGATGCCTCCGTGTAAGGGGGATTTCT
GTGACTACGGAGGCACATTCCCCCTAAAGA
4170
Val
rop
4180 4190 4200
GTTCATGGGGGTAATGATACCGATGAAACG
CAAGTACCCCCATTACTATGGCTACTTTGC
4200
4210 4220 4230
AGAGAGGATGCTCACGATACGGGTTACTGA
TCTCTCCTACGAGTGCTATGCCCAATGACT
4230
4240 4250 4260
TGATGAACATGCCCGGTTACTGGAACGTTG
ACTACTTGTACGGGCCAATGACCTTGCAAC
4260
4270 4280 4290
TGAGGGTAAACAACTGGCGGTATGGATGCG
ACTCCCATTTGTTGACCGCCATACCTACGC
4290
4300 4310 4320
GCGGGACCAGAGAAAAATCACTCAGGGTCA
CGCCCTGGTCTCTTTTTAGTGAGTCCCAGT
4320
4330 4340 4350
ATGCCAGCGCTTCGTTAATACAGATGTAGG
TACGGTCGCGAAGCAATTATGTCTACATCC
4350
4360 4370 4380
TGTTCCACAGGGTAGCCAGCAGCATCCTGC
ACAAGGTGTCCCATCGGTCGTCGTAGGACG
4380
4390 4400 4410
GATGCAGATCCGGAACATAATGGTGCAGGG
CTACGTCTAGGCCTTGTATTACCACGTCCC
4410
4420 4430 4440
CGCTGACTTCCGCGTTTCCAGACTTTACGA
GCGACTGAAGGCGCAAAGGTCTGAAATGCT
4440
4450 4460 4470
AACACGGAAACCAAAGACCATTCATGTTGT
TTGTGCCTTTGGTTTCTGGTAAGTACAACA
4470
4480 4490 4500
TGCTCAGGTCGCAGACGTTTTGCAGCAGCA
ACGAGTCCAGCGTCTGCAAAACGTCGTCGT
4500
4510 4520 4530
GTCGCTTCACGTTCGCTCGCGTATCGGTGA
CAGCGAAGTGCAAGCGAGCGCATAGCCACT
4530
4540 4550 4560
TTCATTCTGCTAACCAGTAAGGCAACCCCG
AAGTAAGACGATTGGTCATTCCGTTGGGGC
4560
4570 4580 4590
CCAGCCTAGCCGGGTCCTCAACGACAGGAG
GGTCGGATCGGCCCAGGAGTTGCTGTCCTC
4590
4600 4610 4620
CACGATCATGCGCACCCGTGGGGCCGCCAT
GTGCTAGTACGCGTGGGCACCCCGGCGGTA
4620
4630 4640 4650
GCCGGCGATAATGGCCTGCTTCTCGCCGAA
CGGCCGCTATTACCGGACGAAGAGCGGCTT
4650
4660 4670 4680
ACGTTTGGTGGCGGGACCAGTGACGAAGGC
TGCAAACCACCGCCCTGGTCACTGCTTCCG
4680
4690 4700 4710
TTGAGCGAGGGCGTGCAAGATTCCGAATAC
AACTCGCTCCCGCACGTTCTAAGGCTTATG
4710
4720 4730 4740
CGCAAGCGACAGGCCGATCATCGTCGCGCT
GCGTTCGCTGTCCGGCTAGTAGCAGCGCGA
4740
4750 4760 4770
CCAGCGAAAGCGGTCCTCGCCGAAAATGAC
GGTCGCTTTCGCCAGGAGCGGCTTTTACTG
4770
4780 4790 4800
CCAGAGCGCTGCCGGCACCTGTCCTACGAG
GGTCTCGCGACGGCCGTGGACAGGATGCTC
4800
4810 4820 4830
TTGCATGATAAAAAAGACAGTCATAAGTGC
AACGTACTATTTTTTCTGTCAGTATTCACG
4830
4840 4850 4860
GGCGACGATAGTCATGCCCCGCGCCCACCG
CCGCTGCTATCAGTACGGGGCGCGGGTGGC
4860
4870 4880 4890
GAAGGAGCTGACTGGGTTGAAGGCTCTCAA
CTTCCTCGACTGACCCAACTTCCGAGAGTT
4890
4900 4910 4920
GGGCATCGGTCGAGATCCCGGTGCCTAATG
CCCGTAGCCAGCTCTAGGGCCACGGATTAC
4920
4930 4940 4950
AGTGAGCTAACTTACATTAATTGCGTTGCG
TCACTCGATTGAATGTAATTAACGCAACGC
4950
4960 4970 4980
CTCACTGCCCGCTTTCCAGTCGGGAAACCT
GAGTGACGGGCGAAAGGTCAGCCCTTTGGA
4980
Gln Gly Ser Glu Leu Arg Ser Val Gln
Ter
lacI
4990 5000 5010
GTCGTGCCAGCTGCATTAATGAATCGGCCA
CAGCACGGTCGACGTAATTACTTAGCCGGT
5010
Arg Ala Leu Gln Met Leu Ser Asp Ala Leu
lacI
5020 5030 5040
ACGCGCGGGGAGAGGCGGTTTGCGTATTGG
TGCGCGCCCCTCTCCGCCAAACGCATAACC
5040
Ala Arg Pro Ser Ala Thr Gln Thr Asn Pro
lacI
5050 5060 5070
GCGCCAGGGTGGTTTTTCTTTTCACCAGTG
CGCGGTCCCACCAAAAAGAAAAGTGGTCAC
5070
Ala Leu Thr Thr Lys Arg Lys Val Leu Ser
lacI
5080 5090 5100
AAACGGGCAACAGCTGATTGCCCTTCACCG
TTTGCCCGTTGTCGACTAACGGGAAGTGGC
5100
Val Pro Leu Leu Gln Asn Gly Lys Val Ala
lacI
5110 5120 5130
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
GGACCGGGACTCTCTCAACGTCGTTCGCCA
5130
Gln Gly Gln Ser Leu Gln Leu Leu Arg Asp
lacI
5140 5150 5160
CCACGCTGGTTTGCCCCAGCAGGCGAAAAT
GGTGCGACCAAACGGGGTCGTCCGCTTTTA
5160
Val Ser Thr Gln Gly Leu Leu Arg Phe Asp
lacI
5170 5180 5190
CCTGTTTGATGGTGGTTAACGGCGGGATAT
GGACAAACTACCACCAATTGCCGCCCTATA
5190
Gln Lys Ile Thr Thr Leu Pro Pro Ile Tyr
lacI
5200 5210 5220
AACATGAGCTATCTTCGGTATCGTCGTATC
TTGTACTCGATAGAAGCCATAGCAGCATAG
5220
Cys Ser Ser Asp Glu Thr Asp Asp Tyr Gly
lacI
5230 5240 5250
CCACTACCGAGATGTCCGCACCAACGCGCA
GGTGATGGCTCTACAGGCGTGGTTGCGCGT
5250
Val Val Ser Ile Asp Ala Gly Val Arg Leu
lacI
5260 5270 5280
GCCCGGACTCGGTAATGGCGCGCATTGCGC
CGGGCCTGAGCCATTACCGCGCGTAACGCG
5280
Gly Ser Glu Thr Ile Ala Arg Met Ala Gly
lacI
5290 5300 5310
CCAGCGCCATCTGATCGTTGGCAACCAGCA
GGTCGCGGTAGACTAGCAACCGTTGGTCGT
5310
Leu Ala Met Gln Asp Asn Ala Val Leu Met
lacI
5320 5330 5340
TCGCAGTGGGAACGATGCCCTCATTCAGCA
AGCGTCACCCTTGCTACGGGAGTAAGTCGT
5340
Ala Thr Pro Val Ile Gly Glu Asn Leu Met
lacI
5350 5360 5370
TTTGCATGGTTTGTTGAAAACCGGACATGG
AAACGTACCAAACAACTTTTGGCCTGTACC
5370
Gln Met Thr Gln Gln Phe Gly Ser Met Ala
lacI
5380 5390 5400
CACTCCAGTCGCCTTCCCGTTCCGCTATCG
GTGAGGTCAGCGGAAGGGCAAGGCGATAGC
5400
Ser Trp Asp Gly Glu Arg Glu Ala Ile Pro
lacI
5410 5420 5430
GCTGAATTTGATTGCGAGTGAGATATTTAT
CGACTTAAACTAACGCTCACTCTATAAATA
5430
Gln Ile Gln Asn Arg Thr Leu Tyr Lys His
lacI
5440 5450 5460
GCCAGCCAGCCAGACGCAGACGCGCCGAGA
CGGTCGGTCGGTCTGCGTCTGCGCGGCTCT
5460
Trp Gly Ala Leu Arg Leu Arg Ala Ser Val
lacI
5470 5480 5490
CAGAACTTAATGGGCCCGCTAACAGCGCGA
GTCTTGAATTACCCGGGCGATTGTCGCGCT
5490
Ser Ser Leu Pro Gly Ala Leu Leu Ala Ile
lacI
5500 5510 5520
TTTGCTGGTGACCCAATGCGACCAGATGCT
AAACGACCACTGGGTTACGCTGGTCTACGA
5520
Gln Gln His Gly Leu Ala Val Leu His Glu
lacI
5530 5540 5550
CCACGCCCAGTCGCGTACCATCTTCATGGG
GGTGCGGGTCAGCGCATGGTAGAAGTACCC
5550
Val Gly Leu Arg Thr Gly Asp Glu His Ser
lacI
5560 5570 5580
AGAAAATAATACTGTTGATGGGTGTCTGGT
TCTTTTATTATGACAACTACCCACAGACCA
5580
Phe Ile Ile Ser Asn Ile Pro Thr Gln Asp
lacI
5590 5600 5610
CAGAGACATCAAGAAATAACGCCGGAACAT
GTCTCTGTAGTTCTTTATTGCGGCCTTGTA
5610
Ser Val Asp Leu Phe Leu Ala Pro Val Asn
lacI
5620 5630 5640
TAGTGCAGGCAGCTTCCACAGCAATGGCAT
ATCACGTCCGTCGAAGGTGTCGTTACCGTA
5640
Thr Cys Ala Ala Glu Val Ala Ile Ala Asp
lacI
5650 5660 5670
CCTGGTCATCCAGCGGATAGTTAATGATCA
GGACCAGTAGGTCGCCTATCAATTACTAGT
5670
Gln Asp Asp Leu Pro Tyr Asn Ile Ile Leu
lacI
5680 5690 5700
GCCCACTGACGCGTTGCGCGAGAAGATTGT
CGGGTGACTGCGCAACGCGCTCTTCTAACA
5700
Gly Ser Val Arg Gln Ala Leu Leu Asn His
lacI
5710 5720 5730
GCACCGCCGCTTTACAGGCTTCGACGCCGC
CGTGGCGGCGAAATGTCCGAAGCTGCGGCG
5730
Val Ala Ala Lys Cys Ala Glu Val Gly Ser
lacI
5740 5750 5760
TTCGTTCTACCATCGACACCACCACGCTGG
AAGCAAGATGGTAGCTGTGGTGGTGCGACC
5760
Arg Glu Val Met Ser Val Val Val Ser Ala
lacI
5770 5780 5790
CACCCAGTTGATCGGCGCGAGATTTAATCG
GTGGGTCAACTAGCCGCGCTCTAAATTAGC
5790
Gly Leu Gln Asp Ala Arg Ser Lys Ile Ala
lacI
5800 5810 5820
CCGCGACAATTTGCGACGGCGCGTGCAGGG
GGCGCTGTTAAACGCTGCCGCGCACGTCCC
5820
Ala Val Ile Gln Ser Pro Ala His Leu Ala
lacI
5830 5840 5850
CCAGACTGGAGGTGGCAACGCCAATCAGCA
GGTCTGACCTCCACCGTTGCGGTTAGTCGT
5850
Leu Ser Ser Thr Ala Val Gly Ile Leu Leu
lacI
5860 5870 5880
ACGACTGTTTGCCCGCCAGTTGTTGTGCCA
TGCTGACAAACGGGCGGTCAACAACACGGT
5880
Ser Gln Lys Gly Ala Leu Gln Gln Ala Val
lacI
5890 5900 5910
CGCGGTTGGGAATGTAATTCAGCTCCGCCA
GCGCCAACCCTTACATTAAGTCGAGGCGGT
5910
Arg Asn Pro Ile Tyr Asn Leu Glu Ala Met
lacI
5920 5930 5940
TCGCCGCTTCCACTTTTTCCCGCGTTTTCG
AGCGGCGAAGGTGAAAAAGGGCGCAAAAGC
5940
Ala Ala Glu Val Lys Glu Arg Thr Lys Ala
lacI
5950 5960 5970
CAGAAACGTGGCTGGCCTGGTTCACCACGC
GTCTTTGCACCGACCGGACCAAGTGGTGCG
5970
Ser Val His Ser Ala Gln Asn Val Val Arg
lacI
5980 5990 6000
GGGAAACGGTCTGATAAGAGACACCGGCAT
CCCTTTGCCAGACTATTCTCTGTGGCCGTA
6000
Ser Val Thr Gln Tyr Ser Val Gly Ala Tyr
lacI
6010 6020 6030
ACTCTGCGACATCGTATAACGTTACTGGTT
TGAGACGCTGTAGCATATTGCAATGACCAA
6030
Glu Ala Val Asp Tyr Leu Thr Val Pro Lys
lacI
6040 6050 6060
TCACATTCACCACCCTGAATTGACTCTCTT
AGTGTAAGTGGTGGGACTTAACTGAGAGAA
6060
Val
lacI
lacI promoter
6070 6080 6090
CCGGGCGCTATCATGCCATACCGCGAAAGG
GGCCCGCGATAGTACGGTATGGCGCTTTCC
6090
lacI promoter
6100 6110 6120
TTTTGCGCCATTCGATGGTGTCCGGGATCT
AAAACGCGGTAAGCTACCACAGGCCCTAGA
6120
lacI promoter
6130 6140 6150
CGACGCTCTCCCTTATGCGACTCCTGCATT
GCTGCGAGAGGGAATACGCTGAGGACGTAA
6150
6160
AGGAAGCAGCC
TCCTTCGTCGG
6161