Features
Cut sites
CDS
misc_feature
promoter
protein_bind
RBS
rep_origin
terminator
Circle
Line
Sequence
Zoom
Reset
1000
2000
3000
4000
5000
f1 ori
ori
lacI
rop
KanR
6xHis
T7 terminator
T7 promoter
RBS
lac operator
bom
lacI promoter
KanR promoter
internal stuffer
PmeI
PmeI
BbsI
-
BsaI
(370)
BbsI
(400)
BsaI
(404)
DMX-iGGA N
5393 bp
1000
2000
3000
4000
5000
f1 ori
ori
bom
lacI
lacI promoter
rop
KanR promoter
KanR
6xHis
T7 terminator
T7 promoter
RBS
lac operator
internal stuffer
PmeI
PmeI
BbsI
-
BsaI
(370)
BbsI
(400)
BsaI
(404)
DMX-iGGA N
5393 bp
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
f1 ori
ori
bom
lacI
lacI promoter
rop
KanR promoter
KanR
PmeI
6xHis
T7 terminator
T7 promoter
RBS
lac operator
internal stuffer
PmeI
BbsI
-
BsaI
(370)
BbsI
(400)
BsaI
(404)
DMX-iGGA N
5393 bp
200
400
600
800
1000
1200
1400
1600
1800
2000
2200
2400
2600
2800
3000
3200
3400
3600
3800
4000
4200
4400
4600
4800
5000
5200
f1 ori
ori
lac operator
bom
lacI
lacI promoter
rop
KanR promoter
KanR
PmeI
6xHis
T7 terminator
T7 promoter
RBS
internal stuffer
PmeI
BbsI
-
BsaI
(370)
BbsI
(400)
BsaI
(404)
DMX-iGGA N
5393 bp
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
2500
2600
2700
2800
2900
3000
3100
3200
3300
3400
3500
3600
3700
3800
3900
4000
4100
4200
4300
4400
4500
4600
4700
4800
4900
5000
5100
5200
5300
T7 terminator
T7 promoter
RBS
f1 ori
ori
lac operator
bom
lacI
lacI promoter
rop
KanR promoter
KanR
6xHis
internal stuffer
PmeI
PmeI
BbsI
-
BsaI
(370)
BbsI
(400)
BsaI
(404)
DMX-iGGA N
5393 bp
Both strands
Copy
10 20 30 40 50 60
CAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGAT
GTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90 100 110 120
GGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCT
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150 160 170 180
CATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCC
GTACTCGGGCTTCACCGCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210 220 230 240
AGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGA
TCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270 280 290 300
GATCGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAA
CTAGCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330 340 350 360
CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAGTTTAAACTA
GTTAAGGGGAGATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATGTCAAATTTGAT
360
lac operator
RBS
PmeI
370 380 390 400 410 420
TGGGGTCTCAAGGAAAGTCTTCAGCCCGGGCAGAAGACAATTCCTTCCGGAGACCACCAC
ACCCCAGAGTTCCTTTCAGAAGTCGGGCCCGTCTTCTGTTAAGGAAGGCCTCTGGTGGTG
420
internal stuffer
His His
6xHis
BbsI
BsaI
BbsI
BsaI
430 440 450 460 470 480
CACCACCACCACTGGTAGTTTAAACATGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
GTGGTGGTGGTGACCATCAAATTTGTACTCTAGGCCGACGATTGTTTCGGGCTTTCCTTC
480
His His His His
6xHis
PmeI
490 500 510 520 530 540
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAAC
GACTCAACCGACGACGGTGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTG
540
T7 terminator
550 560 570 580 590 600
GGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGC
CCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTAACCGCTTACCCTGCG
600
T7 terminator
f1 ori
610 620 630 640 650 660
GCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTAC
CGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATG
660
f1 ori
670 680 690 700 710 720
ACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTT
TGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAA
720
f1 ori
730 740 750 760 770 780
CGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGC
GCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACG
780
f1 ori
790 800 810 820 830 840
TTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATC
AAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAG
840
f1 ori
850 860 870 880 890 900
GCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACT
CGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGA
900
f1 ori
910 920 930 940 950 960
CTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGG
GAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCC
960
f1 ori
970 980 990 1000 1010 1020
GATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGC
CTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCG
1020
f1 ori
1030 1040 1050 1060 1070 1080
GAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCG
CTTAAAATTGTTTTATAATTGCAAATGTTAAAGTCCACCGTGAAAAGCCCCTTTACACGC
1080
f1 ori
1090 1100 1110 1120 1130 1140
CGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTA
GCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTTAAT
1140
1150 1160 1170 1180 1190 1200
ATTCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGG
TAAGAGTCTTCTTGAGCAGTTCTTCCGCTATCTTCCGCTACGCGACGCTTAGCCCTCGCC
1200
Phe Phe Glu Asp Leu Leu Arg Tyr Phe Ala Ile Arg Gln Ser Asp Pro Ala Ala
Ter
KanR
1210 1220 1230 1240 1250 1260
CGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATAT
GCTATGGCATTTCGTGCTCCTTCGCCAGTCGGGTAAGCGGCGGTTCGAGAAGTCGTTATA
1260
Ile Gly Tyr Leu Val Leu Phe Arg Asp Ala Trp Glu Gly Gly Leu Glu Glu Ala Ile Asp
KanR
1270 1280 1290 1300 1310 1320
CACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGA
GTGCCCATCGGTTGCGATACAGGACTATCGCCAGGCGGTGTGGGTCGGCCGGTGTCAGCT
1320
Arg Thr Ala Leu Ala Ile Asp Gln Tyr Arg Asp Ala Val Gly Leu Arg Gly Cys Asp Ile
KanR
1330 1340 1350 1360 1370 1380
TGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGG
ACTTAGGTCTTTTCGCCGGTAAAAGGTGGTACTATAAGCCGTTCGTCCGTAGCGGTACCC
1380
Phe Gly Ser Phe Arg Gly Asn Glu Val Met Ile Asn Pro Leu Cys Ala Asp Gly His Thr
KanR
1390 1400 1410 1420 1430 1440
TCACGACGAGATCCTCGCCGTCGGGCATGCTCGCCTTGAGCCTGGCGAACAGTTCGGCTG
AGTGCTGCTCTAGGAGCGGCAGCCCGTACGAGCGGAACTCGGACCGCTTGTCAAGCCGAC
1440
Val Val Leu Asp Glu Gly Asp Pro Met Ser Ala Lys Leu Arg Ala Phe Leu Glu Ala Pro
KanR
1450 1460 1470 1480 1490 1500
GCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCC
CGCGCTCGGGGACTACGAGAAGCAGGTCTAGTAGGACTAGCTGTTCTGGCCGAAGGTAGG
1500
Ala Leu Gly Gln His Glu Glu Asp Leu Asp Asp Gln Asp Val Leu Gly Ala Glu Met Arg
KanR
1510 1520 1530 1540 1550 1560
GAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGAT
CTCATGCACGAGCGAGCTACGCTACAAAGCGAACCACCAGCTTACCCGTCCATCGGCCTA
1560
Thr Arg Ala Arg Glu Ile Arg His Lys Ala Gln His Asp Phe Pro Cys Thr Ala Pro Asp
KanR
1570 1580 1590 1600 1610 1620
CAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAA
GTTCGCATACGTCGGCGGCGTAACGTAGTCGGTACTACCTATGAAAGAGCCGTCCTCGTT
1620
Leu Thr His Leu Arg Arg Met Ala Asp Ala Met Ile Ser Val Lys Glu Ala Pro Ala Leu
KanR
1630 1640 1650 1660 1670 1680
GGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCG
CCACTCTACTGTCCTCTAGGACGGGGCCGTGAAGCGGGTTATCGTCGGTCAGGGAAGGGC
1680
His Ser Ser Leu Leu Asp Gln Gly Pro Val Glu Gly Leu Leu Leu Trp Asp Arg Gly Ala
KanR
1690 1700 1710 1720 1730 1740
CTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATA
GAAGTCACTGTTGCAGCTCGTGTCGACGCGTTCCTTGCGGGCAGCACCGGTCGGTGCTAT
1740
Glu Thr Val Val Asp Leu Val Ala Ala Cys Pro Val Gly Thr Thr Ala Leu Trp Ser Leu
KanR
1750 1760 1770 1780 1790 1800
GCCGCGCTGCCTCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAA
CGGCGCGACGGAGCAGAACGTCAAGTAAGTCCCGTGGCCTGTCCAGCCAGAACTGTTTTT
1800
Arg Ala Ala Glu Asp Gln Leu Glu Asn Leu Ala Gly Ser Leu Asp Thr Lys Val Phe Leu
KanR
1810 1820 1830 1840 1850 1860
GAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCT
CTTGGCCCGCGGGGACGCGACTGTCGGCCTTGTGCCGCCGTAGTCTCGTCGGCTAACAGA
1860
Val Pro Arg Gly Gln Ala Ser Leu Arg Phe Val Ala Ala Asp Ser Cys Gly Ile Thr Gln
KanR
1870 1880 1890 1900 1910 1920
GTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCA
CAACACGGGTCAGTATCGGCTTATCGGAGAGGTGGGTTCGCCGGCCTCTTGGACGCACGT
1920
Gln Ala Trp Asp Tyr Gly Phe Leu Arg Glu Val Trp Ala Ala Pro Ser Gly Ala His Leu
KanR
1930 1940 1950 1960 1970 1980
ATCCATCTTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT
TAGGTAGAACAAGTTAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAA
1980
Gly Asp Gln Glu Ile Met
KanR
KanR promoter
1990 2000 2010 2020 2030 2040
ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTC
TAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAG
2040
KanR promoter
2050 2060 2070 2080 2090 2100
CGCGAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAA
GCGCTTGTGGGGAACATAATGACAAATACATTCGTCTGTCAAAATAACAAGTACTGGTTT
2100
KanR promoter
2110 2120 2130 2140 2150 2160
ATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGA
TAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCT
2160
2170 2180 2190 2200 2210 2220
TCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG
AGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGC
2220
ori
2230 2240 2250 2260 2270 2280
CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACT
GATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGA
2280
ori
2290 2300 2310 2320 2330 2340
GGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCAC
CCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTG
2340
ori
2350 2360 2370 2380 2390 2400
CACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTG
GTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCAC
2400
ori
2410 2420 2430 2440 2450 2460
GCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG
CGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGC
2460
ori
2470 2480 2490 2500 2510 2520
GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGA
CTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCT
2520
ori
2530 2540 2550 2560 2570 2580
ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCC
TGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGG
2580
ori
2590 2600 2610 2620 2630 2640
GAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACG
CTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGC
2640
ori
2650 2660 2670 2680 2690 2700
AGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC
TCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAG
2700
ori
2710 2720 2730 2740 2750 2760
TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCC
ACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGG
2760
ori
2770 2780 2790 2800 2810 2820
AGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTT
TCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAA
2820
2830 2840 2850 2860 2870 2880
CCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC
GGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGG
2880
2890 2900 2910 2920 2930 2940
GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGC
CGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCG
2940
bom
2950 2960 2970 2980 2990 3000
CTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCA
GACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATATACCACGT
3000
bom
3010 3020 3030 3040 3050 3060
CTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCT
GAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGA
3060
bom
3070 3080 3090 3100 3110 3120
ACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACG
TGCACTGACCCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGC
3120
bom
3130 3140 3150 3160 3170 3180
GGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCACCGGGAGCTGCAT
CCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGTGGCCCTCGACGTA
3180
3190 3200 3210 3220 3230 3240
GTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATC
CACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAG
3240
Leu Asn Glu Gly Asp Asp Gly Phe Arg Ala Leu Cys Ser Arg Tyr Leu Glu Asp
Ter
rop
3250 3260 3270 3280 3290 3300
AGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAG
TCGCACCAGCACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGCAACTC
3300
Ala His Asp His Leu Ser Glu Cys Ile Asp Ala Gln Glu Asp Ala Asp Leu Glu Asn Leu
rop
3310 3320 3330 3340 3350 3360
TTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTT
AAAGAGGTCTTCGCAATTACAGACCGAAGACTATTTCGCCCGGTACAATTCCCGCCAAAA
3360
Lys Glu Leu Leu Thr Leu Thr Gln Ser Arg Ile Phe Arg Ala Met Asn Leu Ala Thr Lys
rop
3370 3380 3390 3400 3410 3420
TTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGAT
AAGGACAAACCAGTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTA
3420
Glu Gln Lys Thr Val
rop
3430 3440 3450 3460 3470 3480
ACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTT
TGGCTACTTTGCTCTCTCCTACGAGTGCTATGCCCAATGACTACTACTTGTACGGGCCAA
3480
3490 3500 3510 3520 3530 3540
ACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAAT
TGACCTTGCAACACTCCCATTTGTTGACCGCCATACCTACGCCGCCCTGGTCTCTTTTTA
3540
3550 3560 3570 3580 3590 3600
CACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCA
GTGAGTCCCAGTTACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGT
3600
3610 3620 3630 3640 3650 3660
GCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTC
CGTCGTAGGACGCTACGTCTAGGCCTTGTATTACCACGTCCCGCGACTGAAGGCGCAAAG
3660
3670 3680 3690 3700 3710 3720
CAGACTTTACGAAACACGGAAACCAAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGT
GTCTGAAATGCTTTGTGCCTTTGGTTTCTGGTAAGTACAACAACGAGTCCAGCGTCTGCA
3720
3730 3740 3750 3760 3770 3780
TTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGT
AAACGTCGTCGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCA
3780
3790 3800 3810 3820 3830 3840
AAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCG
TTCCGTTGGGGCGGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTGCTAGTACGCGTGGGC
3840
3850 3860 3870 3880 3890 3900
TGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACC
ACCCCGGCGGTACGGCCGCTATTACCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGG
3900
3910 3920 3930 3940 3950 3960
AGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGAT
TCACTGCTTCCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTA
3960
3970 3980 3990 4000 4010 4020
CATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCAC
GTAGCAGCGCGAGGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGTCTCGCGACGGCCGTG
4020
4030 4040 4050 4060 4070 4080
CTGTCCTACGAGTTGCATGATAAAAAAGACAGTCATAAGTGCGGCGACGATAGTCATGCC
GACAGGATGCTCAACGTACTATTTTTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGG
4080
4090 4100 4110 4120 4130 4140
CCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCC
GGCGCGGGTGGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGG
4140
4150 4160 4170 4180 4190 4200
CGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCA
GCCACGGATTACTCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGAAAGGT
4200
Gln Gly Ser Glu Leu
Ter
lacI
4210 4220 4230 4240 4250 4260
GTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG
CAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCC
4260
Arg Ser Val Gln Arg Ala Leu Gln Met Leu Ser Asp Ala Leu Ala Arg Pro Ser Ala Thr
lacI
4270 4280 4290 4300 4310 4320
TTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAAACGGGCAACAGCTGAT
AAACGCATAACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTTTGCCCGTTGTCGACTA
4320
Gln Thr Asn Pro Ala Leu Thr Thr Lys Arg Lys Val Leu Ser Val Pro Leu Leu Gln Asn
lacI
4330 4340 4350 4360 4370 4380
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
ACGGGAAGTGGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGT
4380
Gly Lys Val Ala Gln Gly Gln Ser Leu Gln Leu Leu Arg Asp Val Ser Thr Gln Gly Leu
lacI
4390 4400 4410 4420 4430 4440
GCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTATCTTCGG
CGTCCGCTTTTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGATAGAAGCC
4440
Leu Arg Phe Asp Gln Lys Ile Thr Thr Leu Pro Pro Ile Tyr Cys Ser Ser Asp Glu Thr
lacI
4450 4460 4470 4480 4490 4500
TATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGG
ATAGCAGCATAGGGTGATGGCTCTACAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACC
4500
Asp Asp Tyr Gly Val Val Ser Ile Asp Ala Gly Val Arg Leu Gly Ser Glu Thr Ile Ala
lacI
4510 4520 4530 4540 4550 4560
CGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGC
GCGCGTAACGCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACG
4560
Arg Met Ala Gly Leu Ala Met Gln Asp Asn Ala Val Leu Met Ala Thr Pro Val Ile Gly
lacI
4570 4580 4590 4600 4610 4620
CCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCC
GGAGTAAGTCGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGG
4620
Glu Asn Leu Met Gln Met Thr Gln Gln Phe Gly Ser Met Ala Ser Trp Asp Gly Glu Arg
lacI
4630 4640 4650 4660 4670 4680
GTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCA
CAAGGCGATAGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGT
4680
Glu Ala Ile Pro Gln Ile Gln Asn Arg Thr Leu Tyr Lys His Trp Gly Ala Leu Arg Leu
lacI
4690 4700 4710 4720 4730 4740
GACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATG
CTGCGCGGCTCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTAC
4740
Arg Ala Ser Val Ser Ser Leu Pro Gly Ala Leu Leu Ala Ile Gln Gln His Gly Leu Ala
lacI
4750 4760 4770 4780 4790 4800
CGACCAGATGCTCCACGCCCAGTCGCGTACCATCTTCATGGGAGAAAATAATACTGTTGA
GCTGGTCTACGAGGTGCGGGTCAGCGCATGGTAGAAGTACCCTCTTTTATTATGACAACT
4800
Val Leu His Glu Val Gly Leu Arg Thr Gly Asp Glu His Ser Phe Ile Ile Ser Asn Ile
lacI
4810 4820 4830 4840 4850 4860
TGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCA
ACCCACAGACCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGT
4860
Pro Thr Gln Asp Ser Val Asp Leu Phe Leu Ala Pro Val Asn Thr Cys Ala Ala Glu Val
lacI
4870 4880 4890 4900 4910 4920
CAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCG
GTCGTTACCGTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGCAACGC
4920
Ala Ile Ala Asp Gln Asp Asp Leu Pro Tyr Asn Ile Ile Leu Gly Ser Val Arg Gln Ala
lacI
4930 4940 4950 4960 4970 4980
CGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACA
GCTCTTCTAACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGT
4980
Leu Leu Asn His Val Ala Ala Lys Cys Ala Glu Val Gly Ser Arg Glu Val Met Ser Val
lacI
4990 5000 5010 5020 5030 5040
CCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACG
GGTGGTGCGACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGC
5040
Val Val Ser Ala Gly Leu Gln Asp Ala Arg Ser Lys Ile Ala Ala Val Ile Gln Ser Pro
lacI
5050 5060 5070 5080 5090 5100
GCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCA
CGCGCACGTCCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGGT
5100
Ala His Leu Ala Leu Ser Ser Thr Ala Val Gly Ile Leu Leu Ser Gln Lys Gly Ala Leu
lacI
5110 5120 5130 5140 5150 5160
GTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTT
CAACAACACGGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAA
5160
Gln Gln Ala Val Arg Asn Pro Ile Tyr Asn Leu Glu Ala Met Ala Ala Glu Val Lys Glu
lacI
5170 5180 5190 5200 5210 5220
CCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAG
GGGCGCAAAAGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTC
5220
Arg Thr Lys Ala Ser Val His Ser Ala Gln Asn Val Val Arg Ser Val Thr Gln Tyr Ser
lacI
5230 5240 5250 5260 5270 5280
AGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGA
TCTGTGGCCGTATGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACT
5280
Val Gly Ala Tyr Glu Ala Val Asp Tyr Leu Thr Val Pro Lys Val
lacI
lacI promoter
5290 5300 5310 5320 5330 5340
ATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGG
TAACTGAGAGAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACC
5340
lacI promoter
5350 5360 5370 5380 5390
TGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCC
ACAGGCCCTAGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGG
5393
lacI promoter
10 20 30
CAGTAGTAGGTTGAGGCCGTTGAGCACCGC
GTCATCATCCAACTCCGGCAACTCGTGGCG
30
40 50 60
CGCCGCAAGGAATGGTGCATGCAAGGAGAT
GCGGCGTTCCTTACCACGTACGTTCCTCTA
60
70 80 90
GGCGCCCAACAGTCCCCCGGCCACGGGGCC
CCGCGGGTTGTCAGGGGGCCGGTGCCCCGG
90
100 110 120
TGCCACCATACCCACGCCGAAACAAGCGCT
ACGGTGGTATGGGTGCGGCTTTGTTCGCGA
120
130 140 150
CATGAGCCCGAAGTGGCGAGCCCGATCTTC
GTACTCGGGCTTCACCGCTCGGGCTAGAAG
150
160 170 180
CCCATCGGTGATGTCGGCGATATAGGCGCC
GGGTAGCCACTACAGCCGCTATATCCGCGG
180
190 200 210
AGCAACCGCACCTGTGGCGCCGGTGATGCC
TCGTTGGCGTGGACACCGCGGCCACTACGG
210
220 230 240
GGCCACGATGCGTCCGGCGTAGAGGATCGA
CCGGTGCTACGCAGGCCGCATCTCCTAGCT
240
250 260 270
GATCGATCTCGATCCCGCGAAATTAATACG
CTAGCTAGAGCTAGGGCGCTTTAATTATGC
270
T7 promoter
280 290 300
ACTCACTATAGGGGAATTGTGAGCGGATAA
TGAGTGATATCCCCTTAACACTCGCCTATT
300
T7 promoter
lac operator
310 320 330
CAATTCCCCTCTAGAAATAATTTTGTTTAA
GTTAAGGGGAGATCTTTATTAAAACAAATT
330
lac operator
RBS
340 350 360
CTTTAAGAAGGAGATATACAGTTTAAACTA
GAAATTCTTCCTCTATATGTCAAATTTGAT
360
RBS
PmeI
370 380 390
TGGGGTCTCAAGGAAAGTCTTCAGCCCGGG
ACCCCAGAGTTCCTTTCAGAAGTCGGGCCC
390
internal stuffer
BbsI
BsaI
400 410 420
CAGAAGACAATTCCTTCCGGAGACCACCAC
GTCTTCTGTTAAGGAAGGCCTCTGGTGGTG
420
internal stuffer
His His
6xHis
BbsI
BsaI
430 440 450
CACCACCACCACTGGTAGTTTAAACATGAG
GTGGTGGTGGTGACCATCAAATTTGTACTC
450
His His His His
6xHis
PmeI
460 470 480
ATCCGGCTGCTAACAAAGCCCGAAAGGAAG
TAGGCCGACGATTGTTTCGGGCTTTCCTTC
480
490 500 510
CTGAGTTGGCTGCTGCCACCGCTGAGCAAT
GACTCAACCGACGACGGTGGCGACTCGTTA
510
520 530 540
AACTAGCATAACCCCTTGGGGCCTCTAAAC
TTGATCGTATTGGGGAACCCCGGAGATTTG
540
T7 terminator
550 560 570
GGGTCTTGAGGGGTTTTTTGCTGAAAGGAG
CCCAGAACTCCCCAAAAAACGACTTTCCTC
570
T7 terminator
580 590 600
GAACTATATCCGGATTGGCGAATGGGACGC
CTTGATATAGGCCTAACCGCTTACCCTGCG
600
f1 ori
610 620 630
GCCCTGTAGCGGCGCATTAAGCGCGGCGGG
CGGGACATCGCCGCGTAATTCGCGCCGCCC
630
f1 ori
640 650 660
TGTGGTGGTTACGCGCAGCGTGACCGCTAC
ACACCACCAATGCGCGTCGCACTGGCGATG
660
f1 ori
670 680 690
ACTTGCCAGCGCCCTAGCGCCCGCTCCTTT
TGAACGGTCGCGGGATCGCGGGCGAGGAAA
690
f1 ori
700 710 720
CGCTTTCTTCCCTTCCTTTCTCGCCACGTT
GCGAAAGAAGGGAAGGAAAGAGCGGTGCAA
720
f1 ori
730 740 750
CGCCGGCTTTCCCCGTCAAGCTCTAAATCG
GCGGCCGAAAGGGGCAGTTCGAGATTTAGC
750
f1 ori
760 770 780
GGGGCTCCCTTTAGGGTTCCGATTTAGTGC
CCCCGAGGGAAATCCCAAGGCTAAATCACG
780
f1 ori
790 800 810
TTTACGGCACCTCGACCCCAAAAAACTTGA
AAATGCCGTGGAGCTGGGGTTTTTTGAACT
810
f1 ori
820 830 840
TTAGGGTGATGGTTCACGTAGTGGGCCATC
AATCCCACTACCAAGTGCATCACCCGGTAG
840
f1 ori
850 860 870
GCCCTGATAGACGGTTTTTCGCCCTTTGAC
CGGGACTATCTGCCAAAAAGCGGGAAACTG
870
f1 ori
880 890 900
GTTGGAGTCCACGTTCTTTAATAGTGGACT
CAACCTCAGGTGCAAGAAATTATCACCTGA
900
f1 ori
910 920 930
CTTGTTCCAAACTGGAACAACACTCAACCC
GAACAAGGTTTGACCTTGTTGTGAGTTGGG
930
f1 ori
940 950 960
TATCTCGGTCTATTCTTTTGATTTATAAGG
ATAGAGCCAGATAAGAAAACTAAATATTCC
960
f1 ori
970 980 990
GATTTTGCCGATTTCGGCCTATTGGTTAAA
CTAAAACGGCTAAAGCCGGATAACCAATTT
990
f1 ori
1000 1010 1020
AAATGAGCTGATTTAACAAAAATTTAACGC
TTTACTCGACTAAATTGTTTTTAAATTGCG
1020
f1 ori
1030 1040 1050
GAATTTTAACAAAATATTAACGTTTACAAT
CTTAAAATTGTTTTATAATTGCAAATGTTA
1050
f1 ori
1060 1070 1080
TTCAGGTGGCACTTTTCGGGGAAATGTGCG
AAGTCCACCGTGAAAAGCCCCTTTACACGC
1080
f1 ori
1090 1100 1110
CGGAACCCCTATTTGTTTATTTTTCTAAAT
GCCTTGGGGATAAACAAATAAAAAGATTTA
1110
1120 1130 1140
ACATTCAAATATGTATCCGCTCATGAATTA
TGTAAGTTTATACATAGGCGAGTACTTAAT
1140
1150 1160 1170
ATTCTCAGAAGAACTCGTCAAGAAGGCGAT
TAAGAGTCTTCTTGAGCAGTTCTTCCGCTA
1170
Phe Phe Glu Asp Leu Leu Arg Tyr
Ter
KanR
1180 1190 1200
AGAAGGCGATGCGCTGCGAATCGGGAGCGG
TCTTCCGCTACGCGACGCTTAGCCCTCGCC
1200
Phe Ala Ile Arg Gln Ser Asp Pro Ala Ala
KanR
1210 1220 1230
CGATACCGTAAAGCACGAGGAAGCGGTCAG
GCTATGGCATTTCGTGCTCCTTCGCCAGTC
1230
Ile Gly Tyr Leu Val Leu Phe Arg Asp Ala
KanR
1240 1250 1260
CCCATTCGCCGCCAAGCTCTTCAGCAATAT
GGGTAAGCGGCGGTTCGAGAAGTCGTTATA
1260
Trp Glu Gly Gly Leu Glu Glu Ala Ile Asp
KanR
1270 1280 1290
CACGGGTAGCCAACGCTATGTCCTGATAGC
GTGCCCATCGGTTGCGATACAGGACTATCG
1290
Arg Thr Ala Leu Ala Ile Asp Gln Tyr Arg
KanR
1300 1310 1320
GGTCCGCCACACCCAGCCGGCCACAGTCGA
CCAGGCGGTGTGGGTCGGCCGGTGTCAGCT
1320
Asp Ala Val Gly Leu Arg Gly Cys Asp Ile
KanR
1330 1340 1350
TGAATCCAGAAAAGCGGCCATTTTCCACCA
ACTTAGGTCTTTTCGCCGGTAAAAGGTGGT
1350
Phe Gly Ser Phe Arg Gly Asn Glu Val Met
KanR
1360 1370 1380
TGATATTCGGCAAGCAGGCATCGCCATGGG
ACTATAAGCCGTTCGTCCGTAGCGGTACCC
1380
Ile Asn Pro Leu Cys Ala Asp Gly His Thr
KanR
1390 1400 1410
TCACGACGAGATCCTCGCCGTCGGGCATGC
AGTGCTGCTCTAGGAGCGGCAGCCCGTACG
1410
Val Val Leu Asp Glu Gly Asp Pro Met Ser
KanR
1420 1430 1440
TCGCCTTGAGCCTGGCGAACAGTTCGGCTG
AGCGGAACTCGGACCGCTTGTCAAGCCGAC
1440
Ala Lys Leu Arg Ala Phe Leu Glu Ala Pro
KanR
1450 1460 1470
GCGCGAGCCCCTGATGCTCTTCGTCCAGAT
CGCGCTCGGGGACTACGAGAAGCAGGTCTA
1470
Ala Leu Gly Gln His Glu Glu Asp Leu Asp
KanR
1480 1490 1500
CATCCTGATCGACAAGACCGGCTTCCATCC
GTAGGACTAGCTGTTCTGGCCGAAGGTAGG
1500
Asp Gln Asp Val Leu Gly Ala Glu Met Arg
KanR
1510 1520 1530
GAGTACGTGCTCGCTCGATGCGATGTTTCG
CTCATGCACGAGCGAGCTACGCTACAAAGC
1530
Thr Arg Ala Arg Glu Ile Arg His Lys Ala
KanR
1540 1550 1560
CTTGGTGGTCGAATGGGCAGGTAGCCGGAT
GAACCACCAGCTTACCCGTCCATCGGCCTA
1560
Gln His Asp Phe Pro Cys Thr Ala Pro Asp
KanR
1570 1580 1590
CAAGCGTATGCAGCCGCCGCATTGCATCAG
GTTCGCATACGTCGGCGGCGTAACGTAGTC
1590
Leu Thr His Leu Arg Arg Met Ala Asp Ala
KanR
1600 1610 1620
CCATGATGGATACTTTCTCGGCAGGAGCAA
GGTACTACCTATGAAAGAGCCGTCCTCGTT
1620
Met Ile Ser Val Lys Glu Ala Pro Ala Leu
KanR
1630 1640 1650
GGTGAGATGACAGGAGATCCTGCCCCGGCA
CCACTCTACTGTCCTCTAGGACGGGGCCGT
1650
His Ser Ser Leu Leu Asp Gln Gly Pro Val
KanR
1660 1670 1680
CTTCGCCCAATAGCAGCCAGTCCCTTCCCG
GAAGCGGGTTATCGTCGGTCAGGGAAGGGC
1680
Glu Gly Leu Leu Leu Trp Asp Arg Gly Ala
KanR
1690 1700 1710
CTTCAGTGACAACGTCGAGCACAGCTGCGC
GAAGTCACTGTTGCAGCTCGTGTCGACGCG
1710
Glu Thr Val Val Asp Leu Val Ala Ala Cys
KanR
1720 1730 1740
AAGGAACGCCCGTCGTGGCCAGCCACGATA
TTCCTTGCGGGCAGCACCGGTCGGTGCTAT
1740
Pro Val Gly Thr Thr Ala Leu Trp Ser Leu
KanR
1750 1760 1770
GCCGCGCTGCCTCGTCTTGCAGTTCATTCA
CGGCGCGACGGAGCAGAACGTCAAGTAAGT
1770
Arg Ala Ala Glu Asp Gln Leu Glu Asn Leu
KanR
1780 1790 1800
GGGCACCGGACAGGTCGGTCTTGACAAAAA
CCCGTGGCCTGTCCAGCCAGAACTGTTTTT
1800
Ala Gly Ser Leu Asp Thr Lys Val Phe Leu
KanR
1810 1820 1830
GAACCGGGCGCCCCTGCGCTGACAGCCGGA
CTTGGCCCGCGGGGACGCGACTGTCGGCCT
1830
Val Pro Arg Gly Gln Ala Ser Leu Arg Phe
KanR
1840 1850 1860
ACACGGCGGCATCAGAGCAGCCGATTGTCT
TGTGCCGCCGTAGTCTCGTCGGCTAACAGA
1860
Val Ala Ala Asp Ser Cys Gly Ile Thr Gln
KanR
1870 1880 1890
GTTGTGCCCAGTCATAGCCGAATAGCCTCT
CAACACGGGTCAGTATCGGCTTATCGGAGA
1890
Gln Ala Trp Asp Tyr Gly Phe Leu Arg Glu
KanR
1900 1910 1920
CCACCCAAGCGGCCGGAGAACCTGCGTGCA
GGTGGGTTCGCCGGCCTCTTGGACGCACGT
1920
Val Trp Ala Ala Pro Ser Gly Ala His Leu
KanR
1930 1940 1950
ATCCATCTTGTTCAATCATACTCTTCCTTT
TAGGTAGAACAAGTTAGTATGAGAAGGAAA
1950
Gly Asp Gln Glu Ile Met
KanR
KanR promoter
1960 1970 1980
TTCAATATTATTGAAGCATTTATCAGGGTT
AAGTTATAATAACTTCGTAAATAGTCCCAA
1980
KanR promoter
1990 2000 2010
ATTGTCTCATGAGCGGATACATATTTGAAT
TAACAGAGTACTCGCCTATGTATAAACTTA
2010
KanR promoter
2020 2030 2040
GTATTTAGAAAAATAAACAAATAGGGGTTC
CATAAATCTTTTTATTTGTTTATCCCCAAG
2040
KanR promoter
2050 2060 2070
CGCGAACACCCCTTGTATTACTGTTTATGT
GCGCTTGTGGGGAACATAATGACAAATACA
2070
KanR promoter
2080 2090 2100
AAGCAGACAGTTTTATTGTTCATGACCAAA
TTCGTCTGTCAAAATAACAAGTACTGGTTT
2100
2110 2120 2130
ATCCCTTAACGTGAGTTTTCGTTCCACTGA
TAGGGAATTGCACTCAAAAGCAAGGTGACT
2130
2140 2150 2160
GCGTCAGACCCCGTAGAAAAGATCAAAGGA
CGCAGTCTGGGGCATCTTTTCTAGTTTCCT
2160
2170 2180 2190
TCTTCTTGAGATCCTTTTTTTCTGCGCGTA
AGAAGAACTCTAGGAAAAAAAGACGCGCAT
2190
ori
2200 2210 2220
ATCTGCTGCTTGCAAACAAAAAAACCACCG
TAGACGACGAACGTTTGTTTTTTTGGTGGC
2220
ori
2230 2240 2250
CTACCAGCGGTGGTTTGTTTGCCGGATCAA
GATGGTCGCCACCAAACAAACGGCCTAGTT
2250
ori
2260 2270 2280
GAGCTACCAACTCTTTTTCCGAAGGTAACT
CTCGATGGTTGAGAAAAAGGCTTCCATTGA
2280
ori
2290 2300 2310
GGCTTCAGCAGAGCGCAGATACCAAATACT
CCGAAGTCGTCTCGCGTCTATGGTTTATGA
2310
ori
2320 2330 2340
GTCCTTCTAGTGTAGCCGTAGTTAGGCCAC
CAGGAAGATCACATCGGCATCAATCCGGTG
2340
ori
2350 2360 2370
CACTTCAAGAACTCTGTAGCACCGCCTACA
GTGAAGTTCTTGAGACATCGTGGCGGATGT
2370
ori
2380 2390 2400
TACCTCGCTCTGCTAATCCTGTTACCAGTG
ATGGAGCGAGACGATTAGGACAATGGTCAC
2400
ori
2410 2420 2430
GCTGCTGCCAGTGGCGATAAGTCGTGTCTT
CGACGACGGTCACCGCTATTCAGCACAGAA
2430
ori
2440 2450 2460
ACCGGGTTGGACTCAAGACGATAGTTACCG
TGGCCCAACCTGAGTTCTGCTATCAATGGC
2460
ori
2470 2480 2490
GATAAGGCGCAGCGGTCGGGCTGAACGGGG
CTATTCCGCGTCGCCAGCCCGACTTGCCCC
2490
ori
2500 2510 2520
GGTTCGTGCACACAGCCCAGCTTGGAGCGA
CCAAGCACGTGTGTCGGGTCGAACCTCGCT
2520
ori
2530 2540 2550
ACGACCTACACCGAACTGAGATACCTACAG
TGCTGGATGTGGCTTGACTCTATGGATGTC
2550
ori
2560 2570 2580
CGTGAGCTATGAGAAAGCGCCACGCTTCCC
GCACTCGATACTCTTTCGCGGTGCGAAGGG
2580
ori
2590 2600 2610
GAAGGGAGAAAGGCGGACAGGTATCCGGTA
CTTCCCTCTTTCCGCCTGTCCATAGGCCAT
2610
ori
2620 2630 2640
AGCGGCAGGGTCGGAACAGGAGAGCGCACG
TCGCCGTCCCAGCCTTGTCCTCTCGCGTGC
2640
ori
2650 2660 2670
AGGGAGCTTCCAGGGGGAAACGCCTGGTAT
TCCCTCGAAGGTCCCCCTTTGCGGACCATA
2670
ori
2680 2690 2700
CTTTATAGTCCTGTCGGGTTTCGCCACCTC
GAAATATCAGGACAGCCCAAAGCGGTGGAG
2700
ori
2710 2720 2730
TGACTTGAGCGTCGATTTTTGTGATGCTCG
ACTGAACTCGCAGCTAAAAACACTACGAGC
2730
ori
2740 2750 2760
TCAGGGGGGCGGAGCCTATGGAAAAACGCC
AGTCCCCCCGCCTCGGATACCTTTTTGCGG
2760
ori
2770 2780 2790
AGCAACGCGGCCTTTTTACGGTTCCTGGCC
TCGTTGCGCCGGAAAAATGCCAAGGACCGG
2790
2800 2810 2820
TTTTGCTGGCCTTTTGCTCACATGTTCTTT
AAAACGACCGGAAAACGAGTGTACAAGAAA
2820
2830 2840 2850
CCTGCGTTATCCCCTGATTCTGTGGATAAC
GGACGCAATAGGGGACTAAGACACCTATTG
2850
2860 2870 2880
CGTATTACCGCCTTTGAGTGAGCTGATACC
GCATAATGGCGGAAACTCACTCGACTATGG
2880
2890 2900 2910
GCTCGCCGCAGCCGAACGACCGAGCGCAGC
CGAGCGGCGTCGGCTTGCTGGCTCGCGTCG
2910
2920 2930 2940
GAGTCAGTGAGCGAGGAAGCGGAAGAGCGC
CTCAGTCACTCGCTCCTTCGCCTTCTCGCG
2940
bom
2950 2960 2970
CTGATGCGGTATTTTCTCCTTACGCATCTG
GACTACGCCATAAAAGAGGAATGCGTAGAC
2970
bom
2980 2990 3000
TGCGGTATTTCACACCGCATATATGGTGCA
ACGCCATAAAGTGTGGCGTATATACCACGT
3000
bom
3010 3020 3030
CTCTCAGTACAATCTGCTCTGATGCCGCAT
GAGAGTCATGTTAGACGAGACTACGGCGTA
3030
bom
3040 3050 3060
AGTTAAGCCAGTATACACTCCGCTATCGCT
TCAATTCGGTCATATGTGAGGCGATAGCGA
3060
bom
3070 3080 3090
ACGTGACTGGGTCATGGCTGCGCCCCGACA
TGCACTGACCCAGTACCGACGCGGGGCTGT
3090
bom
3100 3110 3120
CCCGCCAACACCCGCTGACGCGCCCTGACG
GGGCGGTTGTGGGCGACTGCGCGGGACTGC
3120
3130 3140 3150
GGCTTGTCTGCTCCCGGCATCCGCTTACAG
CCGAACAGACGAGGGCCGTAGGCGAATGTC
3150
3160 3170 3180
ACAAGCTGTGACCGTCACCGGGAGCTGCAT
TGTTCGACACTGGCAGTGGCCCTCGACGTA
3180
3190 3200 3210
GTGTCAGAGGTTTTCACCGTCATCACCGAA
CACAGTCTCCAAAAGTGGCAGTAGTGGCTT
3210
Leu Asn Glu Gly Asp Asp Gly Phe
Ter
rop
3220 3230 3240
ACGCGCGAGGCAGCTGCGGTAAAGCTCATC
TGCGCGCTCCGTCGACGCCATTTCGAGTAG
3240
Arg Ala Leu Cys Ser Arg Tyr Leu Glu Asp
rop
3250 3260 3270
AGCGTGGTCGTGAAGCGATTCACAGATGTC
TCGCACCAGCACTTCGCTAAGTGTCTACAG
3270
Ala His Asp His Leu Ser Glu Cys Ile Asp
rop
3280 3290 3300
TGCCTGTTCATCCGCGTCCAGCTCGTTGAG
ACGGACAAGTAGGCGCAGGTCGAGCAACTC
3300
Ala Gln Glu Asp Ala Asp Leu Glu Asn Leu
rop
3310 3320 3330
TTTCTCCAGAAGCGTTAATGTCTGGCTTCT
AAAGAGGTCTTCGCAATTACAGACCGAAGA
3330
Lys Glu Leu Leu Thr Leu Thr Gln Ser Arg
rop
3340 3350 3360
GATAAAGCGGGCCATGTTAAGGGCGGTTTT
CTATTTCGCCCGGTACAATTCCCGCCAAAA
3360
Ile Phe Arg Ala Met Asn Leu Ala Thr Lys
rop
3370 3380 3390
TTCCTGTTTGGTCACTGATGCCTCCGTGTA
AAGGACAAACCAGTGACTACGGAGGCACAT
3390
Glu Gln Lys Thr Val
rop
3400 3410 3420
AGGGGGATTTCTGTTCATGGGGGTAATGAT
TCCCCCTAAAGACAAGTACCCCCATTACTA
3420
3430 3440 3450
ACCGATGAAACGAGAGAGGATGCTCACGAT
TGGCTACTTTGCTCTCTCCTACGAGTGCTA
3450
3460 3470 3480
ACGGGTTACTGATGATGAACATGCCCGGTT
TGCCCAATGACTACTACTTGTACGGGCCAA
3480
3490 3500 3510
ACTGGAACGTTGTGAGGGTAAACAACTGGC
TGACCTTGCAACACTCCCATTTGTTGACCG
3510
3520 3530 3540
GGTATGGATGCGGCGGGACCAGAGAAAAAT
CCATACCTACGCCGCCCTGGTCTCTTTTTA
3540
3550 3560 3570
CACTCAGGGTCAATGCCAGCGCTTCGTTAA
GTGAGTCCCAGTTACGGTCGCGAAGCAATT
3570
3580 3590 3600
TACAGATGTAGGTGTTCCACAGGGTAGCCA
ATGTCTACATCCACAAGGTGTCCCATCGGT
3600
3610 3620 3630
GCAGCATCCTGCGATGCAGATCCGGAACAT
CGTCGTAGGACGCTACGTCTAGGCCTTGTA
3630
3640 3650 3660
AATGGTGCAGGGCGCTGACTTCCGCGTTTC
TTACCACGTCCCGCGACTGAAGGCGCAAAG
3660
3670 3680 3690
CAGACTTTACGAAACACGGAAACCAAAGAC
GTCTGAAATGCTTTGTGCCTTTGGTTTCTG
3690
3700 3710 3720
CATTCATGTTGTTGCTCAGGTCGCAGACGT
GTAAGTACAACAACGAGTCCAGCGTCTGCA
3720
3730 3740 3750
TTTGCAGCAGCAGTCGCTTCACGTTCGCTC
AAACGTCGTCGTCAGCGAAGTGCAAGCGAG
3750
3760 3770 3780
GCGTATCGGTGATTCATTCTGCTAACCAGT
CGCATAGCCACTAAGTAAGACGATTGGTCA
3780
3790 3800 3810
AAGGCAACCCCGCCAGCCTAGCCGGGTCCT
TTCCGTTGGGGCGGTCGGATCGGCCCAGGA
3810
3820 3830 3840
CAACGACAGGAGCACGATCATGCGCACCCG
GTTGCTGTCCTCGTGCTAGTACGCGTGGGC
3840
3850 3860 3870
TGGGGCCGCCATGCCGGCGATAATGGCCTG
ACCCCGGCGGTACGGCCGCTATTACCGGAC
3870
3880 3890 3900
CTTCTCGCCGAAACGTTTGGTGGCGGGACC
GAAGAGCGGCTTTGCAAACCACCGCCCTGG
3900
3910 3920 3930
AGTGACGAAGGCTTGAGCGAGGGCGTGCAA
TCACTGCTTCCGAACTCGCTCCCGCACGTT
3930
3940 3950 3960
GATTCCGAATACCGCAAGCGACAGGCCGAT
CTAAGGCTTATGGCGTTCGCTGTCCGGCTA
3960
3970 3980 3990
CATCGTCGCGCTCCAGCGAAAGCGGTCCTC
GTAGCAGCGCGAGGTCGCTTTCGCCAGGAG
3990
4000 4010 4020
GCCGAAAATGACCCAGAGCGCTGCCGGCAC
CGGCTTTTACTGGGTCTCGCGACGGCCGTG
4020
4030 4040 4050
CTGTCCTACGAGTTGCATGATAAAAAAGAC
GACAGGATGCTCAACGTACTATTTTTTCTG
4050
4060 4070 4080
AGTCATAAGTGCGGCGACGATAGTCATGCC
TCAGTATTCACGCCGCTGCTATCAGTACGG
4080
4090 4100 4110
CCGCGCCCACCGGAAGGAGCTGACTGGGTT
GGCGCGGGTGGCCTTCCTCGACTGACCCAA
4110
4120 4130 4140
GAAGGCTCTCAAGGGCATCGGTCGAGATCC
CTTCCGAGAGTTCCCGTAGCCAGCTCTAGG
4140
4150 4160 4170
CGGTGCCTAATGAGTGAGCTAACTTACATT
GCCACGGATTACTCACTCGATTGAATGTAA
4170
4180 4190 4200
AATTGCGTTGCGCTCACTGCCCGCTTTCCA
TTAACGCAACGCGAGTGACGGGCGAAAGGT
4200
Gln Gly Ser Glu Leu
Ter
lacI
4210 4220 4230
GTCGGGAAACCTGTCGTGCCAGCTGCATTA
CAGCCCTTTGGACAGCACGGTCGACGTAAT
4230
Arg Ser Val Gln Arg Ala Leu Gln Met Leu
lacI
4240 4250 4260
ATGAATCGGCCAACGCGCGGGGAGAGGCGG
TACTTAGCCGGTTGCGCGCCCCTCTCCGCC
4260
Ser Asp Ala Leu Ala Arg Pro Ser Ala Thr
lacI
4270 4280 4290
TTTGCGTATTGGGCGCCAGGGTGGTTTTTC
AAACGCATAACCCGCGGTCCCACCAAAAAG
4290
Gln Thr Asn Pro Ala Leu Thr Thr Lys Arg
lacI
4300 4310 4320
TTTTCACCAGTGAAACGGGCAACAGCTGAT
AAAAGTGGTCACTTTGCCCGTTGTCGACTA
4320
Lys Val Leu Ser Val Pro Leu Leu Gln Asn
lacI
4330 4340 4350
TGCCCTTCACCGCCTGGCCCTGAGAGAGTT
ACGGGAAGTGGCGGACCGGGACTCTCTCAA
4350
Gly Lys Val Ala Gln Gly Gln Ser Leu Gln
lacI
4360 4370 4380
GCAGCAAGCGGTCCACGCTGGTTTGCCCCA
CGTCGTTCGCCAGGTGCGACCAAACGGGGT
4380
Leu Leu Arg Asp Val Ser Thr Gln Gly Leu
lacI
4390 4400 4410
GCAGGCGAAAATCCTGTTTGATGGTGGTTA
CGTCCGCTTTTAGGACAAACTACCACCAAT
4410
Leu Arg Phe Asp Gln Lys Ile Thr Thr Leu
lacI
4420 4430 4440
ACGGCGGGATATAACATGAGCTATCTTCGG
TGCCGCCCTATATTGTACTCGATAGAAGCC
4440
Pro Pro Ile Tyr Cys Ser Ser Asp Glu Thr
lacI
4450 4460 4470
TATCGTCGTATCCCACTACCGAGATGTCCG
ATAGCAGCATAGGGTGATGGCTCTACAGGC
4470
Asp Asp Tyr Gly Val Val Ser Ile Asp Ala
lacI
4480 4490 4500
CACCAACGCGCAGCCCGGACTCGGTAATGG
GTGGTTGCGCGTCGGGCCTGAGCCATTACC
4500
Gly Val Arg Leu Gly Ser Glu Thr Ile Ala
lacI
4510 4520 4530
CGCGCATTGCGCCCAGCGCCATCTGATCGT
GCGCGTAACGCGGGTCGCGGTAGACTAGCA
4530
Arg Met Ala Gly Leu Ala Met Gln Asp Asn
lacI
4540 4550 4560
TGGCAACCAGCATCGCAGTGGGAACGATGC
ACCGTTGGTCGTAGCGTCACCCTTGCTACG
4560
Ala Val Leu Met Ala Thr Pro Val Ile Gly
lacI
4570 4580 4590
CCTCATTCAGCATTTGCATGGTTTGTTGAA
GGAGTAAGTCGTAAACGTACCAAACAACTT
4590
Glu Asn Leu Met Gln Met Thr Gln Gln Phe
lacI
4600 4610 4620
AACCGGACATGGCACTCCAGTCGCCTTCCC
TTGGCCTGTACCGTGAGGTCAGCGGAAGGG
4620
Gly Ser Met Ala Ser Trp Asp Gly Glu Arg
lacI
4630 4640 4650
GTTCCGCTATCGGCTGAATTTGATTGCGAG
CAAGGCGATAGCCGACTTAAACTAACGCTC
4650
Glu Ala Ile Pro Gln Ile Gln Asn Arg Thr
lacI
4660 4670 4680
TGAGATATTTATGCCAGCCAGCCAGACGCA
ACTCTATAAATACGGTCGGTCGGTCTGCGT
4680
Leu Tyr Lys His Trp Gly Ala Leu Arg Leu
lacI
4690 4700 4710
GACGCGCCGAGACAGAACTTAATGGGCCCG
CTGCGCGGCTCTGTCTTGAATTACCCGGGC
4710
Arg Ala Ser Val Ser Ser Leu Pro Gly Ala
lacI
4720 4730 4740
CTAACAGCGCGATTTGCTGGTGACCCAATG
GATTGTCGCGCTAAACGACCACTGGGTTAC
4740
Leu Leu Ala Ile Gln Gln His Gly Leu Ala
lacI
4750 4760 4770
CGACCAGATGCTCCACGCCCAGTCGCGTAC
GCTGGTCTACGAGGTGCGGGTCAGCGCATG
4770
Val Leu His Glu Val Gly Leu Arg Thr Gly
lacI
4780 4790 4800
CATCTTCATGGGAGAAAATAATACTGTTGA
GTAGAAGTACCCTCTTTTATTATGACAACT
4800
Asp Glu His Ser Phe Ile Ile Ser Asn Ile
lacI
4810 4820 4830
TGGGTGTCTGGTCAGAGACATCAAGAAATA
ACCCACAGACCAGTCTCTGTAGTTCTTTAT
4830
Pro Thr Gln Asp Ser Val Asp Leu Phe Leu
lacI
4840 4850 4860
ACGCCGGAACATTAGTGCAGGCAGCTTCCA
TGCGGCCTTGTAATCACGTCCGTCGAAGGT
4860
Ala Pro Val Asn Thr Cys Ala Ala Glu Val
lacI
4870 4880 4890
CAGCAATGGCATCCTGGTCATCCAGCGGAT
GTCGTTACCGTAGGACCAGTAGGTCGCCTA
4890
Ala Ile Ala Asp Gln Asp Asp Leu Pro Tyr
lacI
4900 4910 4920
AGTTAATGATCAGCCCACTGACGCGTTGCG
TCAATTACTAGTCGGGTGACTGCGCAACGC
4920
Asn Ile Ile Leu Gly Ser Val Arg Gln Ala
lacI
4930 4940 4950
CGAGAAGATTGTGCACCGCCGCTTTACAGG
GCTCTTCTAACACGTGGCGGCGAAATGTCC
4950
Leu Leu Asn His Val Ala Ala Lys Cys Ala
lacI
4960 4970 4980
CTTCGACGCCGCTTCGTTCTACCATCGACA
GAAGCTGCGGCGAAGCAAGATGGTAGCTGT
4980
Glu Val Gly Ser Arg Glu Val Met Ser Val
lacI
4990 5000 5010
CCACCACGCTGGCACCCAGTTGATCGGCGC
GGTGGTGCGACCGTGGGTCAACTAGCCGCG
5010
Val Val Ser Ala Gly Leu Gln Asp Ala Arg
lacI
5020 5030 5040
GAGATTTAATCGCCGCGACAATTTGCGACG
CTCTAAATTAGCGGCGCTGTTAAACGCTGC
5040
Ser Lys Ile Ala Ala Val Ile Gln Ser Pro
lacI
5050 5060 5070
GCGCGTGCAGGGCCAGACTGGAGGTGGCAA
CGCGCACGTCCCGGTCTGACCTCCACCGTT
5070
Ala His Leu Ala Leu Ser Ser Thr Ala Val
lacI
5080 5090 5100
CGCCAATCAGCAACGACTGTTTGCCCGCCA
GCGGTTAGTCGTTGCTGACAAACGGGCGGT
5100
Gly Ile Leu Leu Ser Gln Lys Gly Ala Leu
lacI
5110 5120 5130
GTTGTTGTGCCACGCGGTTGGGAATGTAAT
CAACAACACGGTGCGCCAACCCTTACATTA
5130
Gln Gln Ala Val Arg Asn Pro Ile Tyr Asn
lacI
5140 5150 5160
TCAGCTCCGCCATCGCCGCTTCCACTTTTT
AGTCGAGGCGGTAGCGGCGAAGGTGAAAAA
5160
Leu Glu Ala Met Ala Ala Glu Val Lys Glu
lacI
5170 5180 5190
CCCGCGTTTTCGCAGAAACGTGGCTGGCCT
GGGCGCAAAAGCGTCTTTGCACCGACCGGA
5190
Arg Thr Lys Ala Ser Val His Ser Ala Gln
lacI
5200 5210 5220
GGTTCACCACGCGGGAAACGGTCTGATAAG
CCAAGTGGTGCGCCCTTTGCCAGACTATTC
5220
Asn Val Val Arg Ser Val Thr Gln Tyr Ser
lacI
5230 5240 5250
AGACACCGGCATACTCTGCGACATCGTATA
TCTGTGGCCGTATGAGACGCTGTAGCATAT
5250
Val Gly Ala Tyr Glu Ala Val Asp Tyr Leu
lacI
5260 5270 5280
ACGTTACTGGTTTCACATTCACCACCCTGA
TGCAATGACCAAAGTGTAAGTGGTGGGACT
5280
Thr Val Pro Lys Val
lacI
lacI promoter
5290 5300 5310
ATTGACTCTCTTCCGGGCGCTATCATGCCA
TAACTGAGAGAAGGCCCGCGATAGTACGGT
5310
lacI promoter
5320 5330 5340
TACCGCGAAAGGTTTTGCGCCATTCGATGG
ATGGCGCTTTCCAAAACGCGGTAAGCTACC
5340
lacI promoter
5350 5360 5370
TGTCCGGGATCTCGACGCTCTCCCTTATGC
ACAGGCCCTAGAGCTGCGAGAGGGAATACG
5370
lacI promoter
5380 5390
GACTCCTGCATTAGGAAGCAGCC
CTGAGGACGTAATCCTTCGTCGG
5393