Features
Cut sites
CDS
misc_feature
promoter
protein_bind
RBS
rep_origin
terminator
Circle
Line
Sequence
Zoom
Reset
1000
2000
3000
4000
5000
6000
T7 promoter
lac operator
RBS
PmeI
N entry junction
N_JUN
DBD entry junction
DBD_JUN
C entry junction
C_JUN
C internal stuffer
C_JUN barcode
cloning scar
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
BsaI
(370)
BbsI
(1367)
BbsI
(1397)
BsaI
(1446)
AP-1 DESynR round 3
6435 bp
1000
2000
3000
4000
5000
6000
N entry junction
N_JUN
DBD_JUN barcode
N_JUN barcode
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
RBS
PmeI
DBD entry junction
DBD_JUN
C entry junction
C_JUN
C internal stuffer
C_JUN barcode
cloning scar
cloning scar
cloning scar
6xHis
PmeI
T7 terminator
BsaI
(370)
BbsI
(1367)
BbsI
(1397)
BsaI
(1446)
AP-1 DESynR round 3
6435 bp
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
5500
6000
N_JUN
DBD_JUN
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
RBS
PmeI
N entry junction
DBD entry junction
C entry junction
C_JUN
C internal stuffer
C_JUN barcode
cloning scar
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
PmeI
T7 terminator
BsaI
(370)
BbsI
(1367)
BbsI
(1397)
BsaI
(1446)
AP-1 DESynR round 3
6435 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
3400
3600
3800
4000
4200
4400
4600
4800
5000
5200
5400
5600
5800
6000
6200
6400
RBS
N_JUN
DBD entry junction
DBD_JUN
C_JUN
PmeI
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
T7 promoter
lac operator
PmeI
N entry junction
C entry junction
C internal stuffer
C_JUN barcode
cloning scar
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
T7 terminator
BsaI
(370)
BbsI
(1367)
BbsI
(1397)
BsaI
(1446)
AP-1 DESynR round 3
6435 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
3400
3500
3600
3700
3800
3900
4000
4100
4200
4300
4400
4500
4600
4700
4800
4900
5000
5100
5200
5300
5400
5500
5600
5700
5800
5900
6000
6100
6200
6300
6400
T7 promoter
lac operator
RBS
N_JUN
DBD entry junction
DBD_JUN
C_JUN
T7 terminator
f1 ori
KanR
KanR promoter
ori
bom
rop
lacI
lacI promoter
PmeI
N entry junction
C entry junction
C internal stuffer
C_JUN barcode
cloning scar
DBD_JUN barcode
cloning scar
N_JUN barcode
cloning scar
6xHis
PmeI
BsaI
(370)
BbsI
(1367)
BbsI
(1397)
BsaI
(1446)
AP-1 DESynR round 3
6435 bp
Both strands
Copy
10 20 30 40 50 60
CAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGAT
GTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90 100 110 120
GGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCT
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150 160 170 180
CATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCC
GTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210 220 230 240
AGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGA
TCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270 280 290 300
GATCGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAA
CTAGCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330 340 350 360
CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAGTTTAAACTA
GTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGTCAAATTTGAT
360
lac operator
RBS
PmeI
370 380 390 400 410 420
TGGGGTCTCAAGGAATGACCGCCAAGATGGAAACCACCTTCTACGACGACGCCCTGAACG
ACCCCAGAGTTCCTTACTGGCGGTTCTACCTTTGGTGGAAGATGCTGCTGCGGGACTTGC
420
N entry junction
Met Thr Ala Lys Met Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn
N_JUN
BsaI
430 440 450 460 470 480
CCAGCTTCCTGCCCAGCGAGAGCGGCCCCTACGGCTACAGCAACCCCAAGATCCTGAAGC
GGTCGAAGGACGGGTCGCTCTCGCCGGGGATGCCGATGTCGTTGGGGTTCTAGGACTTCG
480
Ala Ser Phe Leu Pro Ser Glu Ser Gly Pro Tyr Gly Tyr Ser Asn Pro Lys Ile Leu Lys
N_JUN
490 500 510 520 530 540
AGAGCATGACCCTGAACCTGGCCGACCCCGTGGGCAGCCTGAAGCCCCACCTGAGAGCCA
TCTCGTACTGGGACTTGGACCGGCTGGGGCACCCGTCGGACTTCGGGGTGGACTCTCGGT
540
Gln Ser Met Thr Leu Asn Leu Ala Asp Pro Val Gly Ser Leu Lys Pro His Leu Arg Ala
N_JUN
550 560 570 580 590 600
AGAACAGCGACCTGCTGACCAGCCCCGACGTGGGCCTGCTGAAGCTGGCCAGCCCCGAGC
TCTTGTCGCTGGACGACTGGTCGGGGCTGCACCCGGACGACTTCGACCGGTCGGGGCTCG
600
Lys Asn Ser Asp Leu Leu Thr Ser Pro Asp Val Gly Leu Leu Lys Leu Ala Ser Pro Glu
N_JUN
610 620 630 640 650 660
TGGAGAGACTGATCATCCAGAGCAGCAACGGCCACATCACCACCACCCCCACCCCCACCC
ACCTCTCTGACTAGTAGGTCTCGTCGTTGCCGGTGTAGTGGTGGTGGGGGTGGGGGTGGG
660
Leu Glu Arg Leu Ile Ile Gln Ser Ser Asn Gly His Ile Thr Thr Thr Pro Thr Pro Thr
N_JUN
670 680 690 700 710 720
AGTTCCTGTGCCCCAAGAACGTGACCGACGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGA
TCAAGGACACGGGGTTCTTGCACTGGCTGCTCGTCCTCCCGAAGCGGCTCCCGAAGCACT
720
Gln Phe Leu Cys Pro Lys Asn Val Thr Asp Glu Gln Glu Gly Phe Ala Glu Gly Phe Val
N_JUN
730 740 750 760 770 780
GAGCCCTGGCCGAGCTGCACAGCCAGAACACCCTGCCCAGCGTGACCAGCGCCGCCCAGC
CTCGGGACCGGCTCGACGTGTCGGTCTTGTGGGACGGGTCGCACTGGTCGCGGCGGGTCG
780
Arg Ala Leu Ala Glu Leu His Ser Gln Asn Thr Leu Pro Ser Val Thr Ser Ala Ala Gln
N_JUN
790 800 810 820 830 840
CCGTGAACGGCGCCGGCATGGTGGCCCCCGCCGTGGCCAGCGTGGCCGGCGGCAGCGGCA
GGCACTTGCCGCGGCCGTACCACCGGGGGCGGCACCGGTCGCACCGGCCGCCGTCGCCGT
840
Pro Val Asn Gly Ala Gly Met Val Ala Pro Ala Val Ala Ser Val Ala Gly Gly Ser Gly
N_JUN
850 860 870 880 890 900
GCGGCGGCTTCAGCGCCAGCCTGCACAGCGAGCCCCCCGTGTACGCCAACCTGAGCAACT
CGCCGCCGAAGTCGCGGTCGGACGTGTCGCTCGGGGGGCACATGCGGTTGGACTCGTTGA
900
Ser Gly Gly Phe Ser Ala Ser Leu His Ser Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn
N_JUN
910 920 930 940 950 960
TCAACCCCGGCGCCCTGAGCAGCGGCGGCGGCGCCCCCAGCTACGGCGCCGCCGGCCTGG
AGTTGGGGCCGCGGGACTCGTCGCCGCCGCCGCGGGGGTCGATGCCGCGGCGGCCGGACC
960
Phe Asn Pro Gly Ala Leu Ser Ser Gly Gly Gly Ala Pro Ser Tyr Gly Ala Ala Gly Leu
N_JUN
970 980 990 1000 1010 1020
CCTTCCCCGCCCAGCCCCAGCAGCAGCAGCAGCCCCCCCACCATCTGCCCCAGCAGATGC
GGAAGGGGCGGGTCGGGGTCGTCGTCGTCGTCGGGGGGGTGGTAGACGGGGTCGTCTACG
1020
Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln Gln Pro Pro His His Leu Pro Gln Gln Met
N_JUN
1030 1040 1050 1060 1070 1080
CCGTGCAGCACCCCAGACTGCAGGCCCTGAAGGAGGAGCCCCAGACCGTGCCCGAGATGC
GGCACGTCGTGGGGTCTGACGTCCGGGACTTCCTCCTCGGGGTCTGGCACGGGCTCTACG
1080
Pro Val Gln His Pro Arg Leu Gln Ala Leu Lys Glu Glu Pro Gln Thr Val Pro Glu Met
N_JUN
1090 1100 1110 1120 1130 1140
CCGGCGAAACCCCCCCCCTGAGCCCCATCGACATGGAGAGCCAAGATAGAATCAAGGCCG
GGCCGCTTTGGGGGGGGGACTCGGGGTAGCTGTACCTCTCGGTTCTATCTTAGTTCCGGC
1140
Pro Gly Glu Thr Pro Pro Leu Ser Pro Ile Asp Met Glu Ser
N_JUN
DBD entry junction
Arg Ile Lys Ala
DBD_JUN
1150 1160 1170 1180 1190 1200
AGAGAAAGAGAATGAGAAACAGAATCGCCGCCAGCAAGTGCAGAAAGAGAAAGCTGGAGA
TCTCTTTCTCTTACTCTTTGTCTTAGCGGCGGTCGTTCACGTCTTTCTCTTTCGACCTCT
1200
Glu Arg Lys Arg Met Arg Asn Arg Ile Ala Ala Ser Lys Cys Arg Lys Arg Lys Leu Glu
DBD_JUN
1210 1220 1230 1240 1250 1260
GAATCGCCAGACTGGAGGAGAAGGTGAAAACCCTGAAGGCCCAGAACAGCGAGCTGGCCA
CTTAGCGGTCTGACCTCCTCTTCCACTTTTGGGACTTCCGGGTCTTGTCGCTCGACCGGT
1260
Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala
DBD_JUN
1270 1280 1290 1300 1310 1320
GCACCGCCAACATGCTGAGAGAACAGGTGGCCCAGCTGAAGCAGAAGGTGATGAAGCATG
CGTGGCGGTTGTACGACTCTCTTGTCCACCGGGTCGACTTCGTCTTCCACTACTTCGTAC
1320
Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Val Met
DBD_JUN
C entry junction
C_JUN
1330 1340 1350 1360 1370 1380
TGAACAGCGGCTGCCAGCTGATGCTGACCCAGCAGCTGCAGACCTTCAGGAAAGTCTTCA
ACTTGTCGCCGACGGTCGACTACGACTGGGTCGTCGACGTCTGGAAGTCCTTTCAGAAGT
1380
Val Asn Ser Gly Cys Gln Leu Met Leu Thr Gln Gln Leu Gln Thr Phe
C_JUN
C internal stuffer
BbsI
1390 1400 1410 1420 1430 1440
GCCCGGGCAGAAGACAATTCCACTATTCACATTTCCCACATAGTCGATTCCTACCATTGT
CGGGCCCGTCTTCTGTTAAGGTGATAAGTGTAAAGGGTGTATCAGCTAAGGATGGTAACA
1440
C internal stuffer
C_JUN barcode
cloning scar
DBD_JUN barcode
cloning scar
N_JUN barcode
BbsI
1450 1460 1470 1480 1490 1500
CTTTCCTTCCGGAGACCACCACCACCACCACCACTGGTAGTTTAAACATGAGATCCGGCT
GAAAGGAAGGCCTCTGGTGGTGGTGGTGGTGGTGACCATCAAATTTGTACTCTAGGCCGA
1500
N_JUN barcode
cloning scar
His His His His His His
6xHis
PmeI
BsaI
1510 1520 1530 1540 1550 1560
GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCA
CGATTGTTTCGGGCTTTCCTTCGACTCAACCGACGACGGTGGCGACTCGTTATTGATCGT
1560
T7 terminator
1570 1580 1590 1600 1610 1620
TAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATA
ATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATAT
1620
T7 terminator
1630 1640 1650 1660 1670 1680
TCCGGATTGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGG
AGGCCTAACCGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACC
1680
f1 ori
1690 1700 1710 1720 1730 1740
TTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCT
AATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGA
1740
f1 ori
1750 1760 1770 1780 1790 1800
TCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCC
AGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGG
1800
f1 ori
1810 1820 1830 1840 1850 1860
CTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTG
GAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCAC
1860
f1 ori
1870 1880 1890 1900 1910 1920
ATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGT
TACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCA
1920
f1 ori
1930 1940 1950 1960 1970 1980
CCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGG
GGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCC
1980
f1 ori
1990 2000 2010 2020 2030 2040
TCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGC
AGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCG
2040
f1 ori
2050 2060 2070 2080 2090 2100
TGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTG
ACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGTCCAC
2100
f1 ori
2110 2120 2130 2140 2150 2160
GCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAA
CGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTT
2160
2170 2180 2190 2200 2210 2220
ATATGTATCCGCTCATGAATTAATTCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCG
TATACATAGGCGAGTACTTAATTAAGAGTCTTCTTGAGCAGTTCTTCCGCTATCTTCCGC
2220
Phe Phe Glu Asp Leu Leu Arg Tyr Phe Ala
Ter
KanR
2230 2240 2250 2260 2270 2280
ATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCG
TACGCGACGCTTAGCCCTCGCCGCTATGGCATTTCGTGCTCCTTCGCCAGTCGGGTAAGC
2280
Ile Arg Gln Ser Asp Pro Ala Ala Ile Gly Tyr Leu Val Leu Phe Arg Asp Ala Trp Glu
KanR
2290 2300 2310 2320 2330 2340
CCGCCAAGCTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCC
GGCGGTTCGAGAAGTCGTTATAGTGCCCATCGGTTGCGATACAGGACTATCGCCAGGCGG
2340
Gly Gly Leu Glu Glu Ala Ile Asp Arg Thr Ala Leu Ala Ile Asp Gln Tyr Arg Asp Ala
KanR
2350 2360 2370 2380 2390 2400
ACACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTC
TGTGGGTCGGCCGGTGTCAGCTACTTAGGTCTTTTCGCCGGTAAAAGGTGGTACTATAAG
2400
Val Gly Leu Arg Gly Cys Asp Ile Phe Gly Ser Phe Arg Gly Asn Glu Val Met Ile Asn
KanR
2410 2420 2430 2440 2450 2460
GGCAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATGCTCGCCTTG
CCGTTCGTCCGTAGCGGTACCCAGTGCTGCTCTAGGAGCGGCAGCCCGTACGAGCGGAAC
2460
Pro Leu Cys Ala Asp Gly His Thr Val Val Leu Asp Glu Gly Asp Pro Met Ser Ala Lys
KanR
2470 2480 2490 2500 2510 2520
AGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGA
TCGGACCGCTTGTCAAGCCGACCGCGCTCGGGGACTACGAGAAGCAGGTCTAGTAGGACT
2520
Leu Arg Ala Phe Leu Glu Ala Pro Ala Leu Gly Gln His Glu Glu Asp Leu Asp Asp Gln
KanR
2530 2540 2550 2560 2570 2580
TCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGG
AGCTGTTCTGGCCGAAGGTAGGCTCATGCACGAGCGAGCTACGCTACAAAGCGAACCACC
2580
Asp Val Leu Gly Ala Glu Met Arg Thr Arg Ala Arg Glu Ile Arg His Lys Ala Gln His
KanR
2590 2600 2610 2620 2630 2640
TCGAATGGGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATG
AGCTTACCCGTCCATCGGCCTAGTTCGCATACGTCGGCGGCGTAACGTAGTCGGTACTAC
2640
Asp Phe Pro Cys Thr Ala Pro Asp Leu Thr His Leu Arg Arg Met Ala Asp Ala Met Ile
KanR
2650 2660 2670 2680 2690 2700
GATACTTTCTCGGCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCC
CTATGAAAGAGCCGTCCTCGTTCCACTCTACTGTCCTCTAGGACGGGGCCGTGAAGCGGG
2700
Ser Val Lys Glu Ala Pro Ala Leu His Ser Ser Leu Leu Asp Gln Gly Pro Val Glu Gly
KanR
2710 2720 2730 2740 2750 2760
AATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACG
TTATCGTCGGTCAGGGAAGGGCGAAGTCACTGTTGCAGCTCGTGTCGACGCGTTCCTTGC
2760
Leu Leu Leu Trp Asp Arg Gly Ala Glu Thr Val Val Asp Leu Val Ala Ala Cys Pro Val
KanR
2770 2780 2790 2800 2810 2820
CCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCTTGCAGTTCATTCAGGGCACCG
GGGCAGCACCGGTCGGTGCTATCGGCGCGACGGAGCAGAACGTCAAGTAAGTCCCGTGGC
2820
Gly Thr Thr Ala Leu Trp Ser Leu Arg Ala Ala Glu Asp Gln Leu Glu Asn Leu Ala Gly
KanR
2830 2840 2850 2860 2870 2880
GACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCG
CTGTCCAGCCAGAACTGTTTTTCTTGGCCCGCGGGGACGCGACTGTCGGCCTTGTGCCGC
2880
Ser Leu Asp Thr Lys Val Phe Leu Val Pro Arg Gly Gln Ala Ser Leu Arg Phe Val Ala
KanR
2890 2900 2910 2920 2930 2940
GCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAA
CGTAGTCTCGTCGGCTAACAGACAACACGGGTCAGTATCGGCTTATCGGAGAGGTGGGTT
2940
Ala Asp Ser Cys Gly Ile Thr Gln Gln Ala Trp Asp Tyr Gly Phe Leu Arg Glu Val Trp
KanR
2950 2960 2970 2980 2990 3000
GCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCATACTCTTCCTTTTTCAATAT
CGCCGGCCTCTTGGACGCACGTTAGGTAGAACAAGTTAGTATGAGAAGGAAAAAGTTATA
3000
Ala Ala Pro Ser Gly Ala His Leu Gly Asp Gln Glu Ile Met
KanR
KanR promoter
3010 3020 3030 3040 3050 3060
TATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAG
ATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATC
3060
KanR promoter
3070 3080 3090 3100 3110 3120
AAAAATAAACAAATAGGGGTTCCGCGAACACCCCTTGTATTACTGTTTATGTAAGCAGAC
TTTTTATTTGTTTATCCCCAAGGCGCTTGTGGGGAACATAATGACAAATACATTCGTCTG
3120
KanR promoter
3130 3140 3150 3160 3170 3180
AGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGA
TCAAAATAACAAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCT
3180
3190 3200 3210 3220 3230 3240
CCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTG
GGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGAC
3240
ori
3250 3260 3270 3280 3290 3300
CTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACC
GAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGG
3300
ori
3310 3320 3330 3340 3350 3360
AACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCT
TTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGA
3360
ori
3370 3380 3390 3400 3410 3420
AGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGC
TCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCG
3420
ori
3430 3440 3450 3460 3470 3480
TCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTT
AGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAA
3480
ori
3490 3500 3510 3520 3530 3540
GGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTG
CCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCAC
3540
ori
3550 3560 3570 3580 3590 3600
CACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCT
GTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGA
3600
ori
3610 3620 3630 3640 3650 3660
ATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAG
TACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTC
3660
ori
3670 3680 3690 3700 3710 3720
GGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAG
CCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATC
3720
ori
3730 3740 3750 3760 3770 3780
TCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGG
AGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCC
3780
ori
3790 3800 3810 3820 3830 3840
GCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTG
CGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGAC
3840
ori
3850 3860 3870 3880 3890 3900
GCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTAC
CGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATG
3900
3910 3920 3930 3940 3950 3960
CGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT
GCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCA
3960
3970 3980 3990 4000 4010 4020
GAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAT
CTCGCTCCTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATA
4020
bom
4030 4040 4050 4060 4070 4080
TTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGC
AAGTGTGGCGTATATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCG
4080
bom
4090 4100 4110 4120 4130 4140
CAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAA
GTCATATGTGAGGCGATAGCGATGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTT
4140
bom
4150 4160 4170 4180 4190 4200
CACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTG
GTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGAC
4200
4210 4220 4230 4240 4250 4260
TGACCGTCACCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGA
ACTGGCAGTGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT
4260
Leu Asn Glu Gly Asp Asp Gly Phe Arg Ala Leu
Ter
rop
4270 4280 4290 4300 4310 4320
GGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTT
CCGTCGACGCCATTTCGAGTAGTCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAA
4320
Cys Ser Arg Tyr Leu Glu Asp Ala His Asp His Leu Ser Glu Cys Ile Asp Ala Gln Glu
rop
4330 4340 4350 4360 4370 4380
CATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGC
GTAGGCGCAGGTCGAGCAACTCAAAGAGGTCTTCGCAATTACAGACCGAAGACTATTTCG
4380
Asp Ala Asp Leu Glu Asn Leu Lys Glu Leu Leu Thr Leu Thr Gln Ser Arg Ile Phe Arg
rop
4390 4400 4410 4420 4430 4440
GGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGAT
CCCGGTACAATTCCCGCCAAAAAAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTA
4440
Ala Met Asn Leu Ala Thr Lys Glu Gln Lys Thr Val
rop
4450 4460 4470 4480 4490 4500
TTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTA
AAGACAAGTACCCCCATTACTATGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAAT
4500
4510 4520 4530 4540 4550 4560
CTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGA
GACTACTACTTGTACGGGCCAATGACCTTGCAACACTCCCATTTGTTGACCGCCATACCT
4560
4570 4580 4590 4600 4610 4620
TGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATG
ACGCCGCCCTGGTCTCTTTTTAGTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTAC
4620
4630 4640 4650 4660 4670 4680
TAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGC
ATCCACAAGGTGTCCCATCGGTCGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACG
4680
4690 4700 4710 4720 4730 4740
AGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCAAAGACCATTCATG
TCCCGCGACTGAAGGCGCAAAGGTCTGAAATGCTTTGTGCCTTTGGTTTCTGGTAAGTAC
4740
4750 4760 4770 4780 4790 4800
TTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCG
AACAACGAGTCCAGCGTCTGCAAAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGC
4800
4810 4820 4830 4840 4850 4860
GTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACA
CACTAAGTAAGACGATTGGTCATTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGT
4860
4870 4880 4890 4900 4910 4920
GGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGC
CCTCGTGCTAGTACGCGTGGGCACCCCGGCGGTACGGCCGCTATTACCGGACGAAGAGCG
4920
4930 4940 4950 4960 4970 4980
CGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGA
GCTTTGCAAACCACCGCCCTGGTCACTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCT
4980
4990 5000 5010 5020 5030 5040
ATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAA
TATGGCGTTCGCTGTCCGGCTAGTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCTTTT
5040
5050 5060 5070 5080 5090 5100
TGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAAAAGACAGTCATAA
ACTGGGTCTCGCGACGGCCGTGGACAGGATGCTCAACGTACTATTTTTTCTGTCAGTATT
5100
5110 5120 5130 5140 5150 5160
GTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTC
CACGCCGCTGCTATCAGTACGGGGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAG
5160
5170 5180 5190 5200 5210 5220
TCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGT
AGTTCCCGTAGCCAGCTCTAGGGCCACGGATTACTCACTCGATTGAATGTAATTAACGCA
5220
5230 5240 5250 5260 5270 5280
TGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG
ACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGC
5280
Gln Gly Ser Glu Leu Arg Ser Val Gln Arg Ala Leu Gln Met Leu Ser Asp
Ter
lacI
5290 5300 5310 5320 5330 5340
GCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACC
CGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGG
5340
Ala Leu Ala Arg Pro Ser Ala Thr Gln Thr Asn Pro Ala Leu Thr Thr Lys Arg Lys Val
lacI
5350 5360 5370 5380 5390 5400
AGTGAAACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAG
TCACTTTGCCCGTTGTCGACTAACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTC
5400
Leu Ser Val Pro Leu Leu Gln Asn Gly Lys Val Ala Gln Gly Gln Ser Leu Gln Leu Leu
lacI
5410 5420 5430 5440 5450 5460
CGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGG
GCCAGGTGCGACCAAACGGGGTCGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCC
5460
Arg Asp Val Ser Thr Gln Gly Leu Leu Arg Phe Asp Gln Lys Ile Thr Thr Leu Pro Pro
lacI
5470 5480 5490 5500 5510 5520
ATATAACATGAGCTATCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACG
TATATTGTACTCGATAGAAGCCATAGCAGCATAGGGTGATGGCTCTACAGGCGTGGTTGC
5520
Ile Tyr Cys Ser Ser Asp Glu Thr Asp Asp Tyr Gly Val Val Ser Ile Asp Ala Gly Val
lacI
5530 5540 5550 5560 5570 5580
CGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACC
GCGTCGGGCCTGAGCCATTACCGCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGG
5580
Arg Leu Gly Ser Glu Thr Ile Ala Arg Met Ala Gly Leu Ala Met Gln Asp Asn Ala Val
lacI
5590 5600 5610 5620 5630 5640
AGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGAC
TCGTAGCGTCACCCTTGCTACGGGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTG
5640
Leu Met Ala Thr Pro Val Ile Gly Glu Asn Leu Met Gln Met Thr Gln Gln Phe Gly Ser
lacI
5650 5660 5670 5680 5690 5700
ATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATAT
TACCGTGAGGTCAGCGGAAGGGCAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATA
5700
Met Ala Ser Trp Asp Gly Glu Arg Glu Ala Ile Pro Gln Ile Gln Asn Arg Thr Leu Tyr
lacI
5710 5720 5730 5740 5750 5760
TTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGC
AATACGGTCGGTCGGTCTGCGTCTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCG
5760
Lys His Trp Gly Ala Leu Arg Leu Arg Ala Ser Val Ser Ser Leu Pro Gly Ala Leu Leu
lacI
5770 5780 5790 5800 5810 5820
GCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCATCTTCA
CGCTAAACGACCACTGGGTTACGCTGGTCTACGAGGTGCGGGTCAGCGCATGGTAGAAGT
5820
Ala Ile Gln Gln His Gly Leu Ala Val Leu His Glu Val Gly Leu Arg Thr Gly Asp Glu
lacI
5830 5840 5850 5860 5870 5880
TGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGA
ACCCTCTTTTATTATGACAACTACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCT
5880
His Ser Phe Ile Ile Ser Asn Ile Pro Thr Gln Asp Ser Val Asp Leu Phe Leu Ala Pro
lacI
5890 5900 5910 5920 5930 5940
ACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATG
TGTAATCACGTCCGTCGAAGGTGTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTAC
5940
Val Asn Thr Cys Ala Ala Glu Val Ala Ile Ala Asp Gln Asp Asp Leu Pro Tyr Asn Ile
lacI
5950 5960 5970 5980 5990 6000
ATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACG
TAGTCGGGTGACTGCGCAACGCGCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGC
6000
Ile Leu Gly Ser Val Arg Gln Ala Leu Leu Asn His Val Ala Ala Lys Cys Ala Glu Val
lacI
6010 6020 6030 6040 6050 6060
CCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTA
GGCGAAGCAAGATGGTAGCTGTGGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAAT
6060
Gly Ser Arg Glu Val Met Ser Val Val Val Ser Ala Gly Leu Gln Asp Ala Arg Ser Lys
lacI
6070 6080 6090 6100 6110 6120
ATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATC
TAGCGGCGCTGTTAAACGCTGCCGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAG
6120
Ile Ala Ala Val Ile Gln Ser Pro Ala His Leu Ala Leu Ser Ser Thr Ala Val Gly Ile
lacI
6130 6140 6150 6160 6170 6180
AGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCC
TCGTTGCTGACAAACGGGCGGTCAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGG
6180
Leu Leu Ser Gln Lys Gly Ala Leu Gln Gln Ala Val Arg Asn Pro Ile Tyr Asn Leu Glu
lacI
6190 6200 6210 6220 6230 6240
GCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACC
CGGTAGCGGCGAAGGTGAAAAAGGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGG
6240
Ala Met Ala Ala Glu Val Lys Glu Arg Thr Lys Ala Ser Val His Ser Ala Gln Asn Val
lacI
6250 6260 6270 6280 6290 6300
ACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACT
TGCGCCCTTTGCCAGACTATTCTCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGA
6300
Val Arg Ser Val Thr Gln Tyr Ser Val Gly Ala Tyr Glu Ala Val Asp Tyr Leu Thr Val
lacI
6310 6320 6330 6340 6350 6360
GGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGA
CCAAAGTGTAAGTGGTGGGACTTAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCT
6360
Pro Lys Val
lacI
lacI promoter
6370 6380 6390 6400 6410 6420
AAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTG
TTCCAAAACGCGGTAAGCTACCACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGAC
6420
lacI promoter
6430
CATTAGGAAGCAGCC
GTAATCCTTCGTCGG
6435
10 20 30
CAGTAGTAGGTTGAGGCCGTTGAGCACCGC
GTCATCATCCAACTCCGGCAACTCGTGGCG
30
40 50 60
CGCCGCAAGGAATGGTGCATGCAAGGAGAT
GCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90
GGCGCCCAACAGTCCCCCGGCCACGGGGCC
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGG
90
100 110 120
TGCCACCATACCCACGCCGAAACAAGCGCT
ACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150
CATGAGCCCGAAGTGGCGAGCCCGATCTTC
GTACTCGGGCTTCACCGCTCGGGCTAGAAG
150
160 170 180
CCCATCGGTGATGTCGGCGATATAGGCGCC
GGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210
AGCAACCGCACCTGTGGCGCCGGTGATGCC
TCGTTGGCGTGGACACCGCGGCCACTACGG
210
220 230 240
GGCCACGATGCGTCCGGCGTAGAGGATCGA
CCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270
GATCGATCTCGATCCCGCGAAATTAATACG
CTAGCTAGAGCTAGGGCGCTTTAATTATGC
270
T7 promoter
280 290 300
ACTCACTATAGGGGAATTGTGAGCGGATAA
TGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330
CAATTCCCCTCTAGAAATAATTTTGTTTAA
GTTAAGGGGAGATCTTTATTAAAACAAATT
330
lac operator
RBS
340 350 360
CTTTAAGAAGGAGATATACAGTTTAAACTA
GAAATTCTTCCTCTATATGTCAAATTTGAT
360
RBS
PmeI
370 380 390
TGGGGTCTCAAGGAATGACCGCCAAGATGG
ACCCCAGAGTTCCTTACTGGCGGTTCTACC
390
N entry junction
Met Thr Ala Lys Met
N_JUN
BsaI
400 410 420
AAACCACCTTCTACGACGACGCCCTGAACG
TTTGGTGGAAGATGCTGCTGCGGGACTTGC
420
Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn
N_JUN
430 440 450
CCAGCTTCCTGCCCAGCGAGAGCGGCCCCT
GGTCGAAGGACGGGTCGCTCTCGCCGGGGA
450
Ala Ser Phe Leu Pro Ser Glu Ser Gly Pro
N_JUN
460 470 480
ACGGCTACAGCAACCCCAAGATCCTGAAGC
TGCCGATGTCGTTGGGGTTCTAGGACTTCG
480
Tyr Gly Tyr Ser Asn Pro Lys Ile Leu Lys
N_JUN
490 500 510
AGAGCATGACCCTGAACCTGGCCGACCCCG
TCTCGTACTGGGACTTGGACCGGCTGGGGC
510
Gln Ser Met Thr Leu Asn Leu Ala Asp Pro
N_JUN
520 530 540
TGGGCAGCCTGAAGCCCCACCTGAGAGCCA
ACCCGTCGGACTTCGGGGTGGACTCTCGGT
540
Val Gly Ser Leu Lys Pro His Leu Arg Ala
N_JUN
550 560 570
AGAACAGCGACCTGCTGACCAGCCCCGACG
TCTTGTCGCTGGACGACTGGTCGGGGCTGC
570
Lys Asn Ser Asp Leu Leu Thr Ser Pro Asp
N_JUN
580 590 600
TGGGCCTGCTGAAGCTGGCCAGCCCCGAGC
ACCCGGACGACTTCGACCGGTCGGGGCTCG
600
Val Gly Leu Leu Lys Leu Ala Ser Pro Glu
N_JUN
610 620 630
TGGAGAGACTGATCATCCAGAGCAGCAACG
ACCTCTCTGACTAGTAGGTCTCGTCGTTGC
630
Leu Glu Arg Leu Ile Ile Gln Ser Ser Asn
N_JUN
640 650 660
GCCACATCACCACCACCCCCACCCCCACCC
CGGTGTAGTGGTGGTGGGGGTGGGGGTGGG
660
Gly His Ile Thr Thr Thr Pro Thr Pro Thr
N_JUN
670 680 690
AGTTCCTGTGCCCCAAGAACGTGACCGACG
TCAAGGACACGGGGTTCTTGCACTGGCTGC
690
Gln Phe Leu Cys Pro Lys Asn Val Thr Asp
N_JUN
700 710 720
AGCAGGAGGGCTTCGCCGAGGGCTTCGTGA
TCGTCCTCCCGAAGCGGCTCCCGAAGCACT
720
Glu Gln Glu Gly Phe Ala Glu Gly Phe Val
N_JUN
730 740 750
GAGCCCTGGCCGAGCTGCACAGCCAGAACA
CTCGGGACCGGCTCGACGTGTCGGTCTTGT
750
Arg Ala Leu Ala Glu Leu His Ser Gln Asn
N_JUN
760 770 780
CCCTGCCCAGCGTGACCAGCGCCGCCCAGC
GGGACGGGTCGCACTGGTCGCGGCGGGTCG
780
Thr Leu Pro Ser Val Thr Ser Ala Ala Gln
N_JUN
790 800 810
CCGTGAACGGCGCCGGCATGGTGGCCCCCG
GGCACTTGCCGCGGCCGTACCACCGGGGGC
810
Pro Val Asn Gly Ala Gly Met Val Ala Pro
N_JUN
820 830 840
CCGTGGCCAGCGTGGCCGGCGGCAGCGGCA
GGCACCGGTCGCACCGGCCGCCGTCGCCGT
840
Ala Val Ala Ser Val Ala Gly Gly Ser Gly
N_JUN
850 860 870
GCGGCGGCTTCAGCGCCAGCCTGCACAGCG
CGCCGCCGAAGTCGCGGTCGGACGTGTCGC
870
Ser Gly Gly Phe Ser Ala Ser Leu His Ser
N_JUN
880 890 900
AGCCCCCCGTGTACGCCAACCTGAGCAACT
TCGGGGGGCACATGCGGTTGGACTCGTTGA
900
Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn
N_JUN
910 920 930
TCAACCCCGGCGCCCTGAGCAGCGGCGGCG
AGTTGGGGCCGCGGGACTCGTCGCCGCCGC
930
Phe Asn Pro Gly Ala Leu Ser Ser Gly Gly
N_JUN
940 950 960
GCGCCCCCAGCTACGGCGCCGCCGGCCTGG
CGCGGGGGTCGATGCCGCGGCGGCCGGACC
960
Gly Ala Pro Ser Tyr Gly Ala Ala Gly Leu
N_JUN
970 980 990
CCTTCCCCGCCCAGCCCCAGCAGCAGCAGC
GGAAGGGGCGGGTCGGGGTCGTCGTCGTCG
990
Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln
N_JUN
1000 1010 1020
AGCCCCCCCACCATCTGCCCCAGCAGATGC
TCGGGGGGGTGGTAGACGGGGTCGTCTACG
1020
Gln Pro Pro His His Leu Pro Gln Gln Met
N_JUN
1030 1040 1050
CCGTGCAGCACCCCAGACTGCAGGCCCTGA
GGCACGTCGTGGGGTCTGACGTCCGGGACT
1050
Pro Val Gln His Pro Arg Leu Gln Ala Leu
N_JUN
1060 1070 1080
AGGAGGAGCCCCAGACCGTGCCCGAGATGC
TCCTCCTCGGGGTCTGGCACGGGCTCTACG
1080
Lys Glu Glu Pro Gln Thr Val Pro Glu Met
N_JUN
1090 1100 1110
CCGGCGAAACCCCCCCCCTGAGCCCCATCG
GGCCGCTTTGGGGGGGGGACTCGGGGTAGC
1110
Pro Gly Glu Thr Pro Pro Leu Ser Pro Ile
N_JUN
1120 1130 1140
ACATGGAGAGCCAAGATAGAATCAAGGCCG
TGTACCTCTCGGTTCTATCTTAGTTCCGGC
1140
Asp Met Glu Ser
N_JUN
DBD entry junction
Arg Ile Lys Ala
DBD_JUN
1150 1160 1170
AGAGAAAGAGAATGAGAAACAGAATCGCCG
TCTCTTTCTCTTACTCTTTGTCTTAGCGGC
1170
Glu Arg Lys Arg Met Arg Asn Arg Ile Ala
DBD_JUN
1180 1190 1200
CCAGCAAGTGCAGAAAGAGAAAGCTGGAGA
GGTCGTTCACGTCTTTCTCTTTCGACCTCT
1200
Ala Ser Lys Cys Arg Lys Arg Lys Leu Glu
DBD_JUN
1210 1220 1230
GAATCGCCAGACTGGAGGAGAAGGTGAAAA
CTTAGCGGTCTGACCTCCTCTTCCACTTTT
1230
Arg Ile Ala Arg Leu Glu Glu Lys Val Lys
DBD_JUN
1240 1250 1260
CCCTGAAGGCCCAGAACAGCGAGCTGGCCA
GGGACTTCCGGGTCTTGTCGCTCGACCGGT
1260
Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala
DBD_JUN
1270 1280 1290
GCACCGCCAACATGCTGAGAGAACAGGTGG
CGTGGCGGTTGTACGACTCTCTTGTCCACC
1290
Ser Thr Ala Asn Met Leu Arg Glu Gln Val
DBD_JUN
1300 1310 1320
CCCAGCTGAAGCAGAAGGTGATGAAGCATG
GGGTCGACTTCGTCTTCCACTACTTCGTAC
1320
Ala Gln Leu Lys Gln Lys Val Met
DBD_JUN
C entry junction
C_JUN
1330 1340 1350
TGAACAGCGGCTGCCAGCTGATGCTGACCC
ACTTGTCGCCGACGGTCGACTACGACTGGG
1350
Val Asn Ser Gly Cys Gln Leu Met Leu Thr
C_JUN
1360 1370 1380
AGCAGCTGCAGACCTTCAGGAAAGTCTTCA
TCGTCGACGTCTGGAAGTCCTTTCAGAAGT
1380
Gln Gln Leu Gln Thr Phe
C_JUN
C internal stuffer
BbsI
1390 1400 1410
GCCCGGGCAGAAGACAATTCCACTATTCAC
CGGGCCCGTCTTCTGTTAAGGTGATAAGTG
1410
C internal stuffer
C_JUN barcode
BbsI
1420 1430 1440
ATTTCCCACATAGTCGATTCCTACCATTGT
TAAAGGGTGTATCAGCTAAGGATGGTAACA
1440
cloning scar
DBD_JUN barcode
cloning scar
N_JUN barcode
C_JUN barcode
1450 1460 1470
CTTTCCTTCCGGAGACCACCACCACCACCA
GAAAGGAAGGCCTCTGGTGGTGGTGGTGGT
1470
N_JUN barcode
cloning scar
His His His His His
6xHis
BsaI
1480 1490 1500
CCACTGGTAGTTTAAACATGAGATCCGGCT
GGTGACCATCAAATTTGTACTCTAGGCCGA
1500
His
6xHis
PmeI
1510 1520 1530
GCTAACAAAGCCCGAAAGGAAGCTGAGTTG
CGATTGTTTCGGGCTTTCCTTCGACTCAAC
1530
1540 1550 1560
GCTGCTGCCACCGCTGAGCAATAACTAGCA
CGACGACGGTGGCGACTCGTTATTGATCGT
1560
T7 terminator
1570 1580 1590
TAACCCCTTGGGGCCTCTAAACGGGTCTTG
ATTGGGGAACCCCGGAGATTTGCCCAGAAC
1590
T7 terminator
1600 1610 1620
AGGGGTTTTTTGCTGAAAGGAGGAACTATA
TCCCCAAAAAACGACTTTCCTCCTTGATAT
1620
T7 terminator
1630 1640 1650
TCCGGATTGGCGAATGGGACGCGCCCTGTA
AGGCCTAACCGCTTACCCTGCGCGGGACAT
1650
f1 ori
1660 1670 1680
GCGGCGCATTAAGCGCGGCGGGTGTGGTGG
CGCCGCGTAATTCGCGCCGCCCACACCACC
1680
f1 ori
1690 1700 1710
TTACGCGCAGCGTGACCGCTACACTTGCCA
AATGCGCGTCGCACTGGCGATGTGAACGGT
1710
f1 ori
1720 1730 1740
GCGCCCTAGCGCCCGCTCCTTTCGCTTTCT
CGCGGGATCGCGGGCGAGGAAAGCGAAAGA
1740
f1 ori
1750 1760 1770
TCCCTTCCTTTCTCGCCACGTTCGCCGGCT
AGGGAAGGAAAGAGCGGTGCAAGCGGCCGA
1770
f1 ori
1780 1790 1800
TTCCCCGTCAAGCTCTAAATCGGGGGCTCC
AAGGGGCAGTTCGAGATTTAGCCCCCGAGG
1800
f1 ori
1810 1820 1830
CTTTAGGGTTCCGATTTAGTGCTTTACGGC
GAAATCCCAAGGCTAAATCACGAAATGCCG
1830
f1 ori
1840 1850 1860
ACCTCGACCCCAAAAAACTTGATTAGGGTG
TGGAGCTGGGGTTTTTTGAACTAATCCCAC
1860
f1 ori
1870 1880 1890
ATGGTTCACGTAGTGGGCCATCGCCCTGAT
TACCAAGTGCATCACCCGGTAGCGGGACTA
1890
f1 ori
1900 1910 1920
AGACGGTTTTTCGCCCTTTGACGTTGGAGT
TCTGCCAAAAAGCGGGAAACTGCAACCTCA
1920
f1 ori
1930 1940 1950
CCACGTTCTTTAATAGTGGACTCTTGTTCC
GGTGCAAGAAATTATCACCTGAGAACAAGG
1950
f1 ori
1960 1970 1980
AAACTGGAACAACACTCAACCCTATCTCGG
TTTGACCTTGTTGTGAGTTGGGATAGAGCC
1980
f1 ori
1990 2000 2010
TCTATTCTTTTGATTTATAAGGGATTTTGC
AGATAAGAAAACTAAATATTCCCTAAAACG
2010
f1 ori
2020 2030 2040
CGATTTCGGCCTATTGGTTAAAAAATGAGC
GCTAAAGCCGGATAACCAATTTTTTACTCG
2040
f1 ori
2050 2060 2070
TGATTTAACAAAAATTTAACGCGAATTTTA
ACTAAATTGTTTTTAAATTGCGCTTAAAAT
2070
f1 ori
2080 2090 2100
ACAAAATATTAACGTTTACAATTTCAGGTG
TGTTTTATAATTGCAAATGTTAAAGTCCAC
2100
f1 ori
2110 2120 2130
GCACTTTTCGGGGAAATGTGCGCGGAACCC
CGTGAAAAGCCCCTTTACACGCGCCTTGGG
2130
2140 2150 2160
CTATTTGTTTATTTTTCTAAATACATTCAA
GATAAACAAATAAAAAGATTTATGTAAGTT
2160
2170 2180 2190
ATATGTATCCGCTCATGAATTAATTCTCAG
TATACATAGGCGAGTACTTAATTAAGAGTC
2190
Ter
KanR
2200 2210 2220
AAGAACTCGTCAAGAAGGCGATAGAAGGCG
TTCTTGAGCAGTTCTTCCGCTATCTTCCGC
2220
Phe Phe Glu Asp Leu Leu Arg Tyr Phe Ala
KanR
2230 2240 2250
ATGCGCTGCGAATCGGGAGCGGCGATACCG
TACGCGACGCTTAGCCCTCGCCGCTATGGC
2250
Ile Arg Gln Ser Asp Pro Ala Ala Ile Gly
KanR
2260 2270 2280
TAAAGCACGAGGAAGCGGTCAGCCCATTCG
ATTTCGTGCTCCTTCGCCAGTCGGGTAAGC
2280
Tyr Leu Val Leu Phe Arg Asp Ala Trp Glu
KanR
2290 2300 2310
CCGCCAAGCTCTTCAGCAATATCACGGGTA
GGCGGTTCGAGAAGTCGTTATAGTGCCCAT
2310
Gly Gly Leu Glu Glu Ala Ile Asp Arg Thr
KanR
2320 2330 2340
GCCAACGCTATGTCCTGATAGCGGTCCGCC
CGGTTGCGATACAGGACTATCGCCAGGCGG
2340
Ala Leu Ala Ile Asp Gln Tyr Arg Asp Ala
KanR
2350 2360 2370
ACACCCAGCCGGCCACAGTCGATGAATCCA
TGTGGGTCGGCCGGTGTCAGCTACTTAGGT
2370
Val Gly Leu Arg Gly Cys Asp Ile Phe Gly
KanR
2380 2390 2400
GAAAAGCGGCCATTTTCCACCATGATATTC
CTTTTCGCCGGTAAAAGGTGGTACTATAAG
2400
Ser Phe Arg Gly Asn Glu Val Met Ile Asn
KanR
2410 2420 2430
GGCAAGCAGGCATCGCCATGGGTCACGACG
CCGTTCGTCCGTAGCGGTACCCAGTGCTGC
2430
Pro Leu Cys Ala Asp Gly His Thr Val Val
KanR
2440 2450 2460
AGATCCTCGCCGTCGGGCATGCTCGCCTTG
TCTAGGAGCGGCAGCCCGTACGAGCGGAAC
2460
Leu Asp Glu Gly Asp Pro Met Ser Ala Lys
KanR
2470 2480 2490
AGCCTGGCGAACAGTTCGGCTGGCGCGAGC
TCGGACCGCTTGTCAAGCCGACCGCGCTCG
2490
Leu Arg Ala Phe Leu Glu Ala Pro Ala Leu
KanR
2500 2510 2520
CCCTGATGCTCTTCGTCCAGATCATCCTGA
GGGACTACGAGAAGCAGGTCTAGTAGGACT
2520
Gly Gln His Glu Glu Asp Leu Asp Asp Gln
KanR
2530 2540 2550
TCGACAAGACCGGCTTCCATCCGAGTACGT
AGCTGTTCTGGCCGAAGGTAGGCTCATGCA
2550
Asp Val Leu Gly Ala Glu Met Arg Thr Arg
KanR
2560 2570 2580
GCTCGCTCGATGCGATGTTTCGCTTGGTGG
CGAGCGAGCTACGCTACAAAGCGAACCACC
2580
Ala Arg Glu Ile Arg His Lys Ala Gln His
KanR
2590 2600 2610
TCGAATGGGCAGGTAGCCGGATCAAGCGTA
AGCTTACCCGTCCATCGGCCTAGTTCGCAT
2610
Asp Phe Pro Cys Thr Ala Pro Asp Leu Thr
KanR
2620 2630 2640
TGCAGCCGCCGCATTGCATCAGCCATGATG
ACGTCGGCGGCGTAACGTAGTCGGTACTAC
2640
His Leu Arg Arg Met Ala Asp Ala Met Ile
KanR
2650 2660 2670
GATACTTTCTCGGCAGGAGCAAGGTGAGAT
CTATGAAAGAGCCGTCCTCGTTCCACTCTA
2670
Ser Val Lys Glu Ala Pro Ala Leu His Ser
KanR
2680 2690 2700
GACAGGAGATCCTGCCCCGGCACTTCGCCC
CTGTCCTCTAGGACGGGGCCGTGAAGCGGG
2700
Ser Leu Leu Asp Gln Gly Pro Val Glu Gly
KanR
2710 2720 2730
AATAGCAGCCAGTCCCTTCCCGCTTCAGTG
TTATCGTCGGTCAGGGAAGGGCGAAGTCAC
2730
Leu Leu Leu Trp Asp Arg Gly Ala Glu Thr
KanR
2740 2750 2760
ACAACGTCGAGCACAGCTGCGCAAGGAACG
TGTTGCAGCTCGTGTCGACGCGTTCCTTGC
2760
Val Val Asp Leu Val Ala Ala Cys Pro Val
KanR
2770 2780 2790
CCCGTCGTGGCCAGCCACGATAGCCGCGCT
GGGCAGCACCGGTCGGTGCTATCGGCGCGA
2790
Gly Thr Thr Ala Leu Trp Ser Leu Arg Ala
KanR
2800 2810 2820
GCCTCGTCTTGCAGTTCATTCAGGGCACCG
CGGAGCAGAACGTCAAGTAAGTCCCGTGGC
2820
Ala Glu Asp Gln Leu Glu Asn Leu Ala Gly
KanR
2830 2840 2850
GACAGGTCGGTCTTGACAAAAAGAACCGGG
CTGTCCAGCCAGAACTGTTTTTCTTGGCCC
2850
Ser Leu Asp Thr Lys Val Phe Leu Val Pro
KanR
2860 2870 2880
CGCCCCTGCGCTGACAGCCGGAACACGGCG
GCGGGGACGCGACTGTCGGCCTTGTGCCGC
2880
Arg Gly Gln Ala Ser Leu Arg Phe Val Ala
KanR
2890 2900 2910
GCATCAGAGCAGCCGATTGTCTGTTGTGCC
CGTAGTCTCGTCGGCTAACAGACAACACGG
2910
Ala Asp Ser Cys Gly Ile Thr Gln Gln Ala
KanR
2920 2930 2940
CAGTCATAGCCGAATAGCCTCTCCACCCAA
GTCAGTATCGGCTTATCGGAGAGGTGGGTT
2940
Trp Asp Tyr Gly Phe Leu Arg Glu Val Trp
KanR
2950 2960 2970
GCGGCCGGAGAACCTGCGTGCAATCCATCT
CGCCGGCCTCTTGGACGCACGTTAGGTAGA
2970
Ala Ala Pro Ser Gly Ala His Leu Gly Asp
KanR
2980 2990 3000
TGTTCAATCATACTCTTCCTTTTTCAATAT
ACAAGTTAGTATGAGAAGGAAAAAGTTATA
3000
Gln Glu Ile Met
KanR
KanR promoter
3010 3020 3030
TATTGAAGCATTTATCAGGGTTATTGTCTC
ATAACTTCGTAAATAGTCCCAATAACAGAG
3030
KanR promoter
3040 3050 3060
ATGAGCGGATACATATTTGAATGTATTTAG
TACTCGCCTATGTATAAACTTACATAAATC
3060
KanR promoter
3070 3080 3090
AAAAATAAACAAATAGGGGTTCCGCGAACA
TTTTTATTTGTTTATCCCCAAGGCGCTTGT
3090
KanR promoter
3100 3110 3120
CCCCTTGTATTACTGTTTATGTAAGCAGAC
GGGGAACATAATGACAAATACATTCGTCTG
3120
3130 3140 3150
AGTTTTATTGTTCATGACCAAAATCCCTTA
TCAAAATAACAAGTACTGGTTTTAGGGAAT
3150
3160 3170 3180
ACGTGAGTTTTCGTTCCACTGAGCGTCAGA
TGCACTCAAAAGCAAGGTGACTCGCAGTCT
3180
3190 3200 3210
CCCCGTAGAAAAGATCAAAGGATCTTCTTG
GGGGCATCTTTTCTAGTTTCCTAGAAGAAC
3210
ori
3220 3230 3240
AGATCCTTTTTTTCTGCGCGTAATCTGCTG
TCTAGGAAAAAAAGACGCGCATTAGACGAC
3240
ori
3250 3260 3270
CTTGCAAACAAAAAAACCACCGCTACCAGC
GAACGTTTGTTTTTTTGGTGGCGATGGTCG
3270
ori
3280 3290 3300
GGTGGTTTGTTTGCCGGATCAAGAGCTACC
CCACCAAACAAACGGCCTAGTTCTCGATGG
3300
ori
3310 3320 3330
AACTCTTTTTCCGAAGGTAACTGGCTTCAG
TTGAGAAAAAGGCTTCCATTGACCGAAGTC
3330
ori
3340 3350 3360
CAGAGCGCAGATACCAAATACTGTCCTTCT
GTCTCGCGTCTATGGTTTATGACAGGAAGA
3360
ori
3370 3380 3390
AGTGTAGCCGTAGTTAGGCCACCACTTCAA
TCACATCGGCATCAATCCGGTGGTGAAGTT
3390
ori
3400 3410 3420
GAACTCTGTAGCACCGCCTACATACCTCGC
CTTGAGACATCGTGGCGGATGTATGGAGCG
3420
ori
3430 3440 3450
TCTGCTAATCCTGTTACCAGTGGCTGCTGC
AGACGATTAGGACAATGGTCACCGACGACG
3450
ori
3460 3470 3480
CAGTGGCGATAAGTCGTGTCTTACCGGGTT
GTCACCGCTATTCAGCACAGAATGGCCCAA
3480
ori
3490 3500 3510
GGACTCAAGACGATAGTTACCGGATAAGGC
CCTGAGTTCTGCTATCAATGGCCTATTCCG
3510
ori
3520 3530 3540
GCAGCGGTCGGGCTGAACGGGGGGTTCGTG
CGTCGCCAGCCCGACTTGCCCCCCAAGCAC
3540
ori
3550 3560 3570
CACACAGCCCAGCTTGGAGCGAACGACCTA
GTGTGTCGGGTCGAACCTCGCTTGCTGGAT
3570
ori
3580 3590 3600
CACCGAACTGAGATACCTACAGCGTGAGCT
GTGGCTTGACTCTATGGATGTCGCACTCGA
3600
ori
3610 3620 3630
ATGAGAAAGCGCCACGCTTCCCGAAGGGAG
TACTCTTTCGCGGTGCGAAGGGCTTCCCTC
3630
ori
3640 3650 3660
AAAGGCGGACAGGTATCCGGTAAGCGGCAG
TTTCCGCCTGTCCATAGGCCATTCGCCGTC
3660
ori
3670 3680 3690
GGTCGGAACAGGAGAGCGCACGAGGGAGCT
CCAGCCTTGTCCTCTCGCGTGCTCCCTCGA
3690
ori
3700 3710 3720
TCCAGGGGGAAACGCCTGGTATCTTTATAG
AGGTCCCCCTTTGCGGACCATAGAAATATC
3720
ori
3730 3740 3750
TCCTGTCGGGTTTCGCCACCTCTGACTTGA
AGGACAGCCCAAAGCGGTGGAGACTGAACT
3750
ori
3760 3770 3780
GCGTCGATTTTTGTGATGCTCGTCAGGGGG
CGCAGCTAAAAACACTACGAGCAGTCCCCC
3780
ori
3790 3800 3810
GCGGAGCCTATGGAAAAACGCCAGCAACGC
CGCCTCGGATACCTTTTTGCGGTCGTTGCG
3810
ori
3820 3830 3840
GGCCTTTTTACGGTTCCTGGCCTTTTGCTG
CCGGAAAAATGCCAAGGACCGGAAAACGAC
3840
3850 3860 3870
GCCTTTTGCTCACATGTTCTTTCCTGCGTT
CGGAAAACGAGTGTACAAGAAAGGACGCAA
3870
3880 3890 3900
ATCCCCTGATTCTGTGGATAACCGTATTAC
TAGGGGACTAAGACACCTATTGGCATAATG
3900
3910 3920 3930
CGCCTTTGAGTGAGCTGATACCGCTCGCCG
GCGGAAACTCACTCGACTATGGCGAGCGGC
3930
3940 3950 3960
CAGCCGAACGACCGAGCGCAGCGAGTCAGT
GTCGGCTTGCTGGCTCGCGTCGCTCAGTCA
3960
3970 3980 3990
GAGCGAGGAAGCGGAAGAGCGCCTGATGCG
CTCGCTCCTTCGCCTTCTCGCGGACTACGC
3990
bom
4000 4010 4020
GTATTTTCTCCTTACGCATCTGTGCGGTAT
CATAAAAGAGGAATGCGTAGACACGCCATA
4020
bom
4030 4040 4050
TTCACACCGCATATATGGTGCACTCTCAGT
AAGTGTGGCGTATATACCACGTGAGAGTCA
4050
bom
4060 4070 4080
ACAATCTGCTCTGATGCCGCATAGTTAAGC
TGTTAGACGAGACTACGGCGTATCAATTCG
4080
bom
4090 4100 4110
CAGTATACACTCCGCTATCGCTACGTGACT
GTCATATGTGAGGCGATAGCGATGCACTGA
4110
bom
4120 4130 4140
GGGTCATGGCTGCGCCCCGACACCCGCCAA
CCCAGTACCGACGCGGGGCTGTGGGCGGTT
4140
bom
4150 4160 4170
CACCCGCTGACGCGCCCTGACGGGCTTGTC
GTGGGCGACTGCGCGGGACTGCCCGAACAG
4170
4180 4190 4200
TGCTCCCGGCATCCGCTTACAGACAAGCTG
ACGAGGGCCGTAGGCGAATGTCTGTTCGAC
4200
4210 4220 4230
TGACCGTCACCGGGAGCTGCATGTGTCAGA
ACTGGCAGTGGCCCTCGACGTACACAGTCT
4230
Leu
Ter
rop
4240 4250 4260
GGTTTTCACCGTCATCACCGAAACGCGCGA
CCAAAAGTGGCAGTAGTGGCTTTGCGCGCT
4260
Asn Glu Gly Asp Asp Gly Phe Arg Ala Leu
rop
4270 4280 4290
GGCAGCTGCGGTAAAGCTCATCAGCGTGGT
CCGTCGACGCCATTTCGAGTAGTCGCACCA
4290
Cys Ser Arg Tyr Leu Glu Asp Ala His Asp
rop
4300 4310 4320
CGTGAAGCGATTCACAGATGTCTGCCTGTT
GCACTTCGCTAAGTGTCTACAGACGGACAA
4320
His Leu Ser Glu Cys Ile Asp Ala Gln Glu
rop
4330 4340 4350
CATCCGCGTCCAGCTCGTTGAGTTTCTCCA
GTAGGCGCAGGTCGAGCAACTCAAAGAGGT
4350
Asp Ala Asp Leu Glu Asn Leu Lys Glu Leu
rop
4360 4370 4380
GAAGCGTTAATGTCTGGCTTCTGATAAAGC
CTTCGCAATTACAGACCGAAGACTATTTCG
4380
Leu Thr Leu Thr Gln Ser Arg Ile Phe Arg
rop
4390 4400 4410
GGGCCATGTTAAGGGCGGTTTTTTCCTGTT
CCCGGTACAATTCCCGCCAAAAAAGGACAA
4410
Ala Met Asn Leu Ala Thr Lys Glu Gln Lys
rop
4420 4430 4440
TGGTCACTGATGCCTCCGTGTAAGGGGGAT
ACCAGTGACTACGGAGGCACATTCCCCCTA
4440
Thr Val
rop
4450 4460 4470
TTCTGTTCATGGGGGTAATGATACCGATGA
AAGACAAGTACCCCCATTACTATGGCTACT
4470
4480 4490 4500
AACGAGAGAGGATGCTCACGATACGGGTTA
TTGCTCTCTCCTACGAGTGCTATGCCCAAT
4500
4510 4520 4530
CTGATGATGAACATGCCCGGTTACTGGAAC
GACTACTACTTGTACGGGCCAATGACCTTG
4530
4540 4550 4560
GTTGTGAGGGTAAACAACTGGCGGTATGGA
CAACACTCCCATTTGTTGACCGCCATACCT
4560
4570 4580 4590
TGCGGCGGGACCAGAGAAAAATCACTCAGG
ACGCCGCCCTGGTCTCTTTTTAGTGAGTCC
4590
4600 4610 4620
GTCAATGCCAGCGCTTCGTTAATACAGATG
CAGTTACGGTCGCGAAGCAATTATGTCTAC
4620
4630 4640 4650
TAGGTGTTCCACAGGGTAGCCAGCAGCATC
ATCCACAAGGTGTCCCATCGGTCGTCGTAG
4650
4660 4670 4680
CTGCGATGCAGATCCGGAACATAATGGTGC
GACGCTACGTCTAGGCCTTGTATTACCACG
4680
4690 4700 4710
AGGGCGCTGACTTCCGCGTTTCCAGACTTT
TCCCGCGACTGAAGGCGCAAAGGTCTGAAA
4710
4720 4730 4740
ACGAAACACGGAAACCAAAGACCATTCATG
TGCTTTGTGCCTTTGGTTTCTGGTAAGTAC
4740
4750 4760 4770
TTGTTGCTCAGGTCGCAGACGTTTTGCAGC
AACAACGAGTCCAGCGTCTGCAAAACGTCG
4770
4780 4790 4800
AGCAGTCGCTTCACGTTCGCTCGCGTATCG
TCGTCAGCGAAGTGCAAGCGAGCGCATAGC
4800
4810 4820 4830
GTGATTCATTCTGCTAACCAGTAAGGCAAC
CACTAAGTAAGACGATTGGTCATTCCGTTG
4830
4840 4850 4860
CCCGCCAGCCTAGCCGGGTCCTCAACGACA
GGGCGGTCGGATCGGCCCAGGAGTTGCTGT
4860
4870 4880 4890
GGAGCACGATCATGCGCACCCGTGGGGCCG
CCTCGTGCTAGTACGCGTGGGCACCCCGGC
4890
4900 4910 4920
CCATGCCGGCGATAATGGCCTGCTTCTCGC
GGTACGGCCGCTATTACCGGACGAAGAGCG
4920
4930 4940 4950
CGAAACGTTTGGTGGCGGGACCAGTGACGA
GCTTTGCAAACCACCGCCCTGGTCACTGCT
4950
4960 4970 4980
AGGCTTGAGCGAGGGCGTGCAAGATTCCGA
TCCGAACTCGCTCCCGCACGTTCTAAGGCT
4980
4990 5000 5010
ATACCGCAAGCGACAGGCCGATCATCGTCG
TATGGCGTTCGCTGTCCGGCTAGTAGCAGC
5010
5020 5030 5040
CGCTCCAGCGAAAGCGGTCCTCGCCGAAAA
GCGAGGTCGCTTTCGCCAGGAGCGGCTTTT
5040
5050 5060 5070
TGACCCAGAGCGCTGCCGGCACCTGTCCTA
ACTGGGTCTCGCGACGGCCGTGGACAGGAT
5070
5080 5090 5100
CGAGTTGCATGATAAAAAAGACAGTCATAA
GCTCAACGTACTATTTTTTCTGTCAGTATT
5100
5110 5120 5130
GTGCGGCGACGATAGTCATGCCCCGCGCCC
CACGCCGCTGCTATCAGTACGGGGCGCGGG
5130
5140 5150 5160
ACCGGAAGGAGCTGACTGGGTTGAAGGCTC
TGGCCTTCCTCGACTGACCCAACTTCCGAG
5160
5170 5180 5190
TCAAGGGCATCGGTCGAGATCCCGGTGCCT
AGTTCCCGTAGCCAGCTCTAGGGCCACGGA
5190
5200 5210 5220
AATGAGTGAGCTAACTTACATTAATTGCGT
TTACTCACTCGATTGAATGTAATTAACGCA
5220
5230 5240 5250
TGCGCTCACTGCCCGCTTTCCAGTCGGGAA
ACGCGAGTGACGGGCGAAAGGTCAGCCCTT
5250
Gln Gly Ser Glu Leu Arg Ser
Ter
lacI
5260 5270 5280
ACCTGTCGTGCCAGCTGCATTAATGAATCG
TGGACAGCACGGTCGACGTAATTACTTAGC
5280
Val Gln Arg Ala Leu Gln Met Leu Ser Asp
lacI
5290 5300 5310
GCCAACGCGCGGGGAGAGGCGGTTTGCGTA
CGGTTGCGCGCCCCTCTCCGCCAAACGCAT
5310
Ala Leu Ala Arg Pro Ser Ala Thr Gln Thr
lacI
5320 5330 5340
TTGGGCGCCAGGGTGGTTTTTCTTTTCACC
AACCCGCGGTCCCACCAAAAAGAAAAGTGG
5340
Asn Pro Ala Leu Thr Thr Lys Arg Lys Val
lacI
5350 5360 5370
AGTGAAACGGGCAACAGCTGATTGCCCTTC
TCACTTTGCCCGTTGTCGACTAACGGGAAG
5370
Leu Ser Val Pro Leu Leu Gln Asn Gly Lys
lacI
5380 5390 5400
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAG
TGGCGGACCGGGACTCTCTCAACGTCGTTC
5400
Val Ala Gln Gly Gln Ser Leu Gln Leu Leu
lacI
5410 5420 5430
CGGTCCACGCTGGTTTGCCCCAGCAGGCGA
GCCAGGTGCGACCAAACGGGGTCGTCCGCT
5430
Arg Asp Val Ser Thr Gln Gly Leu Leu Arg
lacI
5440 5450 5460
AAATCCTGTTTGATGGTGGTTAACGGCGGG
TTTAGGACAAACTACCACCAATTGCCGCCC
5460
Phe Asp Gln Lys Ile Thr Thr Leu Pro Pro
lacI
5470 5480 5490
ATATAACATGAGCTATCTTCGGTATCGTCG
TATATTGTACTCGATAGAAGCCATAGCAGC
5490
Ile Tyr Cys Ser Ser Asp Glu Thr Asp Asp
lacI
5500 5510 5520
TATCCCACTACCGAGATGTCCGCACCAACG
ATAGGGTGATGGCTCTACAGGCGTGGTTGC
5520
Tyr Gly Val Val Ser Ile Asp Ala Gly Val
lacI
5530 5540 5550
CGCAGCCCGGACTCGGTAATGGCGCGCATT
GCGTCGGGCCTGAGCCATTACCGCGCGTAA
5550
Arg Leu Gly Ser Glu Thr Ile Ala Arg Met
lacI
5560 5570 5580
GCGCCCAGCGCCATCTGATCGTTGGCAACC
CGCGGGTCGCGGTAGACTAGCAACCGTTGG
5580
Ala Gly Leu Ala Met Gln Asp Asn Ala Val
lacI
5590 5600 5610
AGCATCGCAGTGGGAACGATGCCCTCATTC
TCGTAGCGTCACCCTTGCTACGGGAGTAAG
5610
Leu Met Ala Thr Pro Val Ile Gly Glu Asn
lacI
5620 5630 5640
AGCATTTGCATGGTTTGTTGAAAACCGGAC
TCGTAAACGTACCAAACAACTTTTGGCCTG
5640
Leu Met Gln Met Thr Gln Gln Phe Gly Ser
lacI
5650 5660 5670
ATGGCACTCCAGTCGCCTTCCCGTTCCGCT
TACCGTGAGGTCAGCGGAAGGGCAAGGCGA
5670
Met Ala Ser Trp Asp Gly Glu Arg Glu Ala
lacI
5680 5690 5700
ATCGGCTGAATTTGATTGCGAGTGAGATAT
TAGCCGACTTAAACTAACGCTCACTCTATA
5700
Ile Pro Gln Ile Gln Asn Arg Thr Leu Tyr
lacI
5710 5720 5730
TTATGCCAGCCAGCCAGACGCAGACGCGCC
AATACGGTCGGTCGGTCTGCGTCTGCGCGG
5730
Lys His Trp Gly Ala Leu Arg Leu Arg Ala
lacI
5740 5750 5760
GAGACAGAACTTAATGGGCCCGCTAACAGC
CTCTGTCTTGAATTACCCGGGCGATTGTCG
5760
Ser Val Ser Ser Leu Pro Gly Ala Leu Leu
lacI
5770 5780 5790
GCGATTTGCTGGTGACCCAATGCGACCAGA
CGCTAAACGACCACTGGGTTACGCTGGTCT
5790
Ala Ile Gln Gln His Gly Leu Ala Val Leu
lacI
5800 5810 5820
TGCTCCACGCCCAGTCGCGTACCATCTTCA
ACGAGGTGCGGGTCAGCGCATGGTAGAAGT
5820
His Glu Val Gly Leu Arg Thr Gly Asp Glu
lacI
5830 5840 5850
TGGGAGAAAATAATACTGTTGATGGGTGTC
ACCCTCTTTTATTATGACAACTACCCACAG
5850
His Ser Phe Ile Ile Ser Asn Ile Pro Thr
lacI
5860 5870 5880
TGGTCAGAGACATCAAGAAATAACGCCGGA
ACCAGTCTCTGTAGTTCTTTATTGCGGCCT
5880
Gln Asp Ser Val Asp Leu Phe Leu Ala Pro
lacI
5890 5900 5910
ACATTAGTGCAGGCAGCTTCCACAGCAATG
TGTAATCACGTCCGTCGAAGGTGTCGTTAC
5910
Val Asn Thr Cys Ala Ala Glu Val Ala Ile
lacI
5920 5930 5940
GCATCCTGGTCATCCAGCGGATAGTTAATG
CGTAGGACCAGTAGGTCGCCTATCAATTAC
5940
Ala Asp Gln Asp Asp Leu Pro Tyr Asn Ile
lacI
5950 5960 5970
ATCAGCCCACTGACGCGTTGCGCGAGAAGA
TAGTCGGGTGACTGCGCAACGCGCTCTTCT
5970
Ile Leu Gly Ser Val Arg Gln Ala Leu Leu
lacI
5980 5990 6000
TTGTGCACCGCCGCTTTACAGGCTTCGACG
AACACGTGGCGGCGAAATGTCCGAAGCTGC
6000
Asn His Val Ala Ala Lys Cys Ala Glu Val
lacI
6010 6020 6030
CCGCTTCGTTCTACCATCGACACCACCACG
GGCGAAGCAAGATGGTAGCTGTGGTGGTGC
6030
Gly Ser Arg Glu Val Met Ser Val Val Val
lacI
6040 6050 6060
CTGGCACCCAGTTGATCGGCGCGAGATTTA
GACCGTGGGTCAACTAGCCGCGCTCTAAAT
6060
Ser Ala Gly Leu Gln Asp Ala Arg Ser Lys
lacI
6070 6080 6090
ATCGCCGCGACAATTTGCGACGGCGCGTGC
TAGCGGCGCTGTTAAACGCTGCCGCGCACG
6090
Ile Ala Ala Val Ile Gln Ser Pro Ala His
lacI
6100 6110 6120
AGGGCCAGACTGGAGGTGGCAACGCCAATC
TCCCGGTCTGACCTCCACCGTTGCGGTTAG
6120
Leu Ala Leu Ser Ser Thr Ala Val Gly Ile
lacI
6130 6140 6150
AGCAACGACTGTTTGCCCGCCAGTTGTTGT
TCGTTGCTGACAAACGGGCGGTCAACAACA
6150
Leu Leu Ser Gln Lys Gly Ala Leu Gln Gln
lacI
6160 6170 6180
GCCACGCGGTTGGGAATGTAATTCAGCTCC
CGGTGCGCCAACCCTTACATTAAGTCGAGG
6180
Ala Val Arg Asn Pro Ile Tyr Asn Leu Glu
lacI
6190 6200 6210
GCCATCGCCGCTTCCACTTTTTCCCGCGTT
CGGTAGCGGCGAAGGTGAAAAAGGGCGCAA
6210
Ala Met Ala Ala Glu Val Lys Glu Arg Thr
lacI
6220 6230 6240
TTCGCAGAAACGTGGCTGGCCTGGTTCACC
AAGCGTCTTTGCACCGACCGGACCAAGTGG
6240
Lys Ala Ser Val His Ser Ala Gln Asn Val
lacI
6250 6260 6270
ACGCGGGAAACGGTCTGATAAGAGACACCG
TGCGCCCTTTGCCAGACTATTCTCTGTGGC
6270
Val Arg Ser Val Thr Gln Tyr Ser Val Gly
lacI
6280 6290 6300
GCATACTCTGCGACATCGTATAACGTTACT
CGTATGAGACGCTGTAGCATATTGCAATGA
6300
Ala Tyr Glu Ala Val Asp Tyr Leu Thr Val
lacI
6310 6320 6330
GGTTTCACATTCACCACCCTGAATTGACTC
CCAAAGTGTAAGTGGTGGGACTTAACTGAG
6330
Pro Lys Val
lacI
lacI promoter
6340 6350 6360
TCTTCCGGGCGCTATCATGCCATACCGCGA
AGAAGGCCCGCGATAGTACGGTATGGCGCT
6360
lacI promoter
6370 6380 6390
AAGGTTTTGCGCCATTCGATGGTGTCCGGG
TTCCAAAACGCGGTAAGCTACCACAGGCCC
6390
lacI promoter
6400 6410 6420
ATCTCGACGCTCTCCCTTATGCGACTCCTG
TAGAGCTGCGAGAGGGAATACGCTGAGGAC
6420
6430
CATTAGGAAGCAGCC
GTAATCCTTCGTCGG
6435